In VitroStudies of Bacterial Cellulose and Magnetic Nanoparticles Smart Nanocomposites for Efficient Chronic Wounds Healing

Abstract

The quality of life of patients with chronic wounds can be extremely poor and, therefore, over the past decades, great efforts have been made to develop efficient strategies to improve the healing process and the social impact associated with these conditions. Cell based therapy, as a modern tissue engineering strategy, involves the design of 3D cell-scaffold bioconstructs obtained by preseeding drug loaded scaffolds with undifferentiated cells in order to achieve in situ functional de novo tissue. This paper reports on the development of bionanocomposites based on bacterial cellulose and magnetic nanoparticles (magnetite) for efficient chronic wounds healing. Composites were obtained directly in the cellulose bacterial culture medium by dispersing various amounts of magnetite nanoparticles during the biosynthesis process. After purification and drying, the membranes were characterized by Raman spectroscopy and X-ray diffraction to reveal the presence of magnetite within the bacterial cellulose matrix. Morphological investigation was employed through SEM and TEM analyses on bionanocomposites. The biocompatibility of these innovative materials was studied in relation to human adipose derived stem cells in terms of cellular morphology, viability, and proliferation as well as scaffolds cytotoxic potential.

A Novel “Microbial Bait” Technique for Capturing Fe(III)-Reducing Bacteria

“…Transmission Electron Microscope analysis of the deployed Fe(III)-coated pumice slurries amended with electron donors indicated the presence of spherical nanoparticles with a diameter of 6 nm consistent with the morphological features of magnetite (Galateanu et al, 2015; Figure 6A). These morphological features are similar to the TEM images of biogenic magnetite crystals observed by Cutting et al (2009), where akaganeite was reduced to magnetite by Geobacter sulfurreducens in cultures amended with 10 µm AQDS and 20 mM sodium acetate, and incubated for 6 days.…”

Metallic Nanoparticles, Toxicity Issues and Applications in Medicine

“…In an independent study, the bacterial cellulose incorporated with Fe 3 O 4 MNPs has been used for treatment of chronic wounds. The nanocomposites have been found to be biocompatible to human adipose stem cells in terms of viability, cytotoxicity, proliferation and cellular morphology (Galateanu et al 2015).…”

Effect of carbon sources on physicochemical properties of bacterial cellulose produced from Gluconacetobacter xylinus MTCC 7795

“…Owing to its unique properties, BC has found application in a number of fields such as the paper and textile industry (7), diaphragms for electro-acoustic transducers, the food industry (9, 10), film coatings as drug delivery systems (11), pharmaceuticals and cosmetics, optically transparent composites (12,13), substrates for OLEDs (13), biomaterials in cosmetics and medicine (11,(14)(15)(16). In recent years, biomedical applications of BC have received considerable attention in the literature, particulary in wound dressing, blood vessels, vascular grafts and delivery systems of drugs and proteins (17).…”

“…In recent years, biomedical applications of BC have received considerable attention in the literature, particulary in wound dressing, blood vessels, vascular grafts and delivery systems of drugs and proteins (17). Various studies have proven the application of BC scaffolds in the field of tissue engineering and wound healing (15). Saska et al (2011) reported that BC composites containing hydroxyapatite showed bone regeneration properties and could ideally be used as bone grafts (16).…”