<i>In Vitro</i>
            Selective Combinatory Effect of Ciprofloxacin with Nitroxoline, Sanguinarine, and Zinc Pyrithione against Diarrhea-Causing and Gut Beneficial Bacteria

Osei-Owusu, Hayford; Kudera, Tomas; Strakova, Marie; Rondevaldova, Johana; Skřivanová, E.; Nový, Pavel; Kokoška, Ladislav

doi:10.1128/spectrum.01063-22

Cited by 5 publications

(12 citation statements)

References 61 publications

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“…Streptomycin exerts inhibitory effects on gram-negative bacteria through inhibiting the synthesis of bacterial protein. The secretion of other bacteriocins namely phenyllactic acid, Janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin showed antimicrobial activity against pathogenic Escherichia coli CCTCC25922, Salmonella CVCC1880, and Staphylococcus aureus [ 43 – 45 ].…”

Section: Discussionmentioning

confidence: 99%

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Yang,

Shang,

Zhang

et al. 2023

BMC Microbiol

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Background As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. Results The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. Conclusion The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius.

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Section: Discussionmentioning

confidence: 99%

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Yang,

Shang,

Zhang

et al. 2023

BMC Microbiol

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“…The findings showed a bacteriostatic effect against the mentioned microorganisms but did not prove a bactericidal effect. Interestingly, an antagonistic effect against bifidobacteria (B. adolescentis, B. animalis, and B. breve) was found, which suggests a beneficial effect on the intestines during treatment [291]. Sanguinarine exhibits activity against MRSA and E. coli microorganisms and has antiinflammatory, anti-cancer, and antimicrobial properties.…”

Section: Enterococcus Sppmentioning

confidence: 99%

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

Pacyga,

Topola

et al. 2024

IJMS

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The rising prevalence of drug-resistant bacteria underscores the need to search for innovative and nature-based solutions. One of the approaches may be the use of plants that constitute a rich source of miscellaneous compounds with a wide range of biological properties. This review explores the antimicrobial activity of seven bioactives and their possible molecular mechanisms of action. Special attention was focused on the antibacterial properties of berberine, catechin, chelerythrine, cinnamaldehyde, ellagic acid, proanthocyanidin, and sanguinarine against Staphylococcus aureus, Enterococcus spp., Klebsiella pneumoniae, Acinetobacter baumannii, Escherichia coli, Serratia marcescens and Pseudomonas aeruginosa. The growing interest in novel therapeutic strategies based on new plant-derived formulations was confirmed by the growing number of articles. Natural products are one of the most promising and intensively examined agents to combat the consequences of the overuse and misuse of classical antibiotics.

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“…Sanguinarine, a benzylisoquinoline alkaloid isolated from the plant Sanguinaria canadensis [ 28 ], is commonly used in mouthwash and toothpaste to treat and prevent gingivitis and related inflammatory conditions [ 29 , 30 ]. It has demonstrated a synergistic effect against diarrhoeic pathogens when combined with ciprofloxacin [ 31 ]. Similarly, nitroxoline is a drug used to treat infectious and inflammatory urogenital tract diseases [ 32 ] and has showed synergism when used in combination with ciprofloxacin against strains of diarrhoea-causing bacteria [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has demonstrated a synergistic effect against diarrhoeic pathogens when combined with ciprofloxacin [ 31 ]. Similarly, nitroxoline is a drug used to treat infectious and inflammatory urogenital tract diseases [ 32 ] and has showed synergism when used in combination with ciprofloxacin against strains of diarrhoea-causing bacteria [ 31 ]. This agent is a derivative of 8-hydroxyquinoline, which is a compound previously detected in the plant Centaurea diffusa [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of In Vitro Synergistic Effects of Tetracycline with Alkaloid-Related Compounds against Diarrhoeic Bacteria

Osei-Owusu,

Rondevaldova,

Houdkova

et al. 2024

IJMS

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Diarrhoea remains an important public health concern, particularly in developing countries, and has become difficult to treat because of antibacterial resistance. The development of synergistic antimicrobial agents appears to be a promising alternative treatment against diarrhoeic infections. In this study, the combined effect of tetracycline together with either nitroxoline, sanguinarine, or zinc pyrithione (representing various classes of plant-based compounds) was evaluated in vitro against selected diarrhoeic bacteria (Enterococcus faecalis, Escherichia coli, Listeria monocytogenes, Shigella flexneri, Vibrio parahaemolyticus, and Yersinia enterocolitica). The chequerboard method in 96-well microtiter plates was used to determine the sum of the fractional inhibitory concentration indices (FICIs). Three independent experiments were performed per combination, each in triplicate. It was observed that the combination of tetracycline with either nitroxoline, sanguinarine, or zinc pyrithione produced synergistic effects against most of the pathogenic bacteria tested, with FICI values ranging from 0.086 to 0.5. Tetracycline–nitroxoline combinations produced the greatest synergistic action against S. flexneri at a FICI value of 0.086. The combinations of the agents tested in this study can thus be used for the development of new anti-diarrhoeic medications. However, studies focusing on their in vivo anti-diarrhoeic activity and safety are required before any consideration for utilization in human medicine.

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In Vitro Selective Combinatory Effect of Ciprofloxacin with Nitroxoline, Sanguinarine, and Zinc Pyrithione against Diarrhea-Causing and Gut Beneficial Bacteria

Cited by 5 publications

References 61 publications

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius

Bioactive Compounds from Plant Origin as Natural Antimicrobial Agents for the Treatment of Wound Infections

Evaluation of In Vitro Synergistic Effects of Tetracycline with Alkaloid-Related Compounds against Diarrhoeic Bacteria

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