<i>In vitro</i> transfer of keratinocytes: Comparison of transfer from fibrin membrane and delivery by aerosol spray

Duncan, C. O.; Shelton, Richard M.; Navsaria, Harshad; Balderson, D.; Papini, Remo; Barralet, Jake E.

doi:10.1002/jbm.b.30198

Cited by 35 publications

(32 citation statements)

References 28 publications

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“…adhere, but the proliferation capacity seemed poor and the cultures never recovered to reach the cell yield of the other methods even after 14 days of culture. This could be the result of different stresses, presented by Duncan et al [12], as it is likely that the cells sustained at least elongation stress by being smeared across the surface and that this in turn would lead to reduced cellular metabolism and proliferative capacity, which was also described by Harkin et al [11]. The Harvest ® technique gave a cell survival of about 70%…”

Section: Discussionmentioning

confidence: 87%

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Fredriksson

Kratz

Huss

2008

Burns

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Transplantation of autologous cultured keratinocytes in single cell suspension is useful in the treatment of burns. The reduced time needed for culture, and the fact that keratinocytes in suspension can be transported from the laboratory to the patient in small vials, thus reducing the costs involved and be stored (frozen) in the clinic for transplantation when the wound surfaces are ready, makes it appealing. We found few published data in the literature about actual cell survival after transplantation of keratinocytes in single cell suspension and so did a comparative in vitro study, considering commonly used application techniques. Human primary keratinocytes were transplanted in vitro in a standard manner using different techniques. Keratinocytes were counted before and after transplantation, were subsequently allowed to proliferate, and counted again on days 4, 8, and 14 by vital staining. Cell survival varied, ranging from 47% to >90%, depending on the technique. However, the proliferation assays showed that the differences in numbers diminished after 8 days of culture. Our findings indicate that a great number of cells die during transplantation but that this effect is diminished if cells are allowed to proliferate in an optimal milieu. A burned patient's wounds cannot be regarded as the optimal milieu, and using less harsh methods of transplantation may increase the take rate and wound closing properties of autologous keratinocytes transplanted in a single cell suspension.

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Section: Discussionmentioning

confidence: 87%

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Fredriksson

Kratz

Huss

2008

Burns

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“…Since the early 1980s, epidermal grafts have been created by the in vitro culture of keratinocyte cells isolated from small biopsies. 41,112 In general, there are four techniques: keratinocytes grown into integrated cell sheets, 53 made into suspensions or injections, 166 sprayed onto wound sites , 28,34 or delivered on various support carriers, scaffolds, or dressings. 162,164 Some of them are available commercially under different name brands.…”

Section: Cell Transplantation As Regenerative Therapy For the Skin-immentioning

confidence: 99%

“…110 Spraying the cells may minimize the culture time and would also be easy to implement if the areas around the implant have not formed an epidermal seal. Techniques to increase cell numbers or the use of autologous cells may also be used for cell spraying or injection 34,158 because there is evidence that extensive keratinocyte culture before implantation limits keratinocyte survival.…”

Section: Cell Transplantation As Regenerative Therapy For the Skin-immentioning

confidence: 99%

Bioengineering the Skin–Implant Interface: The Use of Regenerative Therapies in Implanted Devices

Peramo

Marcelo

2010

Ann Biomed Eng

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This discussion and review article focuses on the possible use of regenerative techniques applied to the interfaces between skin and medical implants. As is widely known, the area of contact between an implant and the skin--the skin-implant interface--is prone to recurrent and persistent problems originated from the lack of integration between the material of the implant and the skin. Producing a long-term successful biointerface between skin and the implanted device is still an unsolved problem. These complications have prevented the development of advanced prosthetics and the evolution of biointegrated devices with new technologies. While previous techniques addressing these issues have relied mostly on the coating of the implants or the modification of the topology of the devices, recent in vitro developed techniques have shown that is possible to introduce biocompatible and possibly regenerative materials at the skin-device interface. These techniques have also shown that the process of delivering the materials has biological effects on the skin surrounding the implant, thus converting bioinert into bioactive, dynamic interfaces. Given that the best clinical outcome is the long-term stabilization and integration of the soft tissue around the implant, this article presents the basis for the selection of regenerative materials and therapies for long-term use at the skin-device interface, with focus on the use of natural biopolymers and skin cell transplantation.

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“…Suitability of cell spraying was already shown for external/superficial applications e.g. treatment of burn injuries by [5], treatment of topographically challenging wounds by [6] and most recently in [7]. However, application of cell-sprays within the human body was not realized so far.…”

Section: Introductionmentioning

confidence: 99%

Viability of coaxial atomization for disintegration of cell solutions in cell spray applications

Bieber

Menzel

Thiebes

et al. 2017

Proceedings ILASS–Europe 2017. 28th Conference on Liquid Atomization and Spray Systems

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Treating Leukemia with intravenous stem cell transplantation represents a well-established therapy technique. For applications, that require high local cell concentrations, transplantation by conventional intravenous injection is less potent, due to cell distribution with blood circulation. Instead, spraying them directly onto the injured or diseased area shows promising results in various applications, e.g. superficial treatment of topographically challenging wounds, in situ seeding of cells on implants, deposition of cells in tubular organs for stem cell therapy. The present work aims for a basic knowledge about viability boundaries for coaxial cell-spray atomization and the reciprocal influence between cells in solution and primary breakup mechanics. A generic modular nozzle is developed, to ensures reproducible boundary conditions. Investigations are conducted regarding primary breakup and relations between resulting droplet size distribution and cell survival. Measurements are performed, utilizing microscopic high-speed visualization with suitable image post processing. Cell viability is analyzed using phase contrast microscopy prior and after atomization. A relation between Rayleigh-Taylor instability wavelength and droplet size distributions by means of Sauter mean diameter (SMD) and cell survival rate (CSR) is suggested. A power law is presented, exclusively dependent on dimensionless measures (λ ⊥ ∼ Re −1/2 We −1/3 ) which is found to be proportional to SMD and CSR. Keywords cell spray, coaxial atomization, cell survival IntroductionStem cell therapy is a research field in regenerative medicine with increasing importance due to the ability of cells to support healing of afflicted tissue. A well-established example represents the treatment of Leukemia by means of intravenous stem cell transplantation, where cells distribute by blood circulation within the organism after injection. To utilize the full potential of stem cells for applications that require high local cell concentration, alternative approaches for transplantation would be desirable, that enable local treatment rather than homogeneous distribution. An example for the requirement of high concentrations is the treatment of Acute Lung Injury (ALI) or Acute Respiratory Distress Syndrome (ARDS), both resulting from inhalation of biological toxins or toxic chemicals [1]. Despite extensive research, the mortality rate remains high (40%) for both diagnoses [2]. As stated in [3], the anti-inflammatory properties of intrapulmonary instilled MSCs decrease the severity of endotoxin-induced ALI and improves survival in animal studies, though the increased liquid feed may further damage the lung tissue. Minimization of liquid feed into the lung could be achieved by using droplets as a carrier medium. A well-established technique for drug delivery in the lung using droplets, is the nebulization of liquids into a fine mist, that is directly inhaled. However, nebulization of cell solutions leads to high cell mortality rates [4]. In addition, direct in...

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In vitro transfer of keratinocytes: Comparison of transfer from fibrin membrane and delivery by aerosol spray

Cited by 35 publications

References 28 publications

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Transplantation of cultured human keratinocytes in single cell suspension: A comparative in vitro study of different application techniques

Bioengineering the Skin–Implant Interface: The Use of Regenerative Therapies in Implanted Devices

Viability of coaxial atomization for disintegration of cell solutions in cell spray applications

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