<i>In Vivo</i> and <i>In Vitro</i> Characterization of Mesothelial Lipid Inclusions

Jt, Hjelle; Bt, Golinska; Dc, Waters; Kr, Steidley; Dr, McCarroll; Pavlina, Thomas M.; Smith, Steven R.; Jk, Lloyd; Jw, Dobbie

doi:10.1177/089686089101100304

Cited by 11 publications

(2 citation statements)

References 10 publications

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“…This led Dobbie et al [2] to point out the similarity of composition to that of pulmonary surfactant, in which the major constituent is also phosphatidylcholine. It was shown that, in peritoneum, mesothelial cells are the source of these phospholipids in i o [3] and in itro [4] and that, similarly to alveolar type II cells, they display lamellar bodies in their cytoplasm and at their surface. Lamellar bodies have been shown in the lung to represent the storage and exocytosis form of surfactant.…”

Section: Introductionmentioning

confidence: 99%

Expression of hydrophilic surfactant proteins by mesentery cells in rat and man

Chailley‐Heu

Rubio

Rougier

et al. 1997

Biochemical Journal

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Human peritoneal dialysis effluent (PDE) contains a phosphatidylcholine-rich compound similar to the surfactant that lines lung alveoli. This material is secreted by mesothelial cells. Lung surfactant is also characterized by four proteins essential to its function. After having long been considered as lung-specific, some of them have been found in gastric and intestinal epithelial cells. To explore further the similarity between lung and peritoneal surfactants, we investigated whether mesothelial cells also produce surfactant proteins. We used rat transparent mesentery, human visceral peritoneum biopsies and PDE. Surfactant proteins were searched for after one- and two-dimensional SDS/PAGE and Western blotting. On a one-dimensional Western blot, bands at 38 and 66 kDa in rat mesentery, and at 38 and 66 kDa in human peritoneal mesothelial cells (in vivo and in vitro) and PDE, corresponded to monomeric and dimeric forms of lung surfactant protein A (SP-A). On two-dimensional Western blots, the 32 and 38 kDa spots in mesentery and PDE localized at the acidic pH appropriate to the SP-A monomer's isoelectric point. SP-D was also identified at the same 43 kDa molecular mass as in lung. SP-B was not detected in mesenteric samples. Expression of SP mRNA species was also assessed by reverse transcriptase-PCR, which was performed with specific primers of surfactant protein cDNA sequences. With primers of SP-A and SP-D, DNA fragments of the same size were amplified in lung and mesentery, indicating the presence of SP-A and SP-D mRNA species. These fragments were labelled by appropriate probes in a Southern blot. No amplification was obtained for SP-B. These results show that mesentery cells produce SP-A and SP-D, although they are of embryonic origin (mesodermal) and are different from those of the lung and digestive tract (endodermal) that secrete these surfactants.

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Section: Introductionmentioning

confidence: 99%

Expression of hydrophilic surfactant proteins by mesentery cells in rat and man

Chailley‐Heu

Rubio

Rougier

et al. 1997

Biochemical Journal

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“…Mesothelial cells have a remarkable biosynthetic potential, illustrated by the production of numerous micropinocytotic vesicles that form larger fused vesicles and vacuoles (Baradi and Rao, 1976;Ettarh and Carr, 1996). The cells synthesize and actively extrude a phospholipid-rich surfactant that lubricates the internal organs (Di Paolo, 1989;Dobbie and Lloyd, 1989;Hills, 2000;Hjelle et al, 1991). Mesothelial cells also reduce friction by the expression of numerous microvilli that mobilize and facilitate surfactant distribution, from surfactant-filled lamellar bodies (Andrews and Porter, 1973).…”

Section: Serosal Membrane Structure and Functionmentioning

confidence: 99%

Mesothelial primary cilia of peritoneal and other serosal surfaces

Bird

2004

Cell Biology International

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The conspicuous presence of primary cilia, a small immotile cilium present on most cell types, left researchers with little doubt of their functional relevance. Recently mechanosensitive functional significance was established and a link with the pathogenesis of polycystic kidney disease. Together these discoveries have raised the profile of this, previously considered "vestigial", organelle. Primary cilia are expressed on the apical surface of serosal mesothelium and display regional variation but are more abundant on biosynthetically active cells. Adult mesothelial cells are highly biosynthetic producing a phospholipid rich surfactant that lubricates and protects the visceral organs. The mesothelium is utilized as a semipermeable membrane during peritoneal dialysis for patients with end stage renal failure. However, little is known about the functional role of primary cilia on this highly specialized cell type. The present review, examines the significance of the primary cilium in serosal mesothelial cell biology with an emphasis on ciliary location, structure, form and function. Future research is identified and discussed in view of the emerging role cilia have in other cells and the established function of the serosal mesothelium in development, normal function, peritoneal dialysis and pathology of the serosal membranes.

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Mesothelial lamellar bodies in norm and experimental conditions. Transmission and scanning electron microscopic observations on the peritoneum, pleura and pericardium

Michailova

2004

Anat Embryol

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The ultrastructural characteristics of the mesothelial intracellular and extracellular lamellar bodies (LB) in norm, and especially in pathological conditions are still unknown. After routine fixation procedure, material from organs in the three serous cavities of Wistar rats, as control group were compared with animals following experimental hemothorax (EH) and experimental peritonitis (EP), using transmission and scanning electron microscopy (TEM, SEM). Different membrane-bound profiles, short strip-like structures and single LB characterize the control group. Five days after EH small groups of LB were observed. Single balloon-like profiles, numerous particles and larger groups of LB with wide varieties in size, form and membrane structure characterize the 8th day after EH. Thin and concentric membranes build single LB the 5th day of EP. Eight days after the same treatment, groups of LB, complex structures with several multilamellar centers, strip-like structures with rough granulo-filamentous material and uninterrupted covering over wide areas were observed. We conclude that constant components in the untreated rats are different membrane profiles, as initial lamellar formations and single typical LB with predominant intracellular position. The preferable sites for exocytosis of LB are the intercellular spaces. The present data demonstrate simultaneous findings of the two main groups of formations by using routine fixation in the control group and in the treated animals. The first represent LB as well as other round profiles, observed by TEM and particles, observed by SEM, which are widespread and probably have a basic role. The second ones are strip-like structures and its SEM-equivalents, i.e. uninterrupted covering. TEM-profiles and their SEM-images show significant organ differences and in some cases lack of correspondence between both electron microscopic techniques in the same treatment. Significant enlargement of the number of LB and the length of the strip-like structures characterize the mesothelium after pathologic conditions. LB with considerable differences in the structure and larger particles over the microvillous border predominate after EH. Strip-like profiles, continuous covering and smaller groups of LB with similar morphology and preferable submesothelial position characterize the mesothelium after EP.

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In Vivo and In Vitro Characterization of Mesothelial Lipid Inclusions

Cited by 11 publications

References 10 publications

Expression of hydrophilic surfactant proteins by mesentery cells in rat and man

Expression of hydrophilic surfactant proteins by mesentery cells in rat and man

Mesothelial primary cilia of peritoneal and other serosal surfaces

Mesothelial lamellar bodies in norm and experimental conditions. Transmission and scanning electron microscopic observations on the peritoneum, pleura and pericardium

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