<i>In vivo</i> effects of recombinant granulocyte‐colony stimulating factor in athymic nude mice

Pojda, Zygmunt; Aoki, Yoshiro; Tsuboi, Atsushi

doi:10.1038/icb.1990.32

Cited by 2 publications

(1 citation statement)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Neutrophil predominance is the most characteristic cellular feature of the early inflammatory phase of wound healing. 19,20) Granulocyte colony stimulating factor (G-CSF), 21) granulocytemacrophage colony stimulating factor (GM-CSF), 22) macrophage colony stimulating factor (M-CSF), 23) interleukin-1 (IL-1), 24) IL-3 25) and IL-6 26) are known as the cytokines that promote neutrophil production. In the air pouch model by TDM, at day 1 the amount of proinflammation cytokines such as IL-1 and tumor necrosis factor (TNF) in the pouch wall increased.…”

Section: Discussionmentioning

confidence: 99%

Granulomatous Tissue Formation of Shikon and Shikonin by Air Pouch Method.

Sakaguchi¹,

Tsujimura

Ikeda

et al. 2001

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

"The extract of shikon" (SK) and shikonin play important roles in the development of granulomatous tissue formation. To reveal the augmenting effect of SK or shikonin on vascular endothelial growth factor (VEGF) production and neovascularization, we investigated murine granulomatous tissue induced by SK and shikonin, comparing them to pouches in which trehalose 6,6-dimycolate (TDM) was injected. The development of granulomatous tissue formation was evaluated by the wet weight of pouch walls. At day 5 and 7 after SK and shikonin injection, prominent granulomatous tissue formation was detected. Histological observations on the development of granulomatous tissue showed that the pouch was formed in the submuscular connective tissue and necrotic tissue directly facing the cavity and granulomatous tissue developed in the connective tissue. At day 1, VEGF-positive neutrophils accumulated in the pouch wall. Granulomatous tissue formation and neovascularization by injection of SK or shikonin was not more prominent than TDM. However, the present results indicate that SK and shikonin induce neovascularization in granulomatous tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Granulomatous Tissue Formation of Shikon and Shikonin by Air Pouch Method.

Sakaguchi¹,

Tsujimura

Ikeda

et al. 2001

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

Effect of ambient temperature on phenotype and functions of professional phagocytes of athymic nude mice

Větvička¹,

Holub²,

Houštěk³

1993

APMIS

View full text Add to dashboard Cite

Effect of ambient temperature on phenotype and functions of professional phagocytes of athymic nude mice. APMIS 101: 113-1 19, 1993. Cytofluorometric analysis of surface marker expression was performed on myeloid cells isolated from bone marrow, spleen and lymph nodes of nude mice and nu/ + and + / + mice (haired controls) exposed for various time periods to ambient temperature of 22°C or 28°C. A rise in the proportion of cells bearing macrophage markers (MAC-1, MAC-3 and F4/80) in the spleen and of FcR+ cells in all tissues tested was found in 22°C-exposed nudes with high nonshivering thermogenesis. Numbers of MAC-1 + macrophages and actively phagocytic cells increased also in peritoneal exudates. There was a conspicuous predominance of large macrophages in the exudates and the specific markers decreased in density on their surface. Ia expression declined in all tissues tested with the length of exposure to cold. In the granulocytic series (BP-2+ cells), there was a decrease in the bone marrow and lymph nodes and an increase in the spleen and circulation, which suggested an enhanced mobilization and increased production at extramedullary sites in cold-exposed nude mice. The changes in haired mice were negligible.Athymic nude mice appear to be a good model for studying not only thymic dysgenesis and its consequences and compensations, but also the effect of the hairless state (for review see 4). The low insulating capacity of the hairless skin is counteracted by social thermoregulation, endothermic thermogenesis with an increased basal metabolic rate, water and food consumption, and noradrenaline-controlled, thyroxine deiodination-linked nonshivering thermogenesis (6,13). It was established that even a gradient of 6°C in the ambient temperature (between 28" and 22°C) also provokes changes in the immune

show abstract

In vivo effects of recombinant granulocyte‐colony stimulating factor in athymic nude mice

Cited by 2 publications

References 5 publications

Granulomatous Tissue Formation of Shikon and Shikonin by Air Pouch Method.

Granulomatous Tissue Formation of Shikon and Shikonin by Air Pouch Method.

Effect of ambient temperature on phenotype and functions of professional phagocytes of athymic nude mice

Contact Info

Product

Resources

About