<i>In vivo</i> administration of gut bacterial consortia replicates urolithin metabotypes A and B in a non-urolithin-producing rat model

Iglesias‐Aguirre, Carlos E.; González‐Sarrías, Antonio; Cortés‐Martín, Adrián; Romo‐Vaquero, María; Osuna-Galisteo, Leire; Cerón, José J.; Espı́n, Juan Carlos; Selma, María V.

doi:10.1039/d2fo03957e

Cited by 11 publications

(7 citation statements)

References 36 publications

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“…Recently, Iglesias-Aguirre et al reported two co-cultures able to convert ellagic acids into urolithins reproducing urolithin metabotypes A and B using only two gut bacteria per co-culture and the administration of these bacterial consortia successfully replicated urolithin metabotypes in rat. , Hence, new probiotic combinations could be formulated to engineer the gut microbiota of individuals unable to produce bioactive urolithins (urolithins metabotype 0) into urolithins metabotype A or B. Similar studies should be conducted to identify a bacterial consortium able to convert (−)-epicatechin, or more generally flavan-3-ols, into 3,4-DHPVL.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Interindividual Variability Associated with the Microbial Metabolism of (−)-Epicatechin

Lessard-Lord,

Roussel,

Guay

et al. 2023

J. Agric. Food Chem.

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Although the relationship between gut microbiota and flavan-3-ol metabolism differs greatly between individuals, the specific metabolic profiles, known as metabotypes, have not yet been clearly defined. In this study, fecal batch fermentations of 34 healthy donors inoculated with (−)-epicatechin were stratified into groups based on their conversion rate of (−)-epicatechin and their quali–quantitative metabolic profile. Fast and slow converters of (−)-epicatechin, high producers of 1-(3′-hydroxyphenyl)-3-(2″,4″,6″-trihydroxyphenyl)-propan-2-ol (3-HPP-2-ol) and 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone (3,4-DHPVL) were identified. Fecal microbiota analysis revealed that fast conversion of (−)-epicatechin was associated with short-chain fatty acid (SCFA)-producing bacteria, such as Faecalibacterium spp. and Bacteroides spp., and higher levels of acetate, propionate, butyrate, and valerate were observed for fast converters. Other bacteria were associated with the conversion of 1-(3′,4′-dihydroxyphenyl)-3-(2″,4″,6″-trihydroxyphenyl)-propan-2-ol into 3-HPP-2-ol (Lachnospiraceae UCG-010 spp.) and 3,4-DHPVL (Adlercreutzia equolifaciens). Such stratification sheds light on the mechanisms of action underlying the high interindividual variability associated with the health benefits of flavan-3-ols.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Interindividual Variability Associated with the Microbial Metabolism of (−)-Epicatechin

Lessard-Lord,

Roussel,

Guay

et al. 2023

J. Agric. Food Chem.

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“…Members of the Enterocloster spp. have previously been shown to metabolize (dehydroxylate) uroC in vitro 36 and in vivo 37 under anaerobic conditions (Fig. 1A, full metabolic pathway in Extended Data Fig.…”

Section: A Subset Of Enterocloster Species Converts Urolithin C To Ur...mentioning

confidence: 95%

“…Recently, certain members of the Enterocloster spp. (Lachnospiraceae family) were reported to dehydroxylate uroC to uroA and isouroA to urolithin B (uroB) both in vitro and in vivo 38,39 .…”

Section: Mainmentioning

confidence: 99%

The dietary ellagitannin metabolite urolithin A is produced by a molybdenum-dependent dehydroxylase encoded by prevalent human gutEnteroclosterspp

Pidgeon,

Mitchell,

Shamash

et al. 2024

Preprint

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Urolithin A is a polyphenol derived from the multi-step metabolism of dietary ellagitannins by the human gut microbiota that can affect host health. Most, but not all, individuals harbor a microbiota capable of urolithin A production; however, the enzymes that dehydroxylate its dietary precursor (urolithin C) are unknown. Here, we use a combination of untargeted transcriptomics, proteomics, and comparative genomics to uncover an inducible urolithin C dehydroxylase (ucd) operon in Lachnospiraceae. Biochemical characterization and structural modeling of proteins encoded by ucd demonstrated that urolithin C dehydroxylation was NADH-dependent, required strict anaerobic conditions, and used urolithin C as an electron acceptor. By combining functional assays in complex fecal communities with publicly available metagenomic data, our study reveals that both urolithin C-metabolizing bacteria and ucd operon genes are widely distributed in gut metagenomes and likely comprise keystone species in the metabolism of urolithins by the human gut microbiota.

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“…The potential to modulate UM is therefore worthy to explore. Recently, Iglesias-Aguirre et al 151 conducted an in vivo animal study to transfer urolithin-producing bacterial consortia with the aim to convert UM0 to UMA and UMB. Urolithin-producing bacterial strains, Gordonibacter and Ellagibacter , successfully colonised the rats’ gut and replicated the ability to produce urolithins.…”

Section: Introductionmentioning

confidence: 99%

(Poly)phenol-related gut metabotypes and human health: an update

Hu,

Mesnage,

Tuohy

et al. 2024

Food Funct.

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In vivo administration of gut bacterial consortia replicates urolithin metabotypes A and B in a non-urolithin-producing rat model

Abstract: Urolithins (Uros) production capacity and, consequently, at least partly, the health effects attributed to ellagitannins and ellagic acid consumption vary among individuals. The reason is that not all individuals have...

Cited by 11 publications

References 36 publications

Characterization of the Interindividual Variability Associated with the Microbial Metabolism of (−)-Epicatechin

Characterization of the Interindividual Variability Associated with the Microbial Metabolism of (−)-Epicatechin

The dietary ellagitannin metabolite urolithin A is produced by a molybdenum-dependent dehydroxylase encoded by prevalent human gutEnteroclosterspp

(Poly)phenol-related gut metabotypes and human health: an update

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