In VivoMagnetic Resonance Imaging of Type I Collagen Scaffold in Rat: Improving Visualization of Bladder and Subcutaneous Implants

Abstract

Noninvasive monitoring of implanted scaffolds is important to understand their behavior and role in tissue engineering, in particular to follow their degradation and interaction with host tissue. Magnetic resonance imaging (MRI) is well suited for this goal, but its application is often hampered by the low contrast of scaffolds that are prepared from biomaterials such as type I collagen. The aim of this study was to test iron oxide particles incorporation in improving their MRI contrasts, and to follow their degradation and tissue interactions. Scaffolds with and without iron oxide particles were implanted either subcutaneously or on the bladder of rats. At predetermined time points, in vivo MRI were obtained and tissues were then harvested for histology analysis and transmission electron microscopy. The result showed that the incorporation of iron oxide particles improved MRI contrast of the implants, providing information on their location, shapes, and degradation. Second, the host tissue reaction to the type I collagen implants could be observed in both MRI and histology. Finally, MRI also revealed that the degradation and host tissue reaction of iron particles-loaded scaffolds differed between subcutaneous and bladder implantation, which was substantiated by histology.

Enhanced bone regeneration and visual monitoring via superparamagnetic iron oxide nanoparticle scaffold in rats

“…The relative mean greyscale value was determined using the formula: Relative mean greyscale value = (Greyscale value of the left side)/(Greyscale value of the right side) (Y. Sun, Geutjes, et al, ).…”

“…Recently, Y. Sun, Geutjes, Oosterwijk, and Heerschap () described non‐invasive monitoring of cellular behaviour around gelatin scaffolds and their distribution in the bladder and subcutaneous tissue of mice.…”

Developing quantitative MRI parameters to characterize host response and tissue ingrowth into collagen scaffolds

“…Previous studies have reported changes in T 2 and/or ADC values to be associated with other mechanisms like ECM architecture remodeling, [24][25][26][27] fibrous tissue formation, [28][29][30] tissue hydration, 26,[31][32][33] and inflammation, [34][35][36] which may occur concurrently with cellular infiltration and hence affect the T 2 and ADC values. Moreover, the relationship between total cellularity, T 2 , and ADC values has also been well documented in the cancer literature.…”

Effectiveness of Eccentric Exercise for the Management of Rotator Cuff Tendinopathy: A Critical Review

“…However, although the study examined plasma concentrations of PICP and ICTP, the changes of collagen type I formation could not directly be observed. Previous studies suggested that MRI or ultrasound can observe the changes in collagen type I formation [29,30]. More medical examinations should be included in further studies.…”