In vivo effects of human adipose-derived stem cells reseeding on acellular bovine pericardium in nude mice

Abstract

Tissue-engineered biologic products may be a viable option in the reconstruction of pelvic organ prolapse (POP). This study was based on the hypothesis that human adipose-derived stem cells (hASCs) are viable in acellular bovine pericardium (ABP), when reseeded by two different techniques, and thus, aid in the reconstruction. To investigate the reseeding of hASCs on ABP grafts by using non-invasive bioluminescence imaging (BLI), and to identify the effective hASCs-scaffold combinations that enabled regeneration. Thirty female athymic nude mice were randomly divided into three groups: In the VIVO group, ABPs were implanted in the subcutaneous pockets and enhanced green fluorescent protein luciferase (eGFPÁLuc)-hASCs (1 Â 10 6 cells/50 mL) were injected on the ABP at the same time. In the VITRO group, the mice were implanted with grafts that ABP were co-cultured with eGFPÁLuc-hASCs in vitro. The BLANK group mice were implanted with ABP only. The eGFPÁLuc-hASCs reseeded on ABP were analyzed by BLI, histology, and immunohistochemistry. The eGFPÁLuc-hASCs reseeded on ABP could be visualized at 12 weeks in vivo. Histology revealed that the VIVO group displayed the highest cell ingrowths, small vessels, and percent of collagen content per unit area. Desmin and a-smooth muscle actin were positive at the same site in the VIVO group cells. However, few smooth muscles were observed in the VITRO and BLANK groups. These results suggest that hASCs reseeded on ABP in vivo during surgery may further enhance the properties of ABP and may promote regeneration at the recipient site, resulting in a promising treatment option for POP.

In vitro evaluation of bovine pericardium after a soft decellularization approach for use in tissue engineering

“…The use of hASCs for tissue repopulation is desirable mainly due to their immunomodulatory and pro‐regenerative properties . In addition, hASCs can be obtained from the patient without an invasive removal procedure, allowing a humanized and autologous transplant.…”

In Vivo Response of Acellular Porcine Pericardial for Tissue Engineered Transcatheter Aortic Valves

“…mentioned that neovascularization contains erythrocytes within the lumen indicating a functional connection with the host vascular system 46 . Many studies have compared MSC-seeded tissue in an in vivo and in vitro environment to determine the effects of seeding on acellular tissue 47,48 . In the current study, the presence of alpha-SMA and vimentin increased after 8 weeks in both the acellular and MSC-seeded porcine pericardium.…”

A novel surgical technique for a rat subcutaneous implantation of a tissue engineered scaffold

“…Key outcomes such as tissue degradation, biomechanical properties, inflammation, and recellularization were analyzed for each method. Evaluating different surgical methods is significant because there is a need to evaluate biocompatibility prior to in vivo implantation of surgical devices (Sarkanen et al, 2012; Wu et al, 2016; Lai et al, 2005; Costa et al, 2005; Tran et al, 2016; Mirsadraee et al, 2007; Wang et al, 2005). However, an inadequate approach to implantation could interfere with the tissue engineered scaffold causing problems with recellularization.…”