<i>In vitro</i>studies of antagonistic fungi against the root-knot nematode,<i>Meloidogyne incognita</i>

Singh, Sanjay; Mathur, Nita

doi:10.1080/09583150903484318

Cited by 43 publications

(21 citation statements)

References 14 publications

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“…The tested antagonistic fungus, T. harzianum, was isolated from the egg mass of M. incognita. The observation that fungi isolated directly from root-knot nematode egg masses, as opposed to isolation from soil, tended to show higher levels of antagonism indicates that fungal origin is likely to be an important criterion when obtaining biological control agents (Affokpon et al, 2010;Singh & Mathur, 2010b).…”

Section: Discussionmentioning

confidence: 91%

Integrated approach for the management of the root-knot nematode, Meloidogyne incognita, on eggplant under field conditions

Singh

2013

Nematol

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A 2-year field study was conducted to develop an eco-friendly field application method for controlling root-knot disease of eggplant {Solanum melongena). The test sites were heavily infested with the root-knot nematode, Meloidogyne incognita. The efficacy of neem cake (1.5 t ha~'), talc-based preparations of Pseudomonas fluorescens (10 kg ha~') and Trichoderma harzianum (10 kg ha~') as soil application and seed treatment (10 g (kg seed)"') were tested to develop an integrated nematode management module against M. incognita infecting eggplant. Neem cake, P. fluorescens and T. harzianum alone and in combinations significantly reduced the incidence of root-knot disease of eggplant. Fresh and dry weight of shoots were higher in the plant where neem cake, P. fluorescens and T. harzianum had been apphed, than in both M. incogn/to-infected plants and other treatments. The best protection of disease, in terms of reduction in number of galls (81%) and reproductive factor (Pf/Pi < 0.5) of the nematode, was achieved through this treatment. It also enhanced yield of eggplant by up to 70%. It is suggested that integrated approach using organic amendment with bio-control agents to manage root-knot disease of eggplant under natural infestation is not only environmentally friendly but also more beneficial to growers. This approach also has potential for overcoming some of the efficacy problems that occur with application of individual biological control agent.

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Section: Discussionmentioning

confidence: 91%

Integrated approach for the management of the root-knot nematode, Meloidogyne incognita, on eggplant under field conditions

Singh

2013

Nematol

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“…Singh and Mathur (2010) In this study, substances were released on agar not only in plates containing P. redivivus, but also in control plates, indicating physiological stress or natural defense (Girotto et al, 2008). In this last case, these substances probably have properties against others organisms too, increasing the potential of Hohenbuehelia species as biological control agents, since many species from Basidiomycota are expected to produce antibiotic molecules (Robins et al, 1945).…”

Section: Resultsmentioning

confidence: 99%

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Lubian¹,

Martinha²,

Portz³

et al. 2018

JAS

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Biological control is a method of controlling pests through the use of other living organisms. The purposes of this study were to test Hohenbuehelia species as biological control agents against Panagrellus redivivus in vitro, evaluating nematodes influence on mycelia growth; establishing daily indexes for predation and growth and setting predation percentage. Five species previously identified as 436-Hohenbuehelia mastrucata (Nematoctonus hamatus), 528-H. bullulifera (not described so far), 581-H. paraguayensis (N. sp.), 582-H. sp. (N. sp.) and 631-H. portegna (N. campylosporus) were submitted to anamorphic purification directly from basidioma. Afterwards, 100 nematodes were added to each pure colony for predation test. Evaluation started right after 24 hours of nematode-fungus interaction. Immobilized and/or penetrated nematodes were counted and mycelia growth was measured. Results were subjected to variance analyses. Hohenbuehelia mastrucata had the best performance in growth speed, followed by H. portegna and H. paraguayensis; Nematodes multiplyied much but none specie grew more as an influence of their movement under mycelium, however all species formed trap devices and some of them produced adhesive or repelent substances. Trap devices were formed in control plates also. The plates of H. paraguayensis without nematodes grew more than treatments. Cumulative predation of H. portegna was the highest at 24 (195.5%) and 48 hours (235%). At the last evaluation day, H. paraguayensis preyed the same amount (185.75%) than H. portegna, followed by H. mastrucata (109.51%). Resulst of predation daily indexes displayed chronological activity for each isolate, where H. portegna was very reactive at first 24 hours, H. mastrucata raised its predacious activity in 48 hours being constant from this time on and H. paraguayensis pointed out itself at 72 hours. Other species presented low predation and growth indexes throughout experiment.

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“…Egg masses of M. incognita were picked up from pure culture pots of infected roots and placed into the sterilized plastic plates with sterile water and kept on the laboratory benches at room temperature (20)(21)(22)(23) o C) and allowed to hatch for 3-6 days. Two weeks old transplanted seedlings of tomato cv.…”

Section: Multiplication Extraction and Counting Number Of Juvenilesmentioning

confidence: 99%

“…Bio-control appears to offer an environmentally safe and ecologically feasible option for plant protection with great potential for promoting sustainable agriculture. The bio control efficiency depends on the nematode species, plant host and their root exudates, and other crops in rotation [23].The beneficial effects of certain types of plants derived materials and microorganisms in soil have been attributed to a decrease in the population densities of plant-parasitic nematodes [24].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Some Botanicals and Trichoderma harzianum for the Management of Tomato Root-knot Nematode (Meloidogyne incognita (Kofoid and White) Chit Wood)

Feyisa¹,

Lencho²,

Selvaraj³

et al. 2015

Adv Crop Sci Tech

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Root-knot nematode disease caused by Meloidogyne incognita (Kofoid and White) Chit wood) is one of the major constraints for successful cultivation of tomato (Lycopersicon esculentum Mill.) in Ethiopia. Hence, the present study was conducted to evaluate the effect of leaf and seed extracts of four botanicals viz., Rape seed (Brassica napus L.), Lantana (Lantana camara L.), African marigold (Tagetes erecta L.) and Neem (Azadirachta indica L.) at 5% and 10% concentrations and T. harzianum at 5% plus control were tested on root-knot nematode under in vitro and also to evaluate their against root-knot nematode development and their role on plant growth parameters of tomato under in vivo condition. Plant extracts were more effective and significantly inhibited egg hatching and immobilizing the J 2 larval mortality of M. incognita than T. harzianum. Aqueous extract of all the tested plants inhibited egg hatching of nematode and resulted 84.67-100% mortality of the J 2 juveniles of M. incognita in vitro at the 10% concentration after 72 h of exposure time. There were no significant differences among the treatments of rape seed leaf (84.7%) at 10% concentration and Lantana camara (87%), African marigold (86.3%) and Neem leaf (85%) at 5% concentration after 72 h. Aqueous seed extracts of A. indica more significantly inhibited egg hatching and larva mortality of the J 2 of M. incognita in vitro at the 10% concentration and immobilized by 89, 93 and 100% after 24, 48 and 72 h of exposures, respectively, while at similar concentration of T. erecta, B. napus and L. camara leaf extracts exhibited 92, 89 and 93.2% inhibition of egg hatching and 75, 62.1 and 73% larval mortality, respectively. The effect of different botanicals and T. harzianum singly and in combination were studied for the management of tomato root-knot nematode under greenhouse condition. There was a significant difference in the reduction of root-knot nematode incidence, root-knot nematode population, nematode reproduction rate (NRR), number of galls and egg masses per plant were recorded. In pot culture condition, the application of leaf extract of individual plant in the presence of the nematode significantly enhanced the growth of tomato seedlings in comparison to the control. A significant increase in plant height, shoot weight and root weight of the seedlings were observed at the 10% concentration of leaf extracts in comparison to control. There was a significant difference in the reduction of root-knot nematode population, nematode reduction rate, number of galls and egg masses per plant of L. camara combined with T. harzianum. The mean fruit weight and total yield were observed highest in the combination treatment of L. camara combined with T. harzianum. This study results revealed that the test plants are readily available to farmers at no cost and able to reduce nematode population below economic threshold.

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In vitrostudies of antagonistic fungi against the root-knot nematode,Meloidogyne incognita

Cited by 43 publications

References 14 publications

Integrated approach for the management of the root-knot nematode, Meloidogyne incognita, on eggplant under field conditions

Integrated approach for the management of the root-knot nematode, Meloidogyne incognita, on eggplant under field conditions

Daily Indexes for Predation and Growth of Nematophagous Mushrooms Species of Hohenbuehelia (Pleurotaceae) on Panagrellus redividus

Evaluation of Some Botanicals and Trichoderma harzianum for the Management of Tomato Root-knot Nematode (Meloidogyne incognita (Kofoid and White) Chit Wood)

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