<i>In vivo</i>and<i>in vitro</i>antiviral activity of five Tibetan medicinal plant extracts against herpes simplex virus type 2 infection

Zhang, Chunjiang; Li, Wei; Li, Hongyu; Wang, Yuling; Tian, Yun; Song, Zheng-Peng; Song, Yan; Zhao, Xingwen

doi:10.1080/13880200902905904

Cited by 16 publications

(7 citation statements)

References 26 publications

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“…This is in agreement with what was reported on Swertia mussoti, Dracocephalum heterophyllum, Dracocephalum tanguticum and Lagotis brevituba by Zhang and colleagues. 34 …”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

Hadun¹,

Mbaria²,

Aboge³

et al. 2018

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Introduction: At present, acyclovir is commercially available as the drug of choice for managing herpes simplex type I (HSV-1) viral infection. However, the high prevalence of the infection coupled with the emergence of resistant viral strains has limited its effectiveness. Thus, the development of novel antiviral agents is crucial. Practitioners of herbal medicine in Kenya make use of Dicrocephala integrifolia (DI) for the management of several diseases including viral infections. However, information on the efficacy of this plant against HSV-1 viral infection is not available. The aim of the present study was to determine the in vitro antiviral activity of crude extracts of DI against HSV-1. Methods: Leaves, roots, flowers and stems of DI were extracted using water (W) and methanol (ME) and qualitatively screened to identify the phytoconstituents present. Furthermore, the anti HSV-1 activity of the obtained extracts was evaluated on Vero cell lines using the 3-[4, 5 dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide] assay. The 50% cytotoxic concentration (CC 50 ) and 50% effective concentration (EC 50 ) of each extract was determined using regression analysis. The effects of crude DI extracts on adsorption and post-adsorption stages of the HSV-1 replication cycle was evaluated against acyclovir using a cytopathogenic inhibition assay. Results: Alkaloids, glycosides, flavonoids, phenols, saponins, tannins and terpenoids were found to be present in the extracts. The CC 50 values of the aqueous DI extracts was in the range 71.31 ± 2.65 to >100 µg/ml compared to >100 µg/ml of acyclovir. The EC 50 values of crude extracts of DI on the pre-adsorptive phase of HSV-1 activity was in the range 54.45±3.45 to >100µg/ml compared to 4.772±7.81µg/ml of acyclovir whilst the EC 50 value of the crude extracts of DI on the post-adsorptive phase of HSV-1 activity was in the range 45.270±4.31 to >100µg/ml compared to >100µg/ml of acyclovir. Conclusion: The results suggest that crude extracts of DI may be a reservoir of phytochemicals with potentially good efficacy against HSV-1.

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“…This is in agreement with what was reported on Swertia mussoti, Dracocephalum heterophyllum, Dracocephalum tanguticum and Lagotis brevituba by Zhang and colleagues. 34 …”

Section: Discussionmentioning

confidence: 99%

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

Hadun¹,

Mbaria²,

Aboge³

et al. 2018

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“…The most generally utilized strategy in many related studies is to evaluate the strength of small molecule binding by employing the binding free energy (BFE) approach [51][52][53][54]. We also adopted the MMPBSA.py script to compute the binding free energy of the proteinligand complexes by evaluating 2500 snapshots, keeping in mind the relevance of this strategy in re-ranking the binding conformations.…”

Section: Estimation Of Post-simulation Binding Energymentioning

confidence: 99%

Computational Investigations of Traditional Chinese Medicinal Compounds against the Omicron Variant of SARS-CoV-2 to Rescue the Host Immune System

et al. 2022

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Macrodomain-I of the NSP3 (non-structural protein 3) is responsible for immune response hijacking in the SARS-CoV-2 infection known as COVID-19. In the omicron variant (B.1.1.529), this domain harbors a new mutation, V1069I, which may increase the binding of ADPr and consequently the infection severity. This macrodomain-I, due to its significant role in infection, is deemed to be an important drug target. Hence, using structural bioinformatics and molecular simulation approaches, we performed a virtual screening of the traditional Chinese medicines (TCM) database for potential anti-viral drugs. The screening of 57,000 compounds yielded the 10 best compounds with docking scores better than the control ADPr. Among the top ten, the best three hits—TCM42798, with a docking score of −13.70 kcal/mol, TCM47007 of −13.25 kcal/mol, and TCM30675 of −12.49 kcal/mol—were chosen as the best hits. Structural dynamic features were explored including stability, compactness, flexibility, and hydrogen bonding, further demonstrating the anti-viral potential of these hits. Using the MM/GBSA approach, the total binding free energy for each complex was reported to be −69.78 kcal/mol, −50.11 kcal/mol, and −47.64 kcal/mol, respectively, which consequently reflect the stronger binding and inhibitory potential of these compounds. These agents might suppress NSP3 directly, allowing the host immune system to recuperate. The current study lays the groundwork for the development of new drugs to combat SARS-CoV-2 and its variants.

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“…The EA fraction (238.54 g) was separated by silica gel column chromatography (CC) using PE/EA (200 : 1-0 : 100) to obtain twelve fractions (Frs. [1][2][3][4][5][6][7][8][9][10][11][12]. Frs.…”

Section: Extraction and Isolationmentioning

confidence: 99%

“…has created unique vegetation types and species distribution in the region, thus creating a huge resource pool of natural precious medicinal herbs. [1,2] In recent years, Tibetan medicine has attracted increasing attention because of its extensive biological activities such as antioxidant, [3,4] anti-inflammatory, [5,6] antiviral [7] and antitumor. [8,9] Rhododendron nivale hook.f.…”

Section: Introductionmentioning

confidence: 99%

Sesquiterpenes fromRhododendron nivaleand Their Anti‐Inflammatory Activity

Yuan

Zeng

Liu

2022

Chemistry & Biodiversity

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Two new germacrane sesquiterpenes, named nivalenoids A and B (1 and 2), along with four known sesquiterpenes (3–6), were isolated from the Rhododendron nivale. The structures of compounds 1 and 2 were elucidated by integrating spectroscopic analyses, including NMR, UV, IR, as well as HR‐MS data. Anti‐inflammatory activity by inhibiting lipopolysaccharide (LPS)‐induced NO production in RAW 264.7 cells revealed that compared with the positive control dexamethasone (IC50=2.50 μM), compounds 1 and 2 showed mild anti‐inflammatory activity, with IC50 value of 27.55 μM and 53.26 μM, respectively.

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In vivoandin vitroantiviral activity of five Tibetan medicinal plant extracts against herpes simplex virus type 2 infection

Cited by 16 publications

References 26 publications

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

Computational Investigations of Traditional Chinese Medicinal Compounds against the Omicron Variant of SARS-CoV-2 to Rescue the Host Immune System

Sesquiterpenes fromRhododendron nivaleand Their Anti‐Inflammatory Activity

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