<i>In vivo</i>prediction of the nutrient status of individual microalgal cells using Raman microspectroscopy

Heraud, Philip; Beardall, John; McNaughton, Donald; Wood, Bayden Robert

doi:10.1111/j.1574-6968.2007.00861.x

Cited by 91 publications

(91 citation statements)

References 33 publications

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“…However, few studies have been undertaken of carotenoids in vivo [7][8][9]. Classical techniques of pigment identification and discrimination are absorption spectroscopy and high-performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Jehlička

Edwards

Osterrothová

et al. 2014

Phil. Trans. R. Soc. A.

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In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles, in contrast with others which can be considered as biomarkers of other genera. The limits of application of Raman spectroscopy are discussed for a few cases where the current Raman spectroscopic approach does not allow discriminating structurally very similar carotenoids. The database reported can be used for applications in geobiology and exobiology for the detection of pigment signals in natural settings.

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Section: Introductionmentioning

confidence: 99%

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Jehlička

Edwards

Osterrothová

et al. 2014

Phil. Trans. R. Soc. A.

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“…Thus, the presence of ␤-carotene and chlorophyll a was demonstrated in vivo in individual cells within colonies of Dunaliella tertiolecta using confocal Raman microscopy with excitation at 532 nm (96). Confocal Raman spectroscopy was used to differentiate and map the relation between a carotene-containing yeast and Pseudomonas aeruginosa cells in living, hydrated, composite biofilms on the basis of their spectral signatures (97).…”

Section: Advanced Raman Spectroscopic Techniques In Microbiologymentioning

confidence: 99%

Raman Spectroscopy of Microbial Pigments

Jehlička

Edwards

Oren

2014

Appl Environ Microbiol

151

114

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Raman spectroscopy is a rapid nondestructive technique providing spectroscopic and structural information on both organic and inorganic molecular compounds. Extensive applications for the method in the characterization of pigments have been found. Due to the high sensitivity of Raman spectroscopy for the detection of chlorophylls, carotenoids, scytonemin, and a range of other pigments found in the microbial world, it is an excellent technique to monitor the presence of such pigments, both in pure cultures and in environmental samples. Miniaturized portable handheld instruments are available; these instruments can be used to detect pigments in microbiological samples of different types and origins under field conditions.

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“…Spectroscopic approaches offer substantial benefits over traditional methods, being inexpensive, quantitative, objective, rapid, high throughput, sensitive and inherently multivariate (Hirschmugl et al, 2005;Heraud et al, 2005Heraud et al, , 2007aHeraud et al, , 2008Jebsen et al, 2012;Sackett et al, 2013). The use of spectroscopic techniques such as Raman and Fourier transform infrared (FTIR) spectroscopy in phytoplankton studies has been widely explored (Heraud et al, 2007b;Heraud et al, 2008;Domenighini and Giordano, 2009;Dean et al, 2012Dean et al, , 2010.…”

Section: Introductionmentioning

confidence: 99%

Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy

et al. 2015

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Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Southern Ocean food web. Prevailing environmental conditions influence diatom phenotypic traits (for example, photophysiology, macromolecular composition and morphology), which in turn affect the transfer of energy, carbon and nutrients to grazers and higher trophic levels, as well as oceanic biogeochemical cycles. The paucity of phenotypic data on Southern Ocean phytoplankton limits our understanding of the ecosystem and how it may respond to future environmental change. Here we used a novel approach to create a 'snapshot' of cell phenotype. Using mass spectrometry, we measured nitrogen (a proxy for protein), total carbon and carbon-13 enrichment (carbon productivity), then used this data to build spectroscopy-based predictive models. The models were used to provide phenotypic data for samples from a third sample set. Importantly, this approach enabled the first ever rate determination of carbon productivity from a single time point, circumventing the need for timeseries measurements. This study showed that Chaetoceros simplex was less productive and had lower protein and carbon content during short-term periods of high salinity. Applying this new phenomics approach to natural phytoplankton samples could provide valuable insight into understanding phytoplankton productivity and function in the marine system.

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In vivoprediction of the nutrient status of individual microalgal cells using Raman microspectroscopy

Cited by 91 publications

References 33 publications

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology

Raman Spectroscopy of Microbial Pigments

Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy

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