In vivo activation of plasma membrane H+‐ATPase hydrolytic activity by complex lipid‐bound unsaturated fatty acids in Ustilago maydis

Hernández, Agustín; Cooke, David T.; Clarkson, David T.

doi:10.1046/j.0014-2956.2001.02738.x

Cited by 13 publications

(8 citation statements)

References 42 publications

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“…Similarly Ros et al (1992) and Fodor et al (1995) have indicated that saturation of fatty acid in the membrane of plants growing with metals increased, whereas the level of sterols significantly decreased. Since lipid composition and membrane fluidity are considered as significant factors regulating the plasma membrane H + -ATPase ( Hernandez et al , 2002 ), an inactivation of the proton pump could be the result of the metal-induced changes in the plasma membrane.…”

Section: Discussionmentioning

confidence: 99%

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots

Janicka

Kabała

Burzyński

et al. 2008

Journal of Experimental Botany

179

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The effect of heavy metals on the modification of plasma membrane H+-ATPase (EC 3.6.3.14) activity in cucumber roots was studied. In plants stressed for 2 h with 10 μM or 100 μM Cd, Cu or Ni the hydrolytic as well as the transporting activity of H+-ATPase in the plasma membranes of root cells was decreased. Transcript levels of Cucumis sativus plasma membrane H+-ATPase in roots treated with 10 μM Cd, Cu, or Ni as well as with 100 μM Cu or Ni were similar to the control, indicating that the action of metals did not involve the gene expression level. Only in roots exposed to 100 μM Cd was the level of plasma membrane H+-ATPase mRNA markedly decreased. The inhibition of the plasma membrane proton pump caused by 100 μM Cd, Cu and Ni was partially diminished in the presence of cantharidin, a specific inhibitor of protein phosphatases. Western blot analysis with the antibody against phosphothreonine confirmed that decreased activity of plasma membrane H+-ATPase under heavy metals resulted from dephosphorylation of the enzyme protein. Taken together, these data strongly indicated that alteration of the enzyme under heavy metal stresses was mainly due to the post-translational modification of its proteins in short-term experiments.

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Section: Discussionmentioning

confidence: 99%

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots

Janicka

Kabała

Burzyński

et al. 2008

Journal of Experimental Botany

179

View full text Add to dashboard Cite

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“…A similar effect was observed in the case of Cu in tomato membrane lipids as a result of 7 days' stress (Ouariti et al, 1997). It is known that PM H + -ATPase is stimulated when the degree of unsaturation of fatty acids is increased (Hernandez et al, 2002). In soybean root, under aluminium stress, up-regulation of transcript, translation and threonine-oriented phosphorylation of plasma membrane H + -ATPase was observed (Shen et al, 2005).…”

Section: Heavy Metalsmentioning

confidence: 92%

“…The activation of H + -ATPase is dependent on the degree of saturation or unsaturation of the fatty acyl chain and its length. The activity decreased with an increase in the length of the fatty acyl chain and in the degree of unsaturation of fatty acid (Hernandez et al, 2002;Kasamo, 2003, Martz et al, 2006. Lysophosphatidylcholine (a natural detergent produced from phosphatidylcholine by phospholipase A 2 ) increased the plasma membrane H + -ATPase activity (Pedechenko et al, 1990, Regenberg et al, 1995.…”

Section: Regulation By Membrane Environmentmentioning

confidence: 99%

Plant Plasma Membrane H+-ATPase in Adaptation of Plants to Abiotic Stresses

Janicka¹

2011

Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives

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“…In potato leaves, the increase in PM H + -ATPase activity could be explained by a simultaneous increase in 18:2 FA in these tissues (Palta and Meade, 1989). A high correlation between PM H + -ATPase activity and 18:3 FA content, or the 18:3/18:2 ratio, was measured in PMs of rye roots (White et al 1990, Hernández et al 2002. In the fungus Ustilago maydis, the 18:2/18:1 ratio is highly correlated with the activation of PM H + -ATPase (Hernández et al 2002).…”

Section: Role Of Fas In the Regulation Of Pm H + -Atpase Activitymentioning

confidence: 99%

Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation

Martz¹,

Sutinen²,

Kiviniemi³

et al. 2006

Tree Physiology

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It has previously been suggested that plasma membrane ATPase (PM H+-ATPase, EC 3.6.1.3.) is a site of incipient freezing injury because activity increases following cold acclimation and there are published data indicating that activity of PM H+-ATPase is modulated by changes in lipids associated with the enzyme. To test and extend these findings in a tree species, we analyzed PM H+-ATPase activity and the fatty acid (FA) composition of glycerolipids in purified plasma membranes (PMs) prepared by the two-phase partition method from current-year needles of adult red pine (Pinus resinosa Ait.) trees. Freezing tolerance of the needles decreased from -56 degrees C in March to -9 degrees C in May, and increased from -15 degrees C in September to -148 degrees C in January. Specific activity of vanadate-sensitive PM H+-ATPase increased more than two-fold following cold acclimation, despite a concurrent increase in protein concentration. During de-acclimation, decreases in PM H+-ATPase activity and freezing tolerance were accompanied by decreases in the proportions of oleic (18:1) and linoleic (18:2) acids and increases in the proportions of palmitic (16:0) and linolenic (18:3) acids in total glycerolipids extracted from the plasma membrane fraction. This pattern of changes in PM H+-ATPase activity and the 18:1, 18:2 and 18:3 fatty acids was reversed during cold acclimation. In the PM fractions, changes in FA unsaturation, expressed as the double bond index (1 x 18:1 + 2 x 18:2 + 3 x 18:3), were closely correlated with changes in H+-ATPase specific activity (r2 = 0.995). Changes in freezing tolerance were well correlated with DBI (r2 = 0.877) and ATPase specific activity (r2 = 0.833) in the PM fraction. Total ATPase activity in microsomal fractions also closely followed changes in freezing tolerance (r2 = 0.969). We conclude that, as in herbaceous plants, simultaneous seasonal changes in PM H+-ATPase activity and fatty acid composition occur during cold acclimation and de-acclimation in an extremely winter hardy tree species under natural conditions, lending support to the hypothesis that FA-regulated PM H+-ATPase activity is involved in the cellular response underlying cold acclimation and de-acclimation.

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In vivo activation of plasma membrane H⁺‐ATPase hydrolytic activity by complex lipid‐bound unsaturated fatty acids in Ustilago maydis

Cited by 13 publications

References 42 publications

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots

Response of plasma membrane H+-ATPase to heavy metal stress in Cucumis sativus roots

Plant Plasma Membrane H+-ATPase in Adaptation of Plants to Abiotic Stresses

Changes in freezing tolerance, plasma membrane H+-ATPase activity and fatty acid composition in Pinus resinosa needles during cold acclimation and de-acclimation

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