In vivo grafting of wild Lens species to Vicia faba rootstocks

Abstract

Faba bean (Vicia faba L.) was used as the rootstock for lentil scions to test the feasibility of using in vivo inter-generic grafting techniques as a substitute for root induction and as a tool in lentil genetic improvement. An accession of each of the six wild Lens species was used as the scion in grafts to faba bean breeding line FB50-9 rootstock. Successful grafts were obtained for all species with survival of grafts to seed maturity between 70.7 and 87.7% except for Lens orientalis PI 72735 with 55.3% survival. Days to flower remained the same after grafting, except for scions of L. nigricans PI 72560 and L. orientalis PI 72735 which had a lag phase of 9 and 7 d, respectively. For all six wild species, seed diameter and seed weight were not significantly different between non-grafted controls and scions grafted onto faba bean rootstocks. This simple approach opens the possibility of using in vivo grafting techniques to rescue inter-specific hybrids of lentil. The technique has potential as a useful tool in lentil breeding, as a means of improving seed multiplication rate of rare genetic resources of wild lentil and as a way to reduce the costs of germplasm multiplication of wild lentil species.

Effect of Root Trainer on the Success of Grafting and the Survival Rate of Cashew Tree [Anacardium occidentale L.(Anacardiaceae)] in Field

“…Grafting is one of the techniques used for propagation in many plants, including cashew trees. Its success depends on several factors including the rootstock substrate, the age of the rootstock, the length of the graft, the diameter of the graft and the rootstock, the presence of leaves on the rootstock and the appearance of the graft [19,20] but also the grafting season and the genotype [21,22].…”

Screening of wild and cultivated lentil germplasm for resistance to stemphylium blight

“…Research is underway at the CDC to develop lentil cultivars with genetic resistance to SB as part of a long-term strategy for reducing potential economic losses caused by the disease. Special techniques such as embryo rescue and grafting required to obtain fertile hybrids from distant or interspecies crosses (Fiala et al , 2009), tissue culture for multiplication of F 1 plants from rescued embryo (Shyamoli Saha, CDC, University of Saskatchewan, Canada, unpublished results), grafting to faba bean (Yuan et al , 2011) and reproduction using cutting for multiplication to achieve rapid disease screening (Vail et al , 2012) have been successfully established and have made it feasible to reliably transfer resistance from wild to cultivated lentil. The results from the present study contribute to scientific knowledge that will help with the development of an effective breeding strategy for SB resistance in lentil.…”

Breeding Annual Grain Legumes for Sustainable Agriculture: New Methods to Approach Complex Traits and Target New Cultivar Ideotypes

“…Gulati et al (2001) reported a success rate of 84-96% with a micro-grafting of in vitro regenerated shoots from cotyledonary nodes compared to a much lower efficiency obtained with in vitro root induction. The grafting technique has been adapted for many applications (Yuan et al, 2011;Gurusamy et al, 2012) in rescuing genotypes, physiological studies and gene function studies.…”