<i>In Vivo</i>Studies on Testosterone Metabolism by Skin of Normal Males and Patients with the Syndrome of Testicular Feminization

Mauvais-Jarvis, P; Bercovici, J. P.; Gauthier, Francis

doi:10.1210/jcem-29-3-417

Cited by 58 publications

(17 citation statements)

References 8 publications

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“…Previous studies on androgen resistance syndromes have revealed the importance of the androgen receptor for androgen-dependent hair growth (4)(5)(6). More recently, an increased androgen binding capacity was observed in scalp hair cells of balding men (7).…”

Section: Introductionmentioning

confidence: 97%

The 5alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals

Oliveira

Lhullier

Brum

et al. 2003

Braz J Med Biol Res

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The aim of the present study was to determine the expression of the genes for type 1 (SDR5A1) and type 2 (SDR5A2) 5α-reductase isoenzymes in scalp hairs plucked from 33 hirsute patients (20 with polycystic ovary syndrome and 13 with idiopathic hirsutism) and compare it with that of 10 men and 15 normal women. SDR5A1 and SDR5A2 expression was estimated by RT-PCR using the gene of the ubiquitously expressed protein ß 2 -microglobulin as an internal control. The results are expressed as arbitrary units in relation to ß 2 -microglobulin absorbance (mean ± SEM). SDR5A2 expression was not detected in any hair samples analyzed in this study. No differences were found in SDR5A1 mRNA levels between men and normal women (0.78 ± 0.05 vs 0.74 ± 0.06, respectively). SDR5A1 gene expression in the cells of hair plucked from the scalp of normal women (0.85 ± 0.04) and of women with polycystic ovary syndrome (0.78 ± 0.05) and idiopathic hirsutism (0.80 ± 0.06) was also similar. These results indicate that SDR5A1 gene expression in the follicular keratinocytes from the vertex area of the scalp seems not to be related to the differences in hair growth observed between normal men and women and hirsute patients. Further studies are needed to investigate the expression of the 5α-reductase genes in other scalp follicular compartments such as dermal papillae, and also in hair follicles from other body sites, in order to elucidate the mechanism of androgen action on the hair growth process and related diseases.

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Section: Introductionmentioning

confidence: 97%

The 5alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals

Oliveira

Lhullier

Brum

et al. 2003

Braz J Med Biol Res

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“…This level was significantly higher (P < 0.05) in patients with feminizing testes than in normal males. The level increased dramatically after administration of a low dose of estrogen whereas this effect was not observed in normal males under the same experimental conditions.In light of these results the defect of extrahepatic 5a-reduction of testosterone observed in patients with Part of this work has been published as a preliminary communication (14). …”

mentioning

confidence: 92%

“…In light of these results the defect of extrahepatic 5a-reduction of testosterone observed in patients with Part of this work has been published as a preliminary communication (14).…”

mentioning

confidence: 92%

“…More recently, it has been reported that 17,8-hydroxy-5a-androstan-3-one (androstanolone) is the only metabolite present in the prostatic nuclei after intravenous administration of radiocative testosterone to rats or after incubation of rat prostate with the same precursor (12,13 (14). The urine was collected over 3 days after the administration of tracers.…”

Section: Introductionmentioning

confidence: 99%

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Studies on testosterone metabolism in subjects with testicular feminization syndrome

Mauvais-Jarvis¹,

Bercovici²,

Crépy³

et al. 1970

J. Clin. Invest.

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115

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A B S T R A C T The metabolism of radioactive testosterone simultaneously administered intravenously and either orally or percutaneously has been studied in seven patients with the syndrome of testicular feminization and compared with that of normal males and females. This investigation was carried out in order to determine the relative contribution to urinary 17-oxo and 17p-hydroxy androstane steroids of labeled testosterone, according to its mode of administration. In normal males the yields of urinary 5a-androstane-3a,17,-diol (androstanediol) originating from either an intravenous or a percutaneous dose of testosterone were respectively 3 and 6 times higher than those arising from an oral dose which perfuses the liver directly. These data indicate that in normal males, testosterone might be 5a-hydrogenated outside the liver. By contrast in patient with feminizing testes, because the contribution to androstanediol of radioactive testosterone is identical whatever its mode of administration, the extrahepatic 5a-reduction of this substrate seems very unlikely.The metabolic abnormalities observed in patients with testicular feminization syndrome may be reproduced in normal males by estrogen treatment. Nevertheless, the sensitivity of the patients to estrogen seems to be 10 times greater than that of normal males. This sensitivity was appreciated from the reduction of radioactive testosterone intravenously injected to urinary 17P-hydroxy-5a-androstan-3-one and androstanediol and also from the level of plasma binding for testosterone. This level was significantly higher (P < 0.05) in patients with feminizing testes than in normal males. The level increased dramatically after administration of a low dose of estrogen whereas this effect was not observed in normal males under the same experimental conditions.In light of these results the defect of extrahepatic 5a-reduction of testosterone observed in patients with Part of this work has been published as a preliminary communication (14).

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“…In the abnormality of sexual development that goes by the misnomer of testicular feminization, indirect evidence has been presented suggesting that the defect, which has been known to be present in target cells, involves the enzyme that converts testosterone to its active intracellular form, dihydro testosterone (261,296). Other interpretations of the evidence are possible (262).…”

mentioning

confidence: 99%

Human Genetics

McKusick

1970

Annu. Rev. Genet.

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In VivoStudies on Testosterone Metabolism by Skin of Normal Males and Patients with the Syndrome of Testicular Feminization

Cited by 58 publications

References 8 publications

The 5alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals

The 5alpha-reductase type 1, but not type 2, gene is expressed in anagen hairs plucked from the vertex area of the scalp of hirsute women and normal individuals

Studies on testosterone metabolism in subjects with testicular feminization syndrome

Human Genetics

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