In vivo and in vitro immunomodulation of Der p 1 allergen‐specific response by Lactobacillus plantarum bacteria

Hisbergues, Michael; Magi, Mauro; Rigaux, Peter; Steuve, Jonathan; Garcia, Lida; Goudercourt, Denise; Pot, Bruno; Pestel, Joël; Jacquet, Alain

doi:10.1111/j.1365-2222.2007.02792.x

Cited by 66 publications

(60 citation statements)

References 47 publications

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“…TLR2 and TLR4 are expressed at the cell surface, while TLR9 is expressed in endosomes (34). Recently, the specific TLRs involved in LAB recognition and cytokine production by DCs and macrophages have been identified (35)(36)(37)(38). Rigaux et al have reported that IL-12p40 secretion by mouse DCs in response to L. plantarum NCIMB8826 was dependent on TLR2-and TLR9-mediated signaling (37).…”

Section: Discussionmentioning

confidence: 99%

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Kawashima

Murakami²,

Nishimura³

et al. 2011

Int J Mol Med

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Abstract. Fucoidan, a sulfated polysaccharide contained in brown algae, has a variety of immunomodulatory effects, including antitumor and antiviral effects. On the other hand, lactic acid bacteria (LAB) also have immunomodulatory effects such as anti-allergic effects. In this study, we demonstrated that fucoidan enhances the probiotic effects of LAB on immune functions. By using Peyer's patch cells and spleen cells in vitro, fucoidan amplified interferon (IFN)-γ production in response to a strain of LAB, Tetragenococcus halophilus KK221, and this activity was abolished by desulfation of fucoidan. Moreover, this IFN-γ response was abolished by interleukin (IL)-12 neutralization. These results indicate that fucoidan enhanced IL-12 production in response to KK221, resulting in promoting IFN-γ production. In an in vivo study, Th1/Th2 immunobalance was most improved by oral administration of both fucoidan and KK221 to ovalbumin-immunized mice. These findings suggest that fucoidan can enhance a variety of beneficial effects of LAB on immune functions.

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Section: Discussionmentioning

confidence: 99%

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Kawashima

Murakami²,

Nishimura³

et al. 2011

Int J Mol Med

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“…42,43 This modulation occurs either by the promotion of Th1-type responses 44 or by the induction of regulatory cells and cytokines. 20,45 Using a gnotobiotic mouse model, we showed that colonization with Lmix reduced the serum levels of the Th2-related Bet v 1-specific IgE and IgG1 antibodies as well as the Th1-related IgG2a antibody, implicating the involvement of regulatory mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

et al. 2015

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Increasing numbers of clinical trials and animal experiments have shown that probiotic bacteria are promising tools for allergy prevention. Here, we analyzed the immunomodulatory properties of three selected lactobacillus strains and the impact of their mixture on allergic sensitization to Bet v 1 using a gnotobiotic mouse model. We showed that Lactobacillus (L.) rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 are recognized via Toll-like receptor 2 (TLR2) and nucleotide-binding oligomerization domain-containing protein 2 (NOD2) receptors and stimulate bone marrow-derived dendritic cells to produce cytokines in species-and strain-dependent manners. Colonization of germ-free (GF) mice with a mixture of all three strains (Lmix) improved the intestinal barrier by strengthening the apical junctional complexes of enterocytes and restoring the structures of microfilaments extending into the terminal web. Mice colonized with Lmix and sensitized to the Bet v 1 allergen showed significantly lower levels of allergen-specific IgE, IgG1 and IgG2a and an elevated total IgA level in the sera and intestinal lavages as well as an increased transforming growth factor (TGF)-b level compared with the sensitized GF mice. Splenocytes and mesenteric lymph node cells from the Lmix-colonized mice showed the significant upregulation of TGF-b after in vitro stimulation with Bet v 1. Our results show that Lmix colonization improved the gut epithelial barrier and reduced allergic sensitization to Bet v 1. Furthermore, these findings were accompanied by the increased production of circulating and secretory IgA and the regulatory cytokine TGF-b. Thus, this mixture of three lactobacillus strains shows potential for use in the prevention of increased gut permeability and the onset of allergies in humans. Cellular & Molecular Immunology

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“…Schiffrin et al [12] and Gill et al [13] evaluated the effects of two different probiotics in healthy adults, but these studies did not have a placebo group. Efforts trying to compare the in vitro results of one probiotic to its results in an in vivo setting are even more scarce and are at the moment limited to comparisons between in vitro and experimental animal studies [14][15][16] .…”

Section: Probiotic Intervention Has Strain-specific Anti-inflammatorymentioning

confidence: 99%

Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

Kekkonen

Lummela²,

Karjalainen³

et al. 2008

WJG

184

152

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freudenreichii ssp. shermanii JS (PJS) or a placebo drink for 3 wk. Venous blood and saliva samples were taken at baseline and on d 1, 7 and 21. Fecal samples were collected at baseline and at the end of intervention. RESULTS:The serum hsCRP expressed as the median AUC0-21 (minus baseline) was 0.018 mg/L in the placebo group, -0.240 mg/L in the LGG group, 0.090 mg/L in the Bb12 group and -0.085 mg/L in the PJS group (P = 0.014). In vitro production of TNF-α from in vitro cultured peripheral blood mononuclear cells (PBMC) was significantly lower in subjects receiving LGG vs placebo.IL-2 production from PBMC in the Bb12 group was significantly lower compared with the other groups. CONCLUSION:In conclusion, probiotic bacteria have strain-specific anti-inflammatory effects in healthy adults.

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In vivo and in vitro immunomodulation of Der p 1 allergen‐specific response by Lactobacillus plantarum bacteria

Cited by 66 publications

References 47 publications

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

A sulfated polysaccharide, fucoidan, enhances the immunomodulatory effects of lactic acid bacteria

Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

Probiotic intervention has strain-specific anti-inflammatory effects in healthy adults

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