<i>In vivo</i> Confocal Raman Spectroscopic Analysis of the Effects of Infrared Radiation in the Human Skin Dermis

Lopes, Monica Bergamo; Rajasekaran, R.; Cançado, Ana Clara Figueira Lopes; Martin, Aírton Abrahão

doi:10.1111/php.12701

Cited by 11 publications

(7 citation statements)

References 54 publications

(79 reference statements)

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“…6) is placed at a distance of 0.7m from the seated subject. The heat lamp is placed at a distance of 0.5m [87], [88] from the subject with the lamp focused normally on the subject's face, to ensure uniform heating of all facial regions. The subject is instructed to perform left-right head rotation for the duration of the video recording, guided by 5 flags placed on the table at angles of 30°, 60°, 90°, 120°and 150°from left to right.…”

Section: Experimental Protocol To Develop the Nest-lab Datasetmentioning

confidence: 99%

Video-Based Elevated Skin Temperature Detection

Dasari

Revanur

Jeni

et al. 2023

IEEE Trans. Biomed. Eng.

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In this work, we propose a non-contact videobased approach that detects when an individual's skin temperature is elevated beyond the normal range. The detection of elevated skin temperature is critical as a diagnostic tool to infer the presence of an infection or an abnormal health condition. Detection of elevated skin temperature is typically achieved using contact thermometers or noncontact infrared-based sensors. The ubiquity of video data acquisition devices such as mobile phones and computers motivates the development of a binary classification approach, the Video-based TEMPerature (V-TEMP) to classify subjects with non-elevated/elevated skin temperature. We leverage the correlation between the skin temperature and the angular reflectance distribution of light, to empirically differentiate between skin at non-elevated temperature and skin at elevated temperature. We demonstrate the uniqueness of this correlation by 1) revealing the existence of a difference in the angular reflectance distribution of light from skin-like and non-skin like material and 2) exploring the consistency of the angular reflectance distribution of light in materials exhibiting optical properties similar to human skin. Finally, we demonstrate the robustness of V-TEMP by evaluating the efficacy of elevated skin temperature detection on subject videos recorded in 1) laboratory controlled environments and 2) outside-the-lab environments. V-TEMP is beneficial in two ways; (1) it is noncontact-based, reducing the possibility of infection due to contact and (2) it is scalable, given the ubiquity of videorecording devices.

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Section: Experimental Protocol To Develop the Nest-lab Datasetmentioning

confidence: 99%

Video-Based Elevated Skin Temperature Detection

Dasari

Revanur

Jeni

et al. 2023

IEEE Trans. Biomed. Eng.

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“…For skin analysis not related to cancer, but to characterize depth profiles of stratum corneum, e.g., penetration studies of drugs or cosmetic products, in numerous cases a model 3510 Skin Composition Analyzer (River Diagnostics, Rotterdam, The Netherlands) was frequently employed. [74][75][76][77][78] An alternative miniaturized, confocal fiber optic probe intended to fit within the instrument channel of a standard medical endoscope, with a diameter of 2.8 mm, was developed by Day et al 36 It was optimized for the study of the carcinogenesis process of esophageal malignancy. The optical layout is related to the probe shown in Fig.…”

Section: Basic Raman Probe Setupsmentioning

confidence: 99%

In-vivo Raman spectroscopy: from basics to applications

et al. 2018

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For more than two decades, Raman spectroscopy has found widespread use in biological and medical applications. The instrumentation and the statistical evaluation procedures have matured, enabling the lengthy transition from ex-vivo demonstration to in-vivo examinations. This transition goes hand-in-hand with many technological developments and tightly bound requirements for a successful implementation in a clinical environment, which are often difficult to assess for novice scientists in the field. This review outlines the required instrumentation and instrumentation parameters, designs, and developments of fiber optic probes for the in-vivo applications in a clinical setting. It aims at providing an overview of contemporary technology and clinical trials and attempts to identify future developments necessary to bring the emerging technology to the clinical end users. A comprehensive overview of in-vivo applications of fiber optic Raman probes to characterize different tissue and disease types is also given.

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“…In fact, experimentally observed spectral changes in fingerprint region for tissues have been qualitatively re-ported to correlated to water content (see,e.g., refs. [22][23][24][25]). Elderly and diabetis [22,23], oral cancer [24], cervical cancer [25] to name but a few are examples of physiological situations where spectral complexity emerges beyond the isolated molecule vibrational bands assignment.…”

Section: Viewmentioning

confidence: 99%

“…[22][23][24][25]). Elderly and diabetis [22,23], oral cancer [24], cervical cancer [25] to name but a few are examples of physiological situations where spectral complexity emerges beyond the isolated molecule vibrational bands assignment. Protein-water interactions are known to play a critical role in the function of several biological systems and macromolecules including collagen in tissues [26].…”

Section: Viewmentioning

confidence: 99%

First-principles calculations of Raman vibrational modes in the fingerprint region for connective tissue

Sato¹,

Martinho²

2018

Biomed. Opt. Express

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Vibrational spectroscopy has been widely employed to unravel the physical-chemical properties of biological systems. Due to its high sensitivity to monitoring real time "" changes, Raman spectroscopy has been successfully employed, e.g., in biomedicine, metabolomics, and biomedical engineering. The interpretation of Raman spectra in these cases is based on the isolated macromolecules constituent vibrational assignment. Due to this, probing the anharmonic or the mutual interactions among specific moieties/side chains is a challenge. We present a complete vibrational modes calculation for connective tissue in the fingerprint region (800 - 1800 cm) using first-principles density functional theory. Our calculations accounted for the inherent complexity of the spectral features of this region and useful spectral markers for biological processes were unambiguously identified. Our results indicated that important spectral features correlated to molecular characteristics have been ignored in the current tissue spectral bands assignments. In particular, we found that the presence of confined water is mainly responsible for the observed spectral complexity.

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In vivo Confocal Raman Spectroscopic Analysis of the Effects of Infrared Radiation in the Human Skin Dermis

Cited by 11 publications

References 54 publications

Video-Based Elevated Skin Temperature Detection

Video-Based Elevated Skin Temperature Detection

In-vivo Raman spectroscopy: from basics to applications

First-principles calculations of Raman vibrational modes in the fingerprint region for connective tissue

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