In Vivo Study of the Efficacy of the Aromatic Water of Zataria multiflora on Hydatid Cysts

Abstract

c Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) were employed to determine the chemical composition of the essential oil (EO) from aromatic water (AW) of Zataria multiflora. Thymol (66.9%), carvacrol (15.2%), and carvone (7.3%) were found to be the major EO constituents. Eighty laboratory BALB/c mice were infected intraperitoneally by injection of 1,500 viable protoscolices and were divided into prevention (40 mice) and therapeutic (40 mice) groups. To prove the preventive effect of the Z. multiflora AW on development of hydatid cysts, the 40 infected mice were allocated into three treatment groups, including the albendazole group (10 mice that received 150 mg/kg body weight/day for 10 days), the Z. multiflora AW group (15 mice that received 20 ml/liter in drinking water for 8 months), and a control group (15 mice that received no treatment). To estimate the therapeutic effect of the Z. multiflora AW on the hydatid cyst, after 8 months of infection, the 15 remaining mice were allocated into three experimental treatment groups of five animals each, including the albendazole group (300 mg/ kg/day for 20 days), Z. multiflora AW group (40 ml/liter in drinking water for 30 days), and control group (no treatment). All mice were then euthanized, and the sizes and weights of the cysts as well as their ultrastructural changes were investigated. The weights and sizes of the hydatid cysts significantly decreased upon treatment with the Z. multiflora AW in both the preventive and therapeutic groups (P < 0.05). The results of scanning electron microscopy also showed considerable damage in the germinal layer of the hydatid cysts recovered from the treated animals.

In vivo activity of albendazole in combination with thymol against Echinococcus multilocularis

“…Even though interesting advances have been reported in the in vitro or in vivo application of several essential oils or its components on E. granulosus (Maggiore et al, 2012;Moazeni et al, 2014;Pensel et al, 2014), little is known regarding E. multilocularis.…”

Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice

“…All mice (from groups , and were euthanized at the end the experiment (after 6 months) by Ether, and a necropsy was carried out immediately. At necropsy, the body cavity was opened, and the internal organs were observed for hydatid cysts (11).…”

“…Formalin, hypertonic saline, cetrimide, chlorhexidine, hydrogen peroxide and ethyl alcohol are some of the compounds used as scolicidal, but all of these agents are concentration dependent and their degree of dilution in the cyst contents is quite unpredictable (10), Also, they have different adverse effects such as liver necrosis and biliary tract fibrosis (2). Thus, it is indispensable to develop new scolicidal materials with more efficacious and low adverse effects, which can be implemented during surgery (11). Considerable evidence has demonstrated that some plant derived products (PDPs) can be useful as scolicidal (12).…”

“…Considerable evidence has demonstrated that some plant derived products (PDPs) can be useful as scolicidal (12). Based on previous in vitro and in vivo data on scolicidal effects of PDPs, Mallotus philippinensis (13), Allium sativum (Garlic) (6), Zingiber (14), Zataria multiflora (11) and Berberis vulgaris (15) showed significant scolicidal activity. Cucurbita maxima (Pumpkin) (Family Cucurbitaceae) commonly known as Squash is widely used as vegetable and a source of vitamin A, iron, phosphorus and calcium (16).…”

Influence of hydatid disease on the pregnancy outcomes: An experimental study

“…The larval stage of E. granulosus causes cystic echinococcosis (CE) (hydatid disease) in humans and livestock that represents an important zoonosis with worldwide distribution. Human infection usually occurs after ingestion of infective eggs passed in the feces from dogs via direct contact or through environmental contamination . CE is a chronic disease with complex nature and may be asymptomatic, severe or even a fatal infection in human beings .…”