In vitro Selection for Fusarium Wilt Resistance in Gladiolus

Abstract

Cormels pieces of four Fusarium susceptible Gladiolus cultivars (Friendship, Peter Pears, Victor Borge and Novalux) formed friable calli when cultured in vitro on Murashige and Skoog basal medium containing various concentrations of auxin and cytokinin. The friable calli established cell suspensions. Plantlet regeneration was obtained from the control callus, control cell suspension derived callus and in vitro selected Fusarium oxysporum Schlecht. resistant cell-lines of Friendship. The in vitro cormlets showed 85-95% germination after breaking dormancy of 8 weeks at 4 degrees C. Cell suspensions of all four Gladiolus cultivars were found to be highly sensitive to fusaric acid. Gradual increase in fusaric acid concentrations to the cell-suspension cultures decreased cell growth considerably. One albino plant was found from the second generation of the in vitro selected cell line of Friendship. The albino plant was found to be highly susceptible to F. oxysporum. The cormlets of all in vitro selected cell lines of Friendship were inoculated with a conidial suspension of the F. oxysporum before planting and were also sprayed with the same spore suspension for further characterization when the height of plants was about 6 cm. The four selected cell lines showed the same response whether or not they were inoculated with conidia of the F. oxysporum. Plantlets of all of the selected cell lines exhibited significant growth as compared with the control after application of conidia of the F. oxysporum.

Micropropagation of iridaceae—a review

“…Virus-eradication has been successfully reported (Simonsen and Hildebrandt 1971;Logan and Zettler 1985;Mangal et al 2002), and plantlets resistant to fusaric acid (produced by Fusarium oxysorum) were regenerated from suspension cultures by in vitro selection methods (Remotti et al 1997). Similar experiments using four different cultivars ('Friendship', Novalux', 'Peter Pears', and 'Victor Borge') yielded plantlets that displayed improved growth when inoculated with conidia or spores of F. oxysporum compared to unselected control cell lines (Nasir and Riazuddin 2008).…”

De novo Sequencing of the Leaf Transcriptome Reveals Complex Light-Responsive Regulatory Networks in Camellia sinensis cv. Baijiguan

“…Previous studies have demonstrated that the albino or yellow phenotype can result from multiple factors, including Chl biosynthesis or degradation (Lin et al, 2014 ), tetrapyrrole synthesis for heme and phytochromobilin (Yaronskaya et al, 2003 ), carotenoid biosynthesis, or degradation (Qin et al, 2007 ; Wang et al, 2009 ), chloroplast development and biogenesis (Peng et al, 2012 ; Su et al, 2012 ), signal transduction during chloroplast development (Sheng et al, 2014 ), disease resistance (Nasir and Riazuddin, 2008 ), reduced light-harvesting Chl proteins (LHCPs) (Asakura et al, 2008 ), and photo-oxidative stress (Miura et al, 2010 ; Zhan et al, 2014 ). Digital gene expression (DGE) analysis of the tea cultivar Anji Baicha identified several genes involved in Chl biosynthesis or chloroplast development (Ma et al, 2012 ).…”

<i>In Vitro</i> Mutation Induction and Selection of Chrysanthemum (<i>Dendranthema Grandiflora</i> Tzelev) Lines with Improved Resistance to <i>Septoria Obesa</i> Syd

“…The present study revealed the production of chrysanthemu m plants with greater resistance to leaf spot disease than the lines produced by other methods [17,20,21]. The improvement in leaf spot disease resistance using this protocol over a period of four years was more than achieved earlier [16].…”