<i>In vitro</i>solid-state production of<i>Steinernema innovationi</i>with cost analysis

Ramakuwela, Tshimangadzo; Hatting, Justin; Laing, Mark; Hazır, Selçuk; Thiebaut, Nicolene

doi:10.1080/09583157.2016.1159284

Cited by 25 publications

(12 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fortunately, the continuous optimization of media composition, inoculation procedures, cell density (Hirao and Ehlers, 2009), environmental conditions within fermenters and the overall down streaming process, (Chavarría-Hernández et al, 2006) has significantly increased the quality of EPNs produced via liquid culturing methods. There has been scant information published concerning quality control of EPN-based products; ten articles in the last three decades (Ramakuwela et al, 2016). In February 1999, a quality assessment study of commercially produced EPN's was conducted to determine how much the inconsistencies varied between different "cottage" (home based) producers.…”

Section: Discussionmentioning

confidence: 99%

Biological Control Technology Utilizing Heterorhabditis bacteriophora and Steinernema carpocapsae

Downs

Upadhyay

Mandjiny

et al. 2019

Int. J. Phytopathol.

View full text Add to dashboard Cite

A B S T R A C TEntomopathogenic nematodes (in the genus Steinernema and Heterorhabditis) have been studied and successfully commercialized as biological control agents. These organisms are highly virulent and safe for the non-target environment, animals and humans. For at least 200 target species, the nematode-bacteria complex has the potential to become a mass-marketed agricultural biopesticide. However, before nematodes can be successfully integrated into the agricultural system as a regular-use, "go-to" biopesticide, it is necessary to develop economical manufacturing processes. There are several manufacturing platforms: in vitro solid fermentation; in vitro liquid fermentation; and in vivo production. This review presents an analysis of each approach and discusses the advantages and disadvantages relative to the cost of production, technical expertise required, and quality of the final product.

show abstract

Section: Discussionmentioning

confidence: 99%

Biological Control Technology Utilizing Heterorhabditis bacteriophora and Steinernema carpocapsae

Downs

Upadhyay

Mandjiny

et al. 2019

Int. J. Phytopathol.

View full text Add to dashboard Cite

show abstract

“…Other developmental stages of S. ceratophorum were observed when calculating IJ yield after harvest. The inconsistency of developmental stages can be improved by optimization of media composition and the proportion of different components (Ramakuwela et al, 2016;Seenivasan, 2017). Inoculum concentration, age, and type might also affect IJ yield and IJ consistency (Shapiro-Ilan et al, 2014).…”

Section: Journal Of Nematologymentioning

confidence: 99%

Virulence of Steinernema ceratophorum against different pest insects and their potential for in vivo and in vitro culture

Yan

Chen

et al. 2021

Journal of Nematology

View full text Add to dashboard Cite

Entomopathogenic nematodes (EPN) are biological control agents used to control different insect pests. Indigenous EPN resources are important for the successful control of endemic pests. A native EPN isolate collected in Yunnan, China that showed high virulence against Spodoptera litura was identified and studied for virulence and production potential. The EPN isolate was identified as Steinernema ceratophorum and showed high virulence against different larval stages of Spodoptera exigua, Ostrinia nubilalis, Helicoverpa armigera, and the 2nd instar larvae of Spodoptera frugiperda when applied at 50 IJ/larva under in vitro conditions. In vivo culture of the S. ceratophorum isolate showed that Galleria mellonella larvae were the most suitable host compared to Tenebrio molitor and S. litura. The highest yield in in vivo culture was 1.15 × 10 5 IJ/larva when cultured in G. mellonella with inoculum concentration of 50 IJ/larva at 25°C. For in vitro solid culture, the highest yield for S. ceratophorum was 2.09 × 10 6 IJ/g when cultured at optimal temperature of 25°C. Nematodes from the in vivo and in vitro culture, both retained their virulence against the G. mellonella larvae. The S. ceratophorum showed high potential to be mass produced, which will enable the commercial use of this EPN isolate. This is the first attempt to mass culture the S. ceratophorum species. The study explores the usage of S. ceratophorum and provides basis for the commercialization of the EPN species.

show abstract

“…However, new endemic species or strains, which may have positive genetic traits, are important for mass production yield, effectiveness, host range, resistance to stress factors and other factors. Thus, considerable research has attempted to optimize in vitro liquid culture production of local species or strains (Upadhyay, 2015;Ramakuwela et al, 2016;Dunn et al, 2020). High liquid culture yield, high effectiveness or longer shelf life lowers commercial product expenses indirectly and contributes EPNs to compete with chemicals (Grewal, 2000;Shapiro-Ilan et al, 2012;Devi & George, 2018).…”

Section: Body Length Measurementmentioning

confidence: 99%

Comparison of hermaphrodites of hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and its parents on reproduction capacity

Ulu¹,

Özbudak²,

Düzenli³

et al. 2021

Turkish Journal of Entomology

View full text Add to dashboard Cite

Entomopathogenic nematodes (EPNs) are microscopic roundworms used in biocontrol. EPNs are obligate insect parasites, they live in soil, and they are especially effective against soilborne insects. They are a good alternative to chemical pesticides thanks to their advantages, such as prolonged longevity, broad host range and mass production suitability. However, EPNs cannot compete with chemical pesticides due to high production costs and short shelf life. The aim of this study was to determine the reproduction capacity of the Turkish hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and then compare it with its parents to improve its liquid culture yield. In this way, it is aimed to reveal the effects behind the high reproduction potential of the hybrid HBH strain. All experiments were performed at Bursa Uludağ University, Faculty of Agriculture, Department of Plant Protection, in 2020. All cadavers were periodically dissected, hermaphrodites were counted and their body lengths were measured. Compared to its parents, the hybrid HBH strain had greater hermaphrodite counts, with mean 66 individuals within 12 days, and hermaphrodite body length, with mean 3.88 mm. The results obtained from this study should provide information for commercial EPN production development.

show abstract

In vitrosolid-state production ofSteinernema innovationiwith cost analysis

Cited by 25 publications

References 28 publications

Biological Control Technology Utilizing Heterorhabditis bacteriophora and Steinernema carpocapsae

Biological Control Technology Utilizing Heterorhabditis bacteriophora and Steinernema carpocapsae

Virulence of Steinernema ceratophorum against different pest insects and their potential for in vivo and in vitro culture

Comparison of hermaphrodites of hybrid Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH strain and its parents on reproduction capacity

Contact Info

Product

Resources

About