2000
DOI: 10.1046/j.1529-8817.1999.00001-7.x
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IN VIVO CHARACTERIZATION OF THE DIATOM MULTIPARTATE PLASTID TARGETING SIGNAL

Abstract: Diatoms and related algae have plastids that are surrounded by four membranes. The outer two membranes are continuous with the endoplasmic reticulum and the inner two membranes are analogous to the plastid envelope membranes of higher plants and green algae. Thus the plastids are completely compartmentalized within the ER membranes. The targeting presequences for nuclear‐encoded plastid proteins have two recognizable domains. The first domain is a classic signal sequence, which presumably targets the proteins … Show more

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“…Recent developments allow gene function to be evaluated using RNAi suppression of gene activity, and GFP fusion proteins are available to elucidate localization of gene products. On the other hand, transformation experiments are also reported, such as those for C. merolae ) and several diatoms (Dunahey et al 1995;Apt et al 1996Apt et al , 2002Falciatore et al 1999). If functional analysis using gene disruption becomes possible by improving gene manipulation methods for these species, algae will become very useful in many research fields.…”
Section: Resultsmentioning
confidence: 96%
“…Recent developments allow gene function to be evaluated using RNAi suppression of gene activity, and GFP fusion proteins are available to elucidate localization of gene products. On the other hand, transformation experiments are also reported, such as those for C. merolae ) and several diatoms (Dunahey et al 1995;Apt et al 1996Apt et al , 2002Falciatore et al 1999). If functional analysis using gene disruption becomes possible by improving gene manipulation methods for these species, algae will become very useful in many research fields.…”
Section: Resultsmentioning
confidence: 96%