In vivo feasibility test using transparent carbon nanotube‐coated polydimethylsiloxane sheet at brain tissue and sciatic nerve

Abstract

Carbon nanotubes, with their unique and outstanding properties, such as strong mechanical strength and high electrical conductivity, have become very popular for the repair of tissues, particularly for those requiring electrical stimuli. Polydimethylsiloxane (PDMS)-based elastomers have been used in a wide range of biomedical applications because of their optical transparency, physiological inertness, blood compatibility, non-toxicity, and gas permeability. In present study, most of artificial nerve guidance conduits (ANGCs) are not transparent. It is hard to confirm the position of two stumps of damaged nerve during nerve surgery and the conduits must be cut open again to observe regenerative nerves after surgery. Thus, a novel preparation method was utilized to produce a transparent sheet using PDMS and multiwalled carbon nanotubes (MWNTs) via printing transfer method. Characterization of the PDMS/MWNT (PM) sheets revealed their unique physicochemical properties, such as superior mechanical strength, a certain degree of electrical conductivity, and high transparency. Characterization of the in vitro and in vivo usability was evaluated. PM sheets showed high biocompatibility and adhesive ability. In vivo feasibility tests of rat brain tissue and sciatic nerve revealed the high transparency of PM sheets, suggesting that it can be used in the further development of ANGCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1736-1745, 2017.

A printing-spray-transfer process for attaching biocompatible and antibacterial coatings to the surfaces of patient-specific silicone stents

“…This has been confirmed in previous studies of different cell types, including bone, embryonic, myocardial, and neuronal cells and skin fibroblasts [24,59,60]. Thus, because of the lack of attachment sites on the pristine silicone surface, most cells were washed away during the rinsing steps of the immunofluorescence staining [60]. Further comparing the cell morphologies between the spray-deposition and printing-spray-transfer coatings showed that the HBECs on the latter were more intensely aggregated, with larger surface coverage and a more complex intercellular network compared with those on the former.…”

“…These results seemed to conflict with those of the CCK-8, likely due to the MWCNT network of the AgNPs/CNT coatings on the silicone substrate, which significantly positively affected the HBEC attachment. This has been confirmed in previous studies of different cell types, including bone, embryonic, myocardial, and neuronal cells and skin fibroblasts [24,59,60]. Thus, because of the lack of attachment sites on the pristine silicone surface, most cells were washed away during the rinsing steps of the immunofluorescence staining [60].…”

“…Spray deposition and transfer printing techniques are widely applied in flexible electronics [38,[54][55][56][57] and biomedical devices [58][59][60] for their flexibility and convenience compared with existing techniques (dip coating, hot pressing, vacuum filtration and electrostatic spray deposition). However, physicochemical methods are needed to address the poor adhesion problems of the coatings deposited via spray deposition.…”

Nanoparticle‐based nanocomposite coatings with postprocessing for enhanced antimicrobial capacity of polymeric film

“…Therefore, we focused on making antimicrobial coating sheets as transparent as possible. To test the transparency of samples, images were taken by placing samples on the university logo sheets to increase the clarity at a fixed height and under stable light conditions (C. Wang et al, 2017).…”

Electrical welding technology in restoring the integrity of the injured peripheral nerve: review of literature and own experimental research

“…Vol. 26, N2, 2020 [19,20,[28][29][30][31][32][33][34][35][36], нанокомпозитного [37] чи найменш вивченого -електрозварного [38].…”