In Vitro Seeds Germination and Seedling Growth of Bambara Groundnut (Vigna subterranea (L.) Verdc. (Fabaceae))

Abstract

Bambara groundnut (Vigna subterranea (L.) Verdc.) is an indigenous grain legume. It occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa. Development of an efficient in vitro regeneration system, a prerequisite for genetic transformation application, requires the establishment of optimal conditions for seeds germination and plantlets development. Three types of seeds were inoculated on different basal media devoid of growth regulators. Various strengths of the medium of choice and the type and concentration of carbon source were also investigated. Responses to germination varied with the type of seed. Embryonic axis (EA) followed by seeds without coat (SWtC) germinated rapidly and expressed a high rate of germination. The growth performances of plantlets varied with the basal medium composition and the seeds type. The optimal growth performances of plants were displayed on half strength MS basal medium with SWtC and EA as source of seeds. Addition of 3% sucrose in the culture medium was more suitable for a maximum growth of plantlets derived from EA.

Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000)

“…Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea... As well as, it delayed the germination of all genotypes which have the ability to germination in this concentration compared with control 0%. It was noted there was a difference between genotypes in water imbibition rate because of osmotic stress caused on concentrations PEG, as Structural changes during imbibition typical found in genotypes were observed, this is consistent with studies (EL SHIMI et al, 1980;Koné et al, 2015;Saio et al, 1973;Swanson et al, 1985) which indicated reveals differences between legumes in water imbibition rate and both structural and textural changes occurring in legumes during imbibition to water.…”

Evaluation of the diversity in qualitative traits of Bambara groundnut germplasm (Vigna subterranea (L.) Verdc.) of Cte dIvoire

“…Bambara groundnut (Vigna subterranea) is an African crop widely grown by subsistence farmers. It is an underutilised food legume (Azam-Ali et al, 2001) that occupies a prominent place in the strategies to ensure food security in sub-Saharan Africa (Koné et al, 2015). Its edible seeds are an important source of calories, vitamins *Corresponding author.…”

In vitro germination and growth protocols of the ornamental Lophophora williamsii (Lem.) Coult. as a tool for protecting endangered wild populations

“…Therefore, similarly to germination efficiency, it looks like that full-strength MS may have a negative interaction with plantlets at the earliest stages of development, while it has no effect or even positive effect on later stages. Many studies have reported that 1/2×MS, or even 1/4×MS, provides a better effect not only in germination but also in subsequent early development than full MS (Gland-Zwerger, 1995;Xu et al, 2007;Manzur et al, 2013;Koné et al, 2015). This is probably due to a deleterious effect of some of the salts present in the MS formulation (Monnier, 1995).…”