<i>In vitro</i> simulation of biocompatibility of Ti‐Al‐V

Bruneel, Noël; Helsen, Jozef

doi:10.1002/jbm.820220305

Cited by 59 publications

(17 citation statements)

References 19 publications

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“…To understand the role that citrate plays in the leaching of Ti from Ti-containing implants, 19,20 a model for the blood speciation (pH 7.4) of Ti(IV) citrate was proposed (Supplementary Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

Unusual Synergism of Transferrin and Citrate in the Regulation of Ti(IV) Speciation, Transport, and Toxicity

Tinoco¹,

Saxena²,

Sharma³

et al. 2016

J. Am. Chem. Soc.

119

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Human serum transferrin (sTf) is a protein that mediates the transport of iron from blood to cells. Assisted by the synergistic anion carbonate sTf transports Fe(III) by binding the metal ion in a closed conformation. Previous studies suggest sTf’s role as a potential transporter of other metals like titanium. Ti is a widely used metal in colorants, foods, and implants. A substantial amount of Ti is leached into blood from these implants. However, the fate of the leached Ti and its transport into the cells is not known. Understanding Ti interaction with sTf assumes a greater significance with our ever increasing exposure to Ti in the form of implants. Based on in vitro studies it was speculated that transferrin can bind Ti(IV) assisted by a synergistic anion. However, the role and identity of the synergistic anion(s) and the conformational state in which sTf binds Ti(IV) are not known. Here we have solved the first X-ray crystal structure of a Ti(IV)-bound sTf. We find that sTf binds Ti(IV) in an open conformation with both carbonate and citrate as synergistic anions at the metal binding sites, an unprecedented role for citrate. Studies with cell lines suggest that Ti(IV)-sTf is transported into cells and that sTf and citrate regulate the metal’s blood speciation and attenuate its cytotoxic property. Our results provide the first glimpse into the citrate-transferrin synergism in the regulation of Ti(IV) bioactivity and offers insight into the future design of Ti(IV)-based anticancer drugs.

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Section: Resultsmentioning

confidence: 99%

Unusual Synergism of Transferrin and Citrate in the Regulation of Ti(IV) Speciation, Transport, and Toxicity

Tinoco¹,

Saxena²,

Sharma³

et al. 2016

J. Am. Chem. Soc.

119

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“…The adsorbed biomolecules raise the open-circuit potential of titanium with the increase in their adsorption density 3,4) and sometimes accelerate the dissolution of titanium. 5,6) Also, the biomolecules provide complex with alloying elements, leading to the selective dissolution of certain alloying elements 7,8) and to the change in the surface oxide composition of the alloys.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, effect of a length of immersion time is helpful to evaluate the corrosion resistance of the alloys for their long-term use in the body because some induction period was observed for the dissolution of alloying elements. 7) For the practical application of amorphous alloy, the bulk size over mm-order of the alloy is necessary. Some bulk amorphous alloys with mm-order size is fabricated with a casting method, 9) however the composition is rather limited by the stability of super cooled liquid.…”

Section: Introductionmentioning

confidence: 99%

Polarization Behavior of Bulk Zr-Base Amorphous Alloy Immersed in Cell Culture Medium

et al. 2002

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Effects of amino acids and proteins on polarization behavior of Zr-base amorphous alloy were examined using Hanks' solution, minimum essential medium (MEM), and cell culture medium (MEM+FBS) consisting with MEM and fetal bovine serum (FBS), respectively. Amorphous powder consolidated Zr 55 Al 10 Ni 10 Cu 15 alloy was cathodically and anodically polarized at the beginning of (after 1.8 ks) immersion and after a long-term (605 ks) immersion in each solution. Parameters on the anodic polarization curve; open-circuit potential (E open ), polarization resistance (R p ), and pitting potential (E pit ), were obtained for the examination of corrosion resistance. In the presence of amino acids and proteins, R p and E pit increased, indicating that resistance to both general and pitting corrosion was raised with those biomolecules. Thus, the adsorbed biomolecules probably work as a diffusion barrier to molecules and ions such as dissolved oxygen and phosphate and chloride ions. During immersion, the surface oxide film grew, leading to the increase in E open , average E pit , and R p although the parameters showed wide variance of deviation probably caused by the defects in alloy structure. The lowest values of E pit after the long-term immersion were not lower than those at the beginning of immersion, indicating that the sensitivity of defects to chloride ion was not enhanced during immersion. However, the pitting corrosion resistance decreased during immersion because the potential difference between E open and E pit decreased.

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“…These results agree with those published by Alberts et al (1990) and Messer et al (2001). Furthermore, the Ti can be selectively dissolved by the formation of complexes with proteins (Bruneel and Helsen, 1988;Clark and Williams, 1982).…”

Section: Resultsmentioning

confidence: 99%

Cell adhesion on Ti surface with controlled roughness

et al. 2015

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ABSTRACT:In this report, the in situ interaction between Saos-2 osteoblast cells and a smooth Ti surface was examined over time. The adhesion kinetics and mechanisms of cellular proliferation were monitored by quartz crystal microbalance (QCM) and electrochemical impedance spectroscopy (EIS). The rate of Saos-2 attachment on Ti surfaces, obtained from the measurements performed with the QCM, is a first-order reaction, with k=2.10. The impedance measurements indicate that in the absence of cells, the Ti resistance diminishes over time (7 days), due to the presence of amino acids and proteins from the culture medium that have been a dsorbed, while in the presence of osteoblasts, this decrease is much greater because of the compounds generated by the cells that accelerate the dissolution of Ti. RESUMEN: Adhesión de osteoblastos sobre una superficie de Ti de rugosidad controlada.En este trabajo, se ha estudiado la interacción in situ entre células osteoblásticas Saos-2 y una superficie de Ti de rugosidad controlada a lo largo del tiempo. El estudio de la cinética y los mecanismos de proliferación celular de adhesión se ha realizado a través de la microbalanza de cristal de cuarzo (QCM) y espectroscopía de impedancia electroquímica (EIS). La velocidad de adhesión de los osteoblastos sobre la superficie de Ti obtenida a través de medidas con la QCM, sigue una reacción de primer orden, con k=2×10 −3 min −1. Los ensayos de impedancia indican que, en ausencia de las células, la resistencia del Ti disminuye con el tiempo (7 días), debido a la presencia de aminoáci-dos y proteínas del medio de cultivo que se han adsorbido, mientras que en presencia de células, esta disminución es mucho mayor debido a los productos metabólicos generados por las células que aceleran la disolución del Ti.

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In vitro simulation of biocompatibility of Ti‐Al‐V

Cited by 59 publications

References 19 publications

Unusual Synergism of Transferrin and Citrate in the Regulation of Ti(IV) Speciation, Transport, and Toxicity

Unusual Synergism of Transferrin and Citrate in the Regulation of Ti(IV) Speciation, Transport, and Toxicity

Polarization Behavior of Bulk Zr-Base Amorphous Alloy Immersed in Cell Culture Medium

Cell adhesion on Ti surface with controlled roughness

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