<i>In vivo</i> study of the physiological impact of stratum corneum sampling methods

Rosado, Catarina; Rodrigues, Luís Monteiro

doi:10.1046/j.1467-2494.2003.00171.x

Cited by 14 publications

(15 citation statements)

References 20 publications

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“…After SC stripping, the TEWL increases but rapidly returns to normal within a couple of days [23,24,28] . This is probably due to the extrusion of preformed keratinosomes which represent a reserve to control the effects of an acute threat.…”

Section: Discussionmentioning

confidence: 99%

“…The assessment procedure started at D15. In each subject, the same number of cyanoacrylate skin surface strippings (CSSS) was harvested in order to compromise the SC barrier function [22][23][24]28] and to obtain preconditioned baseline TEWL values above 15 g/m 2 /h on each of the 3 test sites. The deepest collected CSSS was examined by scanning electron microscopy.…”

Section: Methodsmentioning

confidence: 99%

“…In an experimental setting, tape stripping provides a model of controlled SC damage and repair [16][17][18][19][20][21][22][23][24] . Using this method, some studies have documented the infl uence of topicals on its repair [22,[25][26][27][28] .…”

Section: Introductionmentioning

confidence: 99%

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Repair Kinetics of the Stratum corneum under Repeated Insults

Uhoda

Franchimont

Debatisse³

et al. 2004

Exog Dermatol

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Background: The stratum corneum (SC) structure and functions are altered by surfactants. Enhancing the repair mechanisms is a goal for some skin care formulations. Aim: To design an experimental procedure allowing the discrimination of repair efficacy for topical products applied to threatened SC. Method: Controlled SC strippings followed by repeated forearm soak sessions in surfactant solutions were used to compromise the SC barrier function in 20 volunteers. Two test formulations were compared. They were applied twice daily for 2 weeks before initiating the SC damages, and for the next 2 weeks while sustaining the procedure of soak sessions. Daily assessments involved objective measurements of parameters including transepidermal water loss, the value of the passive sustainable SC hydration and the epidermal turnover rate using the dihydroxyacetone test. Result: The experimental procedure allowed to clearly distinguish the differences in the kinetics of SC repair between the topical formulations. In the present study design, the repair of the barrier function mirrored the SC renewal. Conclusion: The present experimental design slows down the repair rate of the SC barrier function. Thus, it increases the sensitivity of biometrological measurements assessing some of the major SC properties. It also better mimics the common in vivo situation experienced by subjects chronically exposed to irritant xenobiotics.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Repair Kinetics of the Stratum corneum under Repeated Insults

Uhoda

Franchimont

Debatisse³

et al. 2004

Exog Dermatol

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“…Thus, measuring the reflectance of light in the SWIR range was proposed for interrogation of moisture and microscopic structural changes . We further surmise that the SWIR light reflectance sensitivity to tissue factors and moisture could also make it an important marker of skin tissue viability in burns, as suggested by the correlation between water loss and epidermal barrier dysfunction . Previous literature, especially in dermatologic journals, has characterized the composition of healthy cutaneous tissues.…”

Section: Introductionmentioning

confidence: 99%

“…Namely, strong water signals have been characterized within the stratum corneum and viable epidermis . Furthermore, studies investigating compositional changes after mechanical damage to the cutaneous layer have demonstrated increases in transepidermal water loss, that is, decrease in moisture content . Thus, in Figure , we illustrate the derived postulate that in viable skin that is well hydrated, increased water content preferentially attenuates the reflectance of the 1,940 nm wavelength compared to the 1,650 or 1,200 nm.…”

Section: Introductionmentioning

confidence: 99%

Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns

Mironov

Hwang

Nemzek

et al. 2019

Wound Repair Regeneration

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Existing clinical approaches and tools to measure burn tissue destruction are limited resulting in misdiagnosis of injury depth in over 40% of cases. Thus, our objective in this study was to characterize the ability of short‐wave infrared (SWIR) imaging to detect moisture levels as a surrogate for tissue viability with resolution to differentiate between burns of various depths. To accomplish our aim, we constructed an imaging system consisting of a broad‐band Tungsten light source; 1,200‐, 1,650‐, 1,940‐, and 2,250‐nm wavelength filters; and a specialized SWIR camera. We initially used agar slabs to provide a baseline spectrum for SWIR light imaging and demonstrated the differential absorbance at the multiple wavelengths, with 1,940 nm being the highest absorbed wavelength. These spectral bands were then demonstrated to detect levels of moisture in inorganic and in vivo mice models. The multiwavelength SWIR imaging approach was used to diagnose depth of burns using an in vivo porcine burn model. Healthy and injured skin regions were imaged 72 hours after short (20 seconds) and long (60 seconds) burn application, and biopsies were extracted from those regions for histologic analysis. Burn depth analysis based on collagen coagulation histology confirmed the formation of superficial and deep burns. SWIR multispectral reflectance imaging showed enhanced intensity levels in long burned regions, which correlated with histology and distinguished between superficial and deep burns. This SWIR imaging method represents a novel, real‐time method to objectively distinguishing superficial from deep burns.

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Human Epidermal Barrier May Be Quantitatively Described by Compartmental Analysis of Water Dynamics

Rodrigues

Rosado

2015

Agache’s Measuring the Skin

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In vivo study of the physiological impact of stratum corneum sampling methods

Cited by 14 publications

References 20 publications

Repair Kinetics of the Stratum corneum under Repeated Insults

Repair Kinetics of the Stratum corneum under Repeated Insults

Short‐wave infrared light imaging measures tissue moisture and distinguishes superficial from deep burns

Human Epidermal Barrier May Be Quantitatively Described by Compartmental Analysis of Water Dynamics

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