In vivo
 and in vitro
 protein digestibility in juvenile bagrid catfish Mystus nemurus
 (Cuvier and Valenciennes 1840) fed soybean meal-based diets

Rahmah, Sharifah; Aliyu‐Paiko, Mohammed; Hashim, Roshada

doi:10.1111/are.12595

Cited by 9 publications

(9 citation statements)

References 41 publications

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“…and African catfish Clarias gariepinus (Abidin, Hashim, & Chien, 2006). This owes to its excellent taste and flesh quality (Rahmah, Aliyu‐Paiko, & Hashim, 2016), which contains comparatively higher polyunsaturated fatty acids (PUFA) than sardine (Mesomya et al., 2002). Bagrid catfish is carnivorous, requiring 60% dietary protein for larvae (Eguia, Kamarudin, & Santiago, 2000), 44% for juveniles (Ng, Soon, & Hashim, 2001) and 35% for female broodstock (Abidin et al., 2006).…”

Section: Introductionmentioning

confidence: 99%

Digestive proteases and in vitro protein digestibility in bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840)

2020

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Digestive proteases in the stomach and intestine of bagrid catfish Mystus nemurus juveniles and in vitro protein digestibility of fishmeal (FM), soya bean meal (SBM) and squid meal (SM) were determined. Postprandial data demonstrated highest protease activity 2 and 5 hr in the stomach and intestine, respectively, after feeding. Acidic-like protease in the stomach exhibited an optimum activity at pH 2, while alkaline proteases dominated the intestine, with optimum activities between pH 9 and 11. Investigations using specific commercial inhibitors confirmed the presence of pepsin in the stomach. Chymotrypsin, trypsin and metalloproteases were present in the intestine; however, the results indicated a possible involvement of other serine proteases. The highest inhibition of intestinal protease activity of bagrid catfish occurred during incubation with SM, followed by SBM and FM. Fishmeal had the highest relative protein digestibility (RPD), compared with SBM and SM, regardless of enzyme system used. Crude enzyme extract and the 4E system resulted in the highest RPD followed by 3E and 1E systems. The current results suggest that bagrid catfish adopt the typical protein digestion pathway of a carnivorous fish of endoprotease and exoprotease activities from the stomach to the intestine. Pepsin acts in the stomach, while trypsin, chymotrypsin, metalloproteases and other serine proteases are involved in the intestine. This study also recommends the use of crude intestinal enzyme extract for digestibiliy studies, instead of commercial enzyme systems, to better identify the suitable protein sources for bagrid catfish.

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Section: Introductionmentioning

confidence: 99%

Digestive proteases and in vitro protein digestibility in bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840)

2020

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“…However, there is a lack of a suitable formulated diet for this species so far (Deng et al, ; Hung, Binh, Thanh Truc, Tham, & Ngoc Tran, ). Furthermore, limited information is available on the utilization of plant protein sources in H. wyckioides (Phommanivong & Doolchidachabaporn, ; Rahmah, Aliyu‐Paiko, & Hashim, ). The objective of this study was to determine the effects of replacing FM with SBM on growth performance, feed utilization, digestive enzyme activity, protein metabolism and antioxidant capacity of juvenile H. wyckioides and thereby to evaluate the amount of SBM that could replace FM in the catfish diets.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of soybean meal as alternative to fish meal in diet for juvenile Asian red‐tailed catfish (Hemibagrus wyckioides)

Zhang

Wang

et al. 2019

Aquacult Nutr

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A feeding trial was conducted to determine the amount of soybean meal (SBM) that could replace fish meal (FM) without compromising growth and health of Asian red‐tailed catfish (Hemibagrus wyckioides). Five isonitrogenous and isoenergetic diets (S0, S15, S30, S45 and S60) were formulated with SBM to replace 0%, 15%, 30%, 45% and 60% of FM. The replacement level up to 30% improved daily growth coefficient, plasma adenosine monophosphate deaminase (AMPD) and alanine aminotransferase (ALT) activities and IgM content, and hepatic ALT, aspartate aminotransferase (AST) and glutathione reductase activities, whereas these were depressed by a further inclusion. The highest protein efficiency ratio and lowest feed conversion ratio were observed in fish fed the S15 diet. Replacement of FM with SBM generally decreased plasma insulin and insulin‐like growth factor 1 (IGF‐1) contents and hepatic catalase activity, whereas no significant differences were observed among fish fed the S0, S15 and S30 diets. In contrast, replacing FM with SBM generally increased blood urea nitrogen content, and that was higher in fish fed the S60 diet compared to fish fed the S0 diet. The highest growth hormone (GH) and glutamate dehydrogenase (GDH) activities were observed in fish fed the S30 diet. Fish fed the S30 and S45 diets exhibited the highest hepatic AMPD, GDH, IGF‐1 and target of rapamycin mRNA levels and muscle AMPD and GDH mRNA levels, whereas those were lowest in fish fed the S60 diet. These results indicate that under the reported conditions SBM may be included in the diet up to 222 g/kg as a substitute for FM, replacing about 30% of FM protein in juvenile H. wyckioides.

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“…Because protein is the most expensive and difficult ingredient to be replaced, research on developing fish meal replacement, using the in-vitro method, primarily targeted for carnivorous fish. Several other fish species have also been studied based on their local economic importance [7,16,[83][84][85].…”

Section: Current In-vitro Digestion Studies On Fishmentioning

confidence: 99%

“…By using an in-vitro digestion model, several studies have evaluated the effect of inhibitors and phytic acid on the digestibility of soybean to be utilized as a fish meal substitute [86][87][88]. Substitution of fish meal with soybean formulated as a fish feed had been studied on several different levels ranging from none to total replacement [83,84].…”

Section: Current In-vitro Digestion Studies On Fishmentioning

confidence: 99%

“…Several studies compared the crude extract of fish digestive enzymes with mammalian enzymes, showing proteases from fish crude extract have higher degree of hydrolysis compared to mammalian enzyme [ 20 , 94 , 95 ]. However, some studies showed contradictory results [ 84 , 85 , 96 ]. There are several reasons for the inconsistency as pointed out by Moyano et al [ 20 ], including substrates affinity, enzyme kinetics, optimum temperature and sensitivity to inhibitors.…”

Section: Application Of In-vitro Digestion Model I...mentioning

confidence: 99%

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In-vitro digestion models: a critical review for human and fish and a protocol for in-vitro digestion in fish

et al. 2021

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Digestive systems in human, animals, and fish are biological reactors and membranes to digest food and extract nutrients. Therefore, static and dynamic models of in-vitro digestion systems are developed to study e.g. novel food and feed before in-vivo studies. Such models are well developed for human, but not to the same extent for animals and fish. On the other hand, recent advances in aquaculture nutrition have created several potential fish meal replacements, and the assessment of their nutrient digestibility is critical in the application as a fish meal replacement. Using an in-vitro method, the assessment of an ingredient digestibility could be faster and less expensive compared to using an in-vivo experiment. An in-vitro method has been widely used to assess food nutrient digestibility for humans; however, its application for fish is still in the early stages. Both the human and fish as monogastric vertebrates share similar gastrointestinal systems; thus, the concept from the application for humans could be applied for fish. This review aims to improve the in-vitro digestion protocol for fish by adapting the concept from then study for humans, summarizing the current available in-vitro digestion model developed for human and fish in-vitro digestion study, identifying challenges specifically for fish required to be tackled and suggesting an engineering approach to adapt the human in-vitro gastrointestinal model to fish. Protocols to conduct in-vitro digestion study for fish are then proposed.

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In vivo and in vitro protein digestibility in juvenile bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840) fed soybean meal-based diets

Cited by 9 publications

References 41 publications

Digestive proteases and in vitro protein digestibility in bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840)

Digestive proteases and in vitro protein digestibility in bagrid catfish Mystus nemurus (Cuvier and Valenciennes 1840)

Evaluation of soybean meal as alternative to fish meal in diet for juvenile Asian red‐tailed catfish (Hemibagrus wyckioides)

In-vitro digestion models: a critical review for human and fish and a protocol for in-vitro digestion in fish

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