In Vivo Bioluminescence Imaging of Cobalt Accumulation in a Mouse Model

Abstract

As a trace element nutrient, cobalt is critical for both prokaryotes and eukaryotes. In the current study, a turn-on Cobalt Bioluminescent Probe 1 (CBP-1) for the detection of cobalt has been successfully developed based on oxidative C-O bond cleavage. This probe exhibited high selectivity and sensitivity toward cobalt over other analytes. By using CBP-1, the successful in vivo imaging of cobalt accumulation was carried out in a mouse model. Such an ability to determine cobalt in living animals provides a powerful technology for studying the system distribution, toxic potency, and biological effect of Co.

Aktivitätsbasierte Sensorik: ein synthetisch‐methodischer Ansatz für die selektive molekulare Bildgebung und darüber hinaus.

“…In einem Fall passten wir diesen Auslöser an und entwickelten so Copper Caged Luciferin‐1 (CCL‐1), eine Sonde, die die Ganztierabbildung von Kupferreservoirs in vivo ermöglicht. Unser Labor stellte auch die Cobaltsonde 1 (CP1) durch eine verwandte oxidative Spaltung her, indem ein N 3 O‐Rezeptor für die Co II ‐Bildgebung an lebenden Zellen verwendet wurde; diese wurde für ein Biolumineszenz‐Abbildungsverfahren weiter ausgearbeitet . Wir entwickelten auch eine Sonde der ersten Generation für die Darstellung von labilem Fe II , die Eisensonde 1 (IP1), die Veränderungen an den labilen Eisenvorkommen mit einem Trispyridincarboxylatrezeptor für Eisenaktivität sichtbar machen kann …”

Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy: In Vitro and In Vivo Perspectives

“…The presence of Fe 2+ caused the cleavage of conjugate and the release of D -aminoluciferin, which generated an increased BL signal that was correlated with the amount of Fe 2+ . Very recently, the accumulation of Co 2+ was also imaged via the split of luciferin-based conjugate 146. The Co 2+ BL probe 1 (CBP-1) was synthesized using the N 3 O ligand and a C-O benzyl ether bond, in which the tetradentate ligand N 3 O was the trigger for Co 2+ .…”

“…(B) Synthesis of CBP-1 and in vivo imaging of Co 2+ . Reprinted with permission from 146, Copyright 2018, American Chemical Society. (C) Synthesis of acrylic ester luciferin and in vivo imaging of Cys.…”

Caged luciferins for bioluminescent activity-based sensing

“…These findings correlated with transcriptional changes with elevations in iron acquisition and storage proteins in the infected tissues, suggesting a potential nutrient starvation mechanism by the host to protect against bacterial invasion. Based onOn the basis of fluorescent activity-based sensing probes [4,46], caged luciferins for sensing Co 2+ and Hg 2+ activity in vivo have been developed by Li and co-workers [47,48]. Taken together, the growing toolbox of bioluminescent activity-based sensing platforms for longitudinally assessing metal status in living animals provides a powerful technology for studying the contributions of metal metabolism and signaling to healthy and disease states.…”