In VitroVirucidal Effects ofAllium sativum(Garlic) Extract and Compounds

Abstract

Garlic (Allium sativum) has been shown to have antiviral activity, but the compounds responsible have not been identified. Using direct pre-infection incubation assays, we determined the in vitro virucidal effects of fresh garlic extract, its polar fraction, and the following garlic associated compounds: diallyl thiosulfinate (allicin), allyl methyl thiosulfinate, methyl allyl thiosulfinate, ajoene, alliin, deoxyalliin, diallyl disulfide, and diallyl trisulfide. Activity was determined against selected viruses including, herpes simplex virus type 1, herpes simplex virus type 2, parainfluenza virus type 3, vaccinia virus, vesicular stomatitis virus, and human rhinovirus type 2. The order for virucidal activity generally was: ajoene > allicin > allyl methyl thiosulfinate > methyl allyl thiosulfinate. Ajoene was found in oil-macerates of garlic but not in fresh garlic extracts. No activity was found for the garlic polar fraction, alliin, deoxyalliin, diallyl disulfide, or diallyl trisulfide. Fresh garlic extract, in which thiosulfinates appeared to be the active components, was virucidal to each virus tested. The predominant thiosulfinate in fresh garlic extract was allicin. Lack of reduction in yields of infectious virus indicated undetectable levels of intracellular antiviral activity for either allicin or fresh garlic extract. Furthermore, concentrations that were virucidal were also toxic to HeLa and Vero cells. Virucidal assay results were not influenced by cytotoxicity since the compounds were diluted below toxic levels prior to assaying for infectious virus. These results indicate that virucidal activity and cytotoxicity may have depended upon the viral envelope and cell membrane, respectively. However, activity against non-enveloped virus may have been due to inhibition of viral adsorption or penetration.(ABSTRACT TRUNCATED AT 250 WORDS)

Scavenging Activity of Different Garlic Extracts and Garlic Powder and their Antioxidant Effect on Heated Sunflower Oil

“…It has many medicinal effects such as lowering of blood cholesterol level (Yeh and Yeh, 1994), antiplatelet aggregation (Steiner et al, 1996), anti-inflammatory activity (Baek et al, 2001) and inhibition of cholesterol synthesis (Piscitelli et al, 2002). Garlic has been long known to have antibacterial (Rees et al, 1993;Sasaki et al, 1999;Shobana et al, 2009), antifungal (Pai and Platt, 1995), anticancer (Unnikrishnan and Kuttan, 1990;Kaschula et al, 2010), antioxidant (Galano and Francisco-Marquez, 2009) and antiviral (Weber et al, 1992).…”

Trials to improve the response of Orechromis niloticus to Aeromonas hydrophila vaccine using immunostimulants (garlic, Echinacea) and probiotics (Organic GreenTM and Vet-YeastTM)

“…It was previously stated that, immunostimulants improve the protection of fish against diseases by enhancing nonspecific and humoral defense mechanisms (Sakai, 1999). Garlic has been effect against many bacteria (Ress et al, 1993), fungi (Adetumbi et al, 1986) and viruses (Weber et al, 1992).…”

Effect of temperature on antibiotic properties of garlic (Allium sativum L.) and ginger (Zingiber officinale Rosc.)

“…A. sativum, the common garlic, has traditional dietary and medicinal applications as an antiinfective agent (Lawson, 1998;Reuter et al, 1996). In vitro evidence of the antimicrobial activity of fresh and freeze-dried garlic extracts against many bacteria (Cavallito and Bailey, 1944;Rees et al, 1993), fungi (Adetumbi et al, 1986), and viruses (Weber et al, 1992) lends support to these applications. On the other hand, Z. officinale Rosc.…”