<i>In Vivo</i> Perturbation of Membrane-Associated Calcium by Freeze-Thaw Stress in Onion Bulb Cells

Arora, Rajeev; Palta, Jiwan P.

doi:10.1104/pp.87.3.622

Cited by 55 publications

(15 citation statements)

References 26 publications

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“…Evidence in support of this comes from our observations on a specific loss of membrane-associated Ca2" following a reversible and irreversible freeze-thaw-induced injury (1). A dramatic loss in K+ uptake and plasma membrane ATPase activity has been shown to parallel the loss of membraneassociated calcium in cucumber roots (13,28).…”

Section: Injurymentioning

confidence: 65%

“…Onion bulbs were slowly frozen (cooling rate of 1-2°C/h) to -5.0°C according to the procedure of Arora and Palta (1). Following a complete thaw the onion was divided into two halves.…”

Section: Freeze-thaw Treatment and Recoverymentioning

confidence: 99%

“…The pieces were then quickly rinsed with distilled deionized H20 and transferred to 20 mL of fresh distilled deionized H20 and the above mentioned steps were repeated twice and conductivity of the leachate was recorded for the second (C2) and the third (C3) h. The conductivity was finally recorded (Ck) for the same tissue after it was heat killed in fresh 20 mL distilled deionized water. All four conductivity readings were added (C, + C2 + C3 + CA) to give total ions present (1) in the tissue at the beginning. From this, the amount of ions present in the tissue at the end of the first hour of shaking (T,) was calculated from the expression T, = T -C,.…”

Section: Ion Efflux Ratementioning

confidence: 99%

“…For this purpose, onion scale tissue was used because the visual symptoms of injury and recovery can be followed very clearly (1,(16)(17)(18)(19). Using vanadate as a specific inhibitor of plasma membrane ATPase (5, 11), we show that recovery of ATPase functions coincides with the recovery of the injured tissue.…”

mentioning

confidence: 97%

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A Loss in the Plasma Membrane ATPase Activity and Its Recovery Coincides with Incipient Freeze-Thaw Injury and Postthaw Recovery in Onion Bulb Scale Tissue

Arora¹,

Palta²

1991

Plant Physiol.

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Plasma membrane ATPase has been proposed to be functionally altered during early stages of injury caused by a freeze-thaw stress. Complete recovery from freezing injury in onion cells during the postthaw period provided evidence in support of this proposal. During recovery, a simultaneous decrease in ion leakage and disappearance of water soaking (symptoms of freezethaw injury) has been noted. Since reabsorption of ions during recovery must be an active process, recovery of plasma membrane ATPase (active transport system) functions has been implicated. In the present study, onion (Allium cepa L. cv Downing Yellow Globe) bulbs were subjected to a freeze-thaw stress which resulted in a reversible (recoverable) injury. Plasma membrane ATPase activity in the microsomes (isolated from the bulb scales) and ion leakage rate (efflux/hour) from the same scale tissue were measured immediately following thawing and after complete recovery. In injured tissue (30-40% water soaking), plasma membrane ATPase activity was reduced by about 30% and this was paralleled by about 25% higher ion leakage rate. As water soaking disappeared during recovery, the plasma membrane ATPase activity and the ion leakage rate returned to about the same level as the respective controls. Treatment of freeze-thaw injured tissue with vanadate, a specific inhibitor of plasma membrane ATPase, during postthaw prevented the recovery process. These results indicate that recovery of freeze-injured tissue depends on the functional activity of plasma membrane ATPase.In nature, herbaceous plants experience slow cooling rates (1-2°C/h) during freezing event (9,24). This results in extracellular freezing when ice first forms in the dilute apoplastic solution. Such freezing, depending on the degree and duration of low temperature and subsequent thawing, result in increased efflux of ions and organic solutes. The tissue also takes on a water-soaked appearance. The major cation that leaks out ofthe cell is known to be K+ (17, 22). This enhanced efflux of ions results from a higher efflux than the influx rates.

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Section: Injurymentioning

confidence: 65%

“…Onion bulbs were slowly frozen (cooling rate of 1-2°C/h) to -5.0°C according to the procedure of Arora and Palta (1). Following a complete thaw the onion was divided into two halves.…”

Section: Freeze-thaw Treatment and Recoverymentioning

confidence: 99%

Section: Ion Efflux Ratementioning

confidence: 99%

mentioning

confidence: 97%

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A Loss in the Plasma Membrane ATPase Activity and Its Recovery Coincides with Incipient Freeze-Thaw Injury and Postthaw Recovery in Onion Bulb Scale Tissue

Arora¹,

Palta²

1991

Plant Physiol.

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“…Most of these studies measured total cellular fluorescence, which reflects autofluorescence and CTC fluorescence from numerous organelles as well as CTC fluorescence from the plasma membrane-cell wall complex (eg., Cramer et al 1985;Renard-Rooney et al 1993). Tirlapur and Cresti (1992) used video image analysis in conjunction with CTC fluorescence to map the spatial distribution of mCa in growing tobacco pollen tubes, and Arora and Palta (1988) used similar procedures to examine the influence of supplemental Ca treatments on mCa of onion epidermal cells. Despite considerable use of CTC as a fluorescent probe for visualization of mCa, we are unaware of any studies examining mCa in leaf tissues of woody plants, or procedural descriptions for estimating mCa.…”

Section: Introductionmentioning

confidence: 99%

Relative quantification of membrane-associated calcium in red spruce mesophyll cells

Borer

DeHayes

Schaberg

et al. 1997

Trees

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AbstractmWe describe a method for localizing and comparing relative amounts of plasma membrane-associated calcium ions (mCa) in complex tissues and verify the procedure for mesophyll cells of red spruce (Picea rubens Sarg.) needles. This technique incorporates epifluorescence microscopy using the fluorescent probe chlorotetracycline (CTC) with computer image processing and analysis. Using an appropriate standardization for image brightness, the procedure allows relative quantitative comparison of CTCfluorescence in the plasma membrane-cell wall region that corresponds to relative amounts of mCa. The technique effectively discerned mCa differences among red spruce needle sections exposed to treatments designed to alter mCa levels in vitro. Estimates of mCa for nine red spruce seedlings, were highly repeatable over a 6 week period in late summer. This repeatability verifies that the described methods produce reliable and reproducible estimates of foliar mCa in woody plant foliage. By incorporating image analysis, this technique allows for relative quantitative estimates of mCa specific to the physiologicallyimportant and labile pool of Ca associated with the plasma membrane-wall complex. Such measurements have not previously been reported for woody plant tissues and thus may provide new insights into the relative roles and responsiveness of mCa vs total foliar Ca pools.

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Plant Antifreeze Proteins

Wisniewski

Willick

Duman

et al. 2020

Antifreeze Proteins Volume 1

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In Vivo Perturbation of Membrane-Associated Calcium by Freeze-Thaw Stress in Onion Bulb Cells

Cited by 55 publications

References 26 publications

A Loss in the Plasma Membrane ATPase Activity and Its Recovery Coincides with Incipient Freeze-Thaw Injury and Postthaw Recovery in Onion Bulb Scale Tissue

A Loss in the Plasma Membrane ATPase Activity and Its Recovery Coincides with Incipient Freeze-Thaw Injury and Postthaw Recovery in Onion Bulb Scale Tissue

Relative quantification of membrane-associated calcium in red spruce mesophyll cells

Plant Antifreeze Proteins

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