<i>In Vivo</i>Efficacy of a Chimeric Peptide Derived from the Conserved Region of the M Protein against Group C and G Streptococci

Nordström, Therése; Malcolm, Jessica; Magor, Graham; Good, Michael F.; Batzloff, Michael R.

doi:10.1128/cvi.00140-12

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…Although published data on preclinical efficacy of vaccine candidates in SDSE is limited beyond preliminary data on the J8 peptide 47 , the prevalence of conserved antigens suggest there may be cross-species effects of vaccines intended to target S. pyogenes . However, the sequence-based approach used in this study does not consider potential conserved structural epitopes which may confer antibody cross-reactivity or cross-opsonisation to divergent alleles.…”

Section: Discussionmentioning

confidence: 99%

Inter-species gene flow drives ongoing evolution of Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis

Xie,

Morris,

Hayes

et al. 2024

Nat Commun

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Streptococcus dysgalactiae subsp. equisimilis (SDSE) is an emerging cause of human infection with invasive disease incidence and clinical manifestations comparable to the closely related species, Streptococcus pyogenes. Through systematic genomic analyses of 501 disseminated SDSE strains, we demonstrate extensive overlap between the genomes of SDSE and S. pyogenes. More than 75% of core genes are shared between the two species with one third demonstrating evidence of cross-species recombination. Twenty-five percent of mobile genetic element (MGE) clusters and 16 of 55 SDSE MGE insertion regions were shared across species. Assessing potential cross-protection from leading S. pyogenes vaccine candidates on SDSE, 12/34 preclinical vaccine antigen genes were shown to be present in >99% of isolates of both species. Relevant to possible vaccine evasion, six vaccine candidate genes demonstrated evidence of inter-species recombination. These findings demonstrate previously unappreciated levels of genomic overlap between these closely related pathogens with implications for streptococcal pathobiology, disease surveillance and prevention.

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Section: Discussionmentioning

confidence: 99%

Inter-species gene flow drives ongoing evolution of Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis

Xie,

Morris,

Hayes

et al. 2024

Nat Commun

Self Cite

View full text Add to dashboard Cite

show abstract

“…Over 25% of MGE insertion regions and MGE clusters were found to be conserved across Although published data on preclinical efficacy of vaccine candidates in SDSE is limited beyond preliminary data on the J8 peptide 46 , the prevalence of conserved antigens suggest there may be cross-species effects of vaccines intended to target S. pyogenes. However, the sequence-based approach used in this study does not consider potential conserved structural epitopes which may confer antibody cross-reactivity or cross-opsonisation to divergent alleles.…”

Section: Discussionmentioning

confidence: 99%

Inter-species gene flow drives ongoing evolution ofStreptococcus pyogenesandStreptococcus dysgalactiaesubsp.equisimilis

Xie

Morris

Hayes

et al. 2023

Preprint

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Streptococcus dysgalactiae subsp. equisimilis (SDSE) is an emerging cause of human infection with invasive disease incidence and clinical manifestations comparable to the closely related species, Streptococcus pyogenes. Through systematic genomic analyses of 501 disseminated SDSE strains, we demonstrate extensive overlap between the genomes of SDSE and S. pyogenes. More than 75% of core genes are shared between the two species with one third demonstrating evidence of cross-species recombination. Twenty-five percent of mobile genetic element (MGE) clusters and 16 of 55 SDSE MGE insertion regions were found across species. Assessing potential cross-protection from leading S. pyogenes vaccine candidates on SDSE, 12/34 preclinical vaccine antigen genes were shown to be present in >99% of isolates of both species. Relevant to possible vaccine evasion, six vaccine candidate genes demonstrated evidence of inter-species recombination. These findings demonstrate previously unappreciated levels of genomic overlap between these closely related pathogens with implications for streptococcal pathobiology, disease surveillance and prevention.

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Serological Responses to TargetStreptococcus pyogenesVaccine Antigens in Patients With Proven Invasive β-Hemolytic Streptococcal Infections

Langworthy,

Taggart,

Smith

et al. 2024

The Journal of Infectious Diseases

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Background Rising incidence of invasive beta-haemolytic streptococcal (iBHS) infections has prompted consideration of vaccination as a preventative strategy for at-risk populations. The benefits of a vaccine targeting Lancefield group A (Streptococcus pyogenes; Strep A) would increase if cross-species immunity against Lancefield groups C/G (Streptococcus dysgalactiae subspecies equisimilis; SDSE) and B (Streptococcus agalactiae; GBS) was demonstrated. Methods A prospective, observational study of adult patients with iBHS infections due to Strep A, SDSE or GBS. Antibody responses to six Strep A candidate antigens were assayed on acute and convalescent sera. A serological response was defined as an increase of >0.2log10 arbitrary units/mL (AU/mL). Results Sixty-seven participants were enrolled. Thirty-three participants were included in the final analysis (12, 11 and 10 with Strep A, SDSE and GBS, respectively). The median serological response for participants with Strep A was significant for all tested antigens (median >0.2log10 difference between acute and convalescent samples; P<0.05 for all). Those with SDSE had comparable and significant median (IQR) responses to streptolysin-O (0.65 [0.36-1.67], P=0.004), S. pyogenes adhesion and division protein (0.68 [0.36-1.63], P=0.005) and C5a peptidase (ScpA; 0.30 [0.23-1.06]), P=0.004). GBS responses were limited to ScpA only (0.34 [0.08-0.52], P=0.05). Conclusion Patients with invasive Strep A infection mount robust antibody responses to six non-M protein vaccine candidate antigens. Similar significant responses to C5a peptidase in those with invasive SDSE and GBS infection highlight the importance of further research into cross-species protection and immunological correlates of vaccine efficacy.

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In VivoEfficacy of a Chimeric Peptide Derived from the Conserved Region of the M Protein against Group C and G Streptococci

Cited by 4 publications

References 32 publications

Inter-species gene flow drives ongoing evolution of Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis

Inter-species gene flow drives ongoing evolution of Streptococcus pyogenes and Streptococcus dysgalactiae subsp. equisimilis

Inter-species gene flow drives ongoing evolution ofStreptococcus pyogenesandStreptococcus dysgalactiaesubsp.equisimilis

Serological Responses to TargetStreptococcus pyogenesVaccine Antigens in Patients With Proven Invasive β-Hemolytic Streptococcal Infections

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