<i>In vivo</i> assessment of a nanofibrous silk tube as nerve guide for sciatic nerve regeneration

Ebrahimi, Maryam; Ai, Jafar; Biazar, Esmaeil; Ebrahimi‐Barough, Somayeh; Khojasteh, Arash; Yazdankhah, Meysam; Sharifi, Shahriar; Ai, Arman; Keshel, Saeed Heidari

doi:10.1080/21691401.2018.1426593

Cited by 18 publications

(17 citation statements)

References 49 publications

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“…The study reports successful results for bridging a 10 mm gap within the sciatic nerve [55]. The conceptual framework of this study was confirmed in a more recent study, and similar positive incomes were observed [99]. In line with these results, Gu et al assessed the ability of SF fibers with chitosan for providing a neuronal-like niche for culturing Schwann cells that were able to deposit ECM in the porosity of the biomaterial [152,153].…”

Section: The Importance Of Silk In Peripheral Nerve Injurysupporting

confidence: 75%

“…SF fibers can be gathered in various collectors to make 2D and 3D highly arranged mats or weaves of different shapes. This method has the potential to fabricate highly arranged structures to guide cells and push them toward further proliferation, differentiation, and adhesion and to be used as conduits for axonal guidance and neuronal regeneration [44,99,100]. A main issue is the hardship of constructing structured 3D scaffolds to fill spatial defects in the nervous tissue [101].…”

Section: Fabrication Of Fiber-based Biomaterialsmentioning

confidence: 99%

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Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

et al. 2021

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Silk refers to a family of natural fibers spun by several species of invertebrates such as spiders and silkworms. In particular, silkworm silk, the silk spun by Bombyx mori larvae, has been primarily used in the textile industry and in clinical settings as a main component of sutures for tissue repairing and wound ligation. The biocompatibility, remarkable mechanical performance, controllable degradation, and the possibility of producing silk-based materials in several formats, have laid the basic principles that have triggered and extended the use of this material in regenerative medicine. The field of neural soft tissue engineering is not an exception, as it has taken advantage of the properties of silk to promote neuronal growth and nerve guidance. In addition, silk has notable intrinsic properties and the by-products derived from its degradation show anti-inflammatory and antioxidant properties. Finally, this material can be employed for the controlled release of factors and drugs, as well as for the encapsulation and implantation of exogenous stem and progenitor cells with therapeutic capacity. In this article, we review the state of the art on manufacturing methodologies and properties of fiber-based and non-fiber-based formats, as well as the application of silk-based biomaterials to neuroprotect and regenerate the damaged nervous system. We review previous studies that strategically have used silk to enhance therapeutics dealing with highly prevalent central and peripheral disorders such as stroke, Alzheimer’s disease, Parkinson’s disease, and peripheral trauma. Finally, we discuss previous research focused on the modification of this biomaterial, through biofunctionalization techniques and/or the creation of novel composite formulations, that aim to transform silk, beyond its natural performance, into more efficient silk-based-polymers towards the clinical arena of neuroprotection and regeneration in nervous system diseases.

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Section: The Importance Of Silk In Peripheral Nerve Injurysupporting

confidence: 75%

Section: Fabrication Of Fiber-based Biomaterialsmentioning

confidence: 99%

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

et al. 2021

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“…Rehydration was performed with 100 to 70% graded alcohol, and the organic solution was removed by rinsing in ddH 2 O. Hematoxylin and eosin (HE) staining (Sigma-Aldrich), toluidine blue staining (Sigma-Aldrich) (Ebrahimi et al, 2018), myelin basic protein (MBP) immunostaining (Merolli et al, 2019) and iron staining (Sigma-Aldrich) (Liu and Ho, 2017) were used to stain the sections, with the results being examined by a light microscope AX80 (Olympus, Tokyo, Japan). Photographs of the toluidine blue staining sections taken under light microscopy at a 400 × magnification were used for quantification.…”

Section: Histological Stain and Myelinated Fiber Quantificationmentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cells From a Hypoxic Culture Improve Neuronal Differentiation and Nerve Repair

Liao

Hsueh

et al. 2021

Front. Cell Dev. Biol.

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Hypoxic expansion has been demonstrated to enhance in vitro neuronal differentiation of bone-marrow derived mesenchymal stem cells (BMSCs). Whether adipose-derived mesenchymal stem cells (ADSCs) increase their neuronal differentiation potential following hypoxic expansion has been examined in the study. Real-time quantitative reverse transcription-polymerase chain reaction and immunofluorescence staining were employed to detect the expression of neuronal markers and compare the differentiation efficiency of hypoxic and normoxic ADSCs. A sciatic nerve injury animal model was used to analyze the gastrocnemius muscle weights as the outcomes of hypoxic and normoxic ADSC treatments, and sections of the regenerated nerve fibers taken from the conduits were analyzed by histological staining and immunohistochemical staining. Comparisons of the treatment effects of ADSCs and BMSCs following hypoxic expansion were also conducted in vitro and in vivo. Hypoxic expansion prior to the differentiation procedure promoted the expression of the neuronal markers in ADSC differentiated neuron-like cells. Moreover, the conduit connecting the sciatic nerve gap injected with hypoxic ADSCs showed the highest recovery rate of the gastrocnemius muscle weights in the animal model, suggesting a conceivable treatment for hypoxic ADSCs. The percentages of the regenerated myelinated fibers from the hypoxic ADSCs detected by toluidine blue staining and myelin basic protein (MBP) immunostaining were higher than those of the normoxic ones. On the other hand, hypoxic expansion increased the neuronal differentiation potential of ADSCs compared with that of the hypoxic BMSCs in vitro. The outcomes of animals treated with hypoxic ADSCs and hypoxic BMSCs showed similar results, confirming that hypoxic expansion enhances the neuronal differentiation potential of ADSCs in vitro and improves in vivo therapeutic potential.

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“…NGCs are advantageous in that they reduce the ingrowth of scar tissue in the site of injury; contribute to the sprouting of axons from the proximal nerve; centralize growth factors secreted by the damaged nerve ends [37]. Currently, researchers have widely used natural or synthetic biomaterials to fabricate NGCs with various structural and physicochemical features ( Figure 2C-J) [38][39][40]. Tailoring NGC chemical structure and composition provide a possibility to obtain a conduit with desired properties such as excellent mechanical strength, good biocompatibility, biodegradability, and permeability.…”

Section: Nerve Tissue Engineering Strategiesmentioning

confidence: 99%

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

Amani

Kazerooni

Hassanpoor

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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The nervous system is known as a crucial part of the body and derangement in this system can cause potentially lethal consequences or serious side effects. Unfortunately, the nervous system is unable to rehabilitate damaged regions following seriously debilitating disorders such as stroke, spinal cord injury and brain trauma which, in turn, lead to the reduction of quality of life for the patient. Major challenges in restoring the damaged nervous system are low regenerative capacity and the complexity of physiology system. Synthetic polymeric biomaterials with outstanding properties such as excellent biocompatibility and non-immunogenicity find a wide range of applications in biomedical fields especially neural implants and nerve tissue engineering scaffolds. Despite these advancements, tailoring polymeric biomaterials for design of a desired scaffold is fundamental issue that needs tremendous attention to promote the therapeutic benefits and minimize adverse effects. This review aims to (i) describe the nervous system and related injuries. Then, (ii) nerve tissue engineering strategies are discussed and (iii) physiochemical properties of synthetic polymeric biomaterials systematically highlighted. Moreover, tailoring synthetic polymeric biomaterials for nerve tissue engineering is reviewed.

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In vivo assessment of a nanofibrous silk tube as nerve guide for sciatic nerve regeneration

Cited by 18 publications

References 49 publications

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

Silk Fibroin: An Ancient Material for Repairing the Injured Nervous System

Adipose-Derived Mesenchymal Stem Cells From a Hypoxic Culture Improve Neuronal Differentiation and Nerve Repair

Tailoring synthetic polymeric biomaterials towards nerve tissue engineering: a review

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