<i>In vitro</i> studies for antiparasitic activities of <scp><i>Punica granatum</i></scp> extract

Al‐Shaebi, Esam M.; Al‐Quraishy, Saleh; Maodaa, Saleh N.; Abdel‐Gaber, Rewaida

doi:10.1002/jemt.24401

Cited by 2 publications

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References 65 publications

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“…Massive colonization of coccidia causes significant epithelial damage ( 3 ). The host may consequently suffer from diarrhea, malabsorption, inadequate weight gain, and overall poor performance ( 4 ). Also, coccidiosis leads to immune dysfunction and increases susceptibility to secondary bacterial infections, as it disrupts the balance of intestinal microflora ( 5 ).…”

Section: Introductionmentioning

confidence: 99%

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Abdel-Gaber,

Alamari,

Dkhil

et al. 2024

Front. Immunol.

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IntroductionRecently, the use of botanicals as an alternative to coccidiostats has been an appealing approach for controlling coccidiosis. Therefore, this study was conducted to evaluate the potential role of aqueous methanolic extract (200 mg/kg) of Krameria lappacea (roots) (KLRE) against infection induced by Eimeria papillata.MethodsA total of 25 male C57BL/6 mice were divided into five groups (I, II, III, IV, and V). On 1st day of the experiment, all groups except groups I (control) and II (non-infected-treated group with KLRE), were inoculated orally with 103 sporulated E. papillata oocysts. On the day of infection, group IV was treated with KLRE. Group V served as an infected-treated group and was treated with amprolium (coccidiostat).ResultsTreatment with extract and coccidiostat was continued for five consecutive days. While not reaching the efficacy level of the reference drug (amprolium), KLRE exhibited notable anticoccidial activity as assessed by key criteria, including oocyst suppression rate, total parasitic stages, and maintenance of nutrient homeostasis. The presence of phenolic and flavonoid compounds in KLRE is thought to be responsible for its positive effects. The Eimeria infection increased the oxidative damage in the jejunum. KLRE treatment significantly increased the activity of catalase and superoxide dismutase. On the contrary, KLRE decreased the level of malondialdehyde and nitric oxide. Moreover, KLRE treatment decreased macrophage infiltration in the mice jejunal tissue, as well as the extent of CD4 T cells and NFkB. E. papillata caused a state of systemic inflammatory response as revealed by the upregulation of inducible nitric oxide synthase (iNOs)-mRNA. Upon treatment with KLRE, the activity of iNOs was reduced from 3.63 to 1.46 fold. Moreover, KLRE was able to downregulate the expression of pro-inflammatory cytokines interferon-γ, nuclear factor kappa B, and interleukin-10 -mRNA by 1.63, 1.64, and 1.38 fold, respectively. Moreover, KLRE showed a significant reduction in the expression of IL-10 protein level from 104.27 ± 8.41 pg/ml to 62.18 ± 3.63 pg/ml.ConclusionCollectively, K. lappacea is a promising herbal medicine that could ameliorate the oxidative stress and inflammation of jejunum, induced by E. papillata infection in mice.

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Section: Introductionmentioning

confidence: 99%

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Abdel-Gaber,

Alamari,

Dkhil

et al. 2024

Front. Immunol.

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Potential role of the methanolic extract for Olea europaea (stem and leaves) on sporulation of Eimeria papillata oocysts: In vitro study

Al‐Shaebi,

Al‐Quraishy,

Abdel‐Gaber

2024

Biomedical Chromatography

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Many plants are efficient anticoccidial agents owing to their content of active chemicals. Drug‐resistant Eimeria species have emerged as a result of excessive drug use. The current work aimed to investigate the oocysticidal activity (Eimeria papillata) of Olea europaea stem extract (OESE) and leaf extract (OELE) in vitro. The results of gas chromatography–mass spectrometry analysis for OELE and OESE showed the presence of 12 and 9 phytochemical compounds, respectively. Also, chemical examination revealed that the plant extracts are rich in phenols, flavonoids and tannins. Additionally, the best radical scavenging activity of OESE and OELE was at a concentration of 100 μg/ml, reaching 92.04 ± 0.02 and 92.4 ± 0.2%, respectively. The in vitro study revealed that concentrations of 200 mg/ml from OESE and OELE caused significant inhibition (100%) of process sporulation for E. papillata oocysts, in contrast to the other commercial products, which displayed varying degrees of suppression sporulation. Our findings showed that OESE and OELE have anticoccidial activity, which motivates further the conduction of in vivo studies in the search for a less expensive and more efficient cure.

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In vitro studies for antiparasitic activities of Punica granatum extract

Cited by 2 publications

References 65 publications

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Krameria lappacea root extract’s anticoccidial properties and coordinated control of CD4 T cells for IL-10 production and antioxidant monitoring

Potential role of the methanolic extract for Olea europaea (stem and leaves) on sporulation of Eimeria papillata oocysts: In vitro study

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