In Vitro α‐Amylase and α‐Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves

Kifle, Zemene Demelash; Debeb, Simachew Gidey; Belayneh, Yaschilal Muche

doi:10.1155/2021/6652777

Cited by 21 publications

(13 citation statements)

References 47 publications

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“…Ethyl acetate is a semi-polar solvent that is often used as a solvent because it can attract many compounds, both polar and non-polar compounds. This is supported by the research of Kifle et al (2021) that the ethyl acetate solvent used to extract the α-amylase inhibitor had an inhibition percentage of 61.57% (IC50, 18.73 g/ml), greater than chloroform solvent with a percentage inhibition value of 56.87% (IC50, 21.57 g /ml).…”

Section: An α-Amylase Extract Ethyl Acetate Inhibitor Activity Testmentioning

confidence: 69%

Production of α-Amylase Inhibitors of Aspergillus RD2 from Dewandaru (Eugenia uniflora L.) as Diabetes Drug

Lutfiyah

2022

J Bio Bio Edu

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Diabetes mellitus is a metabolic disorder characterized by above normal blood glucose levels. A α-amylase inhibitors have inhibitory activity against α-amylase enzymes and cause decrease in glucose absorption. Dewandaru (E. uniflora L.) has the potential to produce compounds capable of controlling blood glucose levels. Aspergillus RD2 from twigs of the Dewandaru plant is expected to be able to produce antidiabetic compounds. This study aimed to determine the ability of the Aspergillus RD2 to produce α-amylase inhibitors by determining the production time, extracting α-amylase inhibitor compounds, testing the activity of the supernatant αα-amylase inhibitor and ethyl acetate extract with various concentrations of 2%, 4%, 6%, 8%, 10% and GC-MS analysis. The results showed the Aspergillus RD2 was able to produce αα-amylase inhibitors with an inhibitory activity of 59.71%. The incubation time of the Aspergillus RD2 in producing the highest α-amylase inhibitor was on the 7th day. The highest α-amylase inhibitor activity was at a concentration of 6% extract with an inhibition percentage of 82.79%. 9-Octadecenoic acid, 9-Octadecenal and n-Hexadecanoic acid were identified as having α-amylase inhibitor and antidiabetic activity. Aspergillus RD2 is able to produce α-amylase inhibitor compounds that have the potential to be used as antidiabetic drugs.

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Section: An α-Amylase Extract Ethyl Acetate Inhibitor Activity Testmentioning

confidence: 69%

Production of α-Amylase Inhibitors of Aspergillus RD2 from Dewandaru (Eugenia uniflora L.) as Diabetes Drug

Lutfiyah

2022

J Bio Bio Edu

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“…At concentration of 500 g/ml, the crude extract showed percentage inhibition of 74.52 %, with an IC 50 of 14.52 μg.mL -1 . The highest α-glucosidase inhibitory activity was shown by the crude extract and aqueous fraction (IC 50 : 15.89 μg.mL -1 and 11.67 μg.mL -1 , respectively) [43].…”

Section: Hagenia Abyssinicamentioning

confidence: 95%

A Review on the Antidiabetic Potential of Medicinal Plants

Bushnak¹,

Hajj²,

Jaber³

2021

SJMH

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Diabetes mellitus has long been seen as a substantial economic burden on patients, their families, and society. Impairment in blood sugar regulation has major health repercussions. Furthermore, untreated diabetes causes major chronic complications like blindness, renal failure, and heart failure, as well as an increase in associated mortality. New anti-diabetic medicines are being researched to help alleviate this issue. Conventional Anti-diabetic medications are beneficial, several synthetic drugs are available in the market to treat diabetes, but they are costly and come with inevitable adverse effects. Medicinal plants, on the other hand, may serve as an alternate source of anti-diabetic agents. According to the World Health Organization, 80 % of the population in underdeveloped nations still relies on traditional medicines or folk medicines, which are largely made from plants, for disease prevention or treatment. For instance, anti- proliferative, anti-viral, anti-inflammatory and anti-hyperglycemic effects. In order to find a natural anti-diabetic source that comes with less side effects, several studies have been conducted. The aim of this work is to review these studies and highlight the potential of plants when it comes to their anti-diabetic effect.

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“…Mendonca & E.P. Sousa crude extracts according to published procedures [19,20]. Briefly, 50 μL of crude root and bark extracts at varying concentrations (0-400 μg/mL) was mixed with 10 μL of α-glucosidase from Aspergillus, Niger (Sigma-Aldrich, South Africa) (1 U/mL) and 125 μL of 20 mM sodium phosphate buffer, pH 6.9.…”

Section: Methodsmentioning

confidence: 99%

Inhibitory andin silicomolecular docking ofXeroderris stuhlmannii(Taub.) Mendonca & E.P. Sousa phytochemical compounds on human α-glucosidases

Nyathi

Bvunzawabaya

Manzombe

et al. 2022

Preprint

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Millions of people in developing nations rely on herbal traditional medicine for the treatment of ailments such as diabetes mellitus, stomach disorders and respiratory diseases. Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa is a medicinal plant used traditionally in Zimbabwe to treat diabetes mellitus and its complications. However, there is no scientific evidence to support its role as an antidiabetic medicinal plant. Here we hypothesized that Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa contain bioactive phytochemicals that can scavenge free radicals, and inhibit digestive enzymes that contribute to type 2 diabetes mellitus (T2DM). To test this hypothesis, we examined the free radical scavenging potential of crude extracts using the diphenyl-2-picrylhydrazyl assay in vitro. Furthermore, we carried out in vitro antidiabetic activity of crude extracts using chromogenic 3,5-dinitrosalicylic acid and p-nitrophenyl-alpha-D-glucopyranoside substrates on alpha-amylase and alpha-glucosidase. In addition, we used molecular docking approaches to screen for bioactive phytochemical compounds targeting the digestive enzymes. Our results showed that phytochemicals in Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa extracts scavenged free radicals with IC50 values ranging from 0.011-0.013 micrograms/mL. Further, the crude extracts significantly inhibited alpha-amylase and alpha-glucosidase with IC50 values of 12.9-21.1 micrograms/mL and 8.8-16.0 micrograms/mL, respectively. In silico molecular docking findings and pharmacokinetic predictions showed that myricetin is a novel inhibitor of the digestive enzymes that contributes to high blood glucose. Collectively, our findings suggest pharmacological targeting of digestive enzymes by Xeroderris stuhlmannii (Taub). Mendonca & E.P. Sousa crude extracts could lesion type 2 mellitus complications in humans.

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In Vitro α‐Amylase and α‐Glucosidase Inhibitory and Antioxidant Activities of the Crude Extract and Solvent Fractions of Hagenia abyssinica Leaves

Cited by 21 publications

References 47 publications

Production of α-Amylase Inhibitors of Aspergillus RD2 from Dewandaru (Eugenia uniflora L.) as Diabetes Drug

Production of α-Amylase Inhibitors of Aspergillus RD2 from Dewandaru (Eugenia uniflora L.) as Diabetes Drug

A Review on the Antidiabetic Potential of Medicinal Plants

Inhibitory andin silicomolecular docking ofXeroderris stuhlmannii(Taub.) Mendonca & E.P. Sousa phytochemical compounds on human α-glucosidases

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