In vivo and in vitro Effects of Cadmium on Adult Rat Brain Total Antioxidant Status, Acetylcholinesterase, (Na+,K+)‐ATPase and Mg2+‐ATPase Activities: Protection by L‐Cysteine

Carageorgiou, Haris; Tzotzes, Vassilios; Pantos, Constantinos; Mourouzis, Constantinos; Ζάρρος, Απόστολος; Tsakiris, Stylianos

doi:10.1111/j.1742-7843.2004.pto940303.x

Cited by 100 publications

(61 citation statements)

References 37 publications

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“…Moreover, the enhancement of Ca 2+ /Mg 2+ activity may represent a rise in energy expenditure and metabolism of the organism because of the increased rate of ATP hydrolysis during oxidative phosphorylation and therefore respiration (Ulrich 1963). In line with previous studies (Cotou et al 2001;Carageorgiou et al 2004), the increased Ca 2+ -Mg 2+ -ATPase activity in our study may represent a compensatory physiological mechanism operating to maintain haemolymph ion concentrations and osmoregulatory function in fish exposed to TBT.…”

Section: Discussionsupporting

confidence: 92%

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin

Li¹,

Zhong²,

Mu³

et al. 2016

CZECH J ANIM SCI

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ABSTRACT:The effect of long-term exposure to tributyltin (TBT) on energy metabolism and adenosine triphosphatase (ATPase) enzymatic system in freshwater teleost was investigated. The impact on the parameters of energy metabolism (the content of glucose (Glu) and lactate (LA), and the activities of hexokinase (HK), pyruvate kinase (PK), and lactate dehydrogenase(LDH)) in the muscle tissue, as well as on the ATP enzymatic system (ATP content, Total-ATPase, Na -ATPase) in the gill tissue of common carp was evaluated. Fish were exposed to sublethal concentrations of TBT (75, 0.75, and 7.5 µg/l) for 60 days. Based on the results, long-term exposure to TBT could lead to obvious ATP enzymatic system responses, including the decreased ATP content and Na + -K + -ATPase activity, and the increased activities of Total-ATPase and Ca 2+ -Mg 2+ -ATPase. Moreover, the parameters of energy metabolism in muscle were also regulated, such as induced indices of the levels of Glu and LA, and the activities of HK and PK, and inhibited indices of LDH activity. Shortly, the measured physiological responses in fish could provide useful information to better understand the mechanisms of TBTinduced bio-toxicity, and could be used as potential biomarkers for monitoring the TBT pollution in the field.

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Section: Discussionsupporting

confidence: 92%

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin

Li¹,

Zhong²,

Mu³

et al. 2016

CZECH J ANIM SCI

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“…ATPases are membrane-bound enzymatic proteins which regulate ionic concentrations and it is vital for the normal functioning of the cells. These enzymes are sensitive to the alterations in the membrane lipid composition and oxidative stress (Carageorgiou et al 2004). In the present study, a significant decrease in Na (Jabs et al 1989;Schwartzman et al 1985;Lear et al 1990;Therien and Blostein 2000).…”

Section: Discussionmentioning

confidence: 55%

Fish oil rich in eicosapentaenoic acid protects against oxidative stress-related renal dysfunction induced by TCDD in Wistar rats

Palaniswamy

Vishwanadha

Singaravelu

2014

Cell Stress and Chaperones

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Humans are systemically exposed to persistent organic pollutants, of which 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) has become a major environmental concern. Exposure to TCDD results in a wide variety of adverse health effects which is mediated by oxidative stress through CYP1A1 activation and arachidonic acid metabolites. Eicosapentaenoic acid (EPA) exhibits antioxidant property and competes with arachidonic acid in membrane phospholipids and produces anti-inflammatory EPA derivatives. Since both EPA and its derivatives have been reported to enhance the antioxidant mechanism, the present study aimed at studying whether EPA could offer protection against TCDDinduced oxidative stress and nephrotoxicity in Wistar rats. Estimation of kidney markers (serum urea and creatinine) and histopathological studies revealed that EPA treatment significantly reduced TCDD-induced renal damage. TCDDinduced oxidative damage was reflected in a significant increase in CYP1A1 activity and lipid peroxide levels with a concomitant decline in non-enzymic antioxidant (GSH) and various enzymic antioxidants such catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), and glutathione peroxidase (GPx). In addition, TCDD-induced oxidative stress also resulted in decline in Na + -K + and Mg 2+ATPases activities with increase in Ca 2+ ATPases activity. Oral treatment with EPA showed a significant cytoprotection against TCDD-induced renal oxidative stress by decreased CYP1A1 activity and enhanced antioxidant status. TCDD-induced alterations in ATPase enzyme activities were also prevented by EPA treatment. Our results show clear evidence that EPA ameliorates TCDD-induced oxidative stress and kidney damage; thus suggest the potential of EPA as an effective therapeutic agent against toxic effects mediated through redox imbalance.

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“…Moreover, because  -cysteine (Cys) is a well-known antioxidant and chelating agent [22], it was co-administered with Mn in order to evaluate its efficacy on protecting the rat brain against the Mn-induced toxic effects on the above parameters.…”

Section: +mentioning

confidence: 99%

Effects of Short‐Term Exposure to Manganese on the Adult Rat Brain Antioxidant Status and the Activities of Acetylcholinesterase, (Na⁺,K⁺)‐ATPase and Mg²⁺‐ATPase: Modulation by l‐Cysteine

Liapi¹,

Ζάρρος²,

Galanopoulou³

et al. 2008

Basic Clin Pharma Tox

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Manganese (Mn) is an essential metalloenzyme component that in high doses can exert serious oxidative and neurotoxic effects. The aim of this study was to investigate the potential effect of the antioxidant  -cysteine (Cys, 7 mg/kg) on the adult rat brain total antioxidant status (TAS) and the activities of acetylcholinesterase (AChE), Na + ,K + -ATPase and Mg 2+ -ATPase induced by short-term Mn administration (as Mn chloride, 50 mg/kg). Twenty-eight male Wistar rats were divided into four groups: A (saline-treated control), B (Mn), C (Cys) and D (Mn and Cys). All rats were treated once daily, for 1 week with intraperitoneal injections of the tested compounds. Rats were killed by decapitation and mentioned parameters were measured spectrophotometrically. Rats treated with Mn exhibited a significant reduction in brain TAS ( − 39%, P < 0.001, B versus A) that was partially reversed by Cys co-administration ( − 13%, P < 0.01, D versus A), while Cys (group C) had no effect on TAS. The rat brain AChE activity was found significantly increased by both Mn (+21%, P < 0.001, B versus A) and Cys (+61%, P < 0.001, C versus A), while it was adjusted into the control levels by the co-administration of Mn and Cys. The activity of rat brain Na

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In vivo and in vitro Effects of Cadmium on Adult Rat Brain Total Antioxidant Status, Acetylcholinesterase, (Na⁺,K⁺)‐ATPase and Mg²⁺‐ATPase Activities: Protection by L‐Cysteine

Cited by 100 publications

References 37 publications

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin

Fish oil rich in eicosapentaenoic acid protects against oxidative stress-related renal dysfunction induced by TCDD in Wistar rats

Effects of Short‐Term Exposure to Manganese on the Adult Rat Brain Antioxidant Status and the Activities of Acetylcholinesterase, (Na⁺,K⁺)‐ATPase and Mg²⁺‐ATPase: Modulation by l‐Cysteine

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In vivo and in vitro Effects of Cadmium on Adult Rat Brain Total Antioxidant Status, Acetylcholinesterase, (Na+,K+)‐ATPase and Mg2+‐ATPase Activities: Protection by L‐Cysteine

Cited by 100 publications

References 37 publications

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin

Alteration of parameters of energy metabolism and ATPase enzymatic system in juvenile common carp (Cyprinus carpio) chronically exposed to tributyltin

Fish oil rich in eicosapentaenoic acid protects against oxidative stress-related renal dysfunction induced by TCDD in Wistar rats

Effects of Short‐Term Exposure to Manganese on the Adult Rat Brain Antioxidant Status and the Activities of Acetylcholinesterase, (Na+,K+)‐ATPase and Mg2+‐ATPase: Modulation by l‐Cysteine

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In vivo and in vitro Effects of Cadmium on Adult Rat Brain Total Antioxidant Status, Acetylcholinesterase, (Na⁺,K⁺)‐ATPase and Mg²⁺‐ATPase Activities: Protection by L‐Cysteine

Effects of Short‐Term Exposure to Manganese on the Adult Rat Brain Antioxidant Status and the Activities of Acetylcholinesterase, (Na⁺,K⁺)‐ATPase and Mg²⁺‐ATPase: Modulation by l‐Cysteine