In Vivo Study on Site of Action of Sinapine Thiocyanate following Acupoint Herbal Patching

Abstract

Objective To investigate the site of action of sinapine thiocyanate (ST), following acupoint herbal patching (AHP). Methods Twenty Wistar rats were randomized into five groups (groups A, B, C, D, and E), and all groups received the same AHP in vivo. Skin samples were excised at 2 h, 4 h, 6 h, 10 h, and 26 h after AHP administration from group A to group E separately and the concentrations of ST in the skin were determined using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method. A pharmacokinetic profile of ST following AHP was performed at the same time in a group of five Wistar rats to detect plasma levels at the same time intervals. Results The mean ± SD ST concentrations (ng/ml) at 2 h (group A), 4 h (group B), 6 h (group C), 10 h (group D), and 26 h (group E) after AHP administration were 250.01 ± 61.99, 61.01 ± 30.41, 40.12 ± 26.94, 78.66 ± 59.43, and 19.55 ± 18.95, respectively. No ST was detected in rats' plasma samples at the same time points. Conclusions The site of action of ST following AHP is in the skin.

Sinapine thiocyanate alleviates intervertebral disc degeneration by not regulating JAK1/STAT3/NLRP3 signal pathway

“…26 Sinapine thiocyanate has a variety of biological activities, including anti-oxidant, anti-inflammatory, anti-radiation, anti-aging, anti-hypertensive, and anti-androgen activity, while also inhibiting angiogenesis. [27][28][29][30] It is reported that ST could also reduce cholesterol and low-density lipoprotein (LDL), significantly improve hypertension symptoms, prevent thrombosis, and attenuate thrombosis caused by inflammatory injury of vascular endothelial cells (VECs). 31 Sinapine thiocyanate improved vascular endothelial injury in hypertensive rats by inhibiting the activation of NLRP3 and the expression of related inflammatory factors (IL-1β and IL-18), and also alleviated human umbilical vein endothelial cells (HUVEC) injury induced by the administration of angiotensin II.…”

Effect of summer acupoint application treatment (SAAT) on gut microbiota in healthy Asian adults: A randomized controlled trial

“…[18,19] Therefore, AAT with this formula may contribute to the prevention of inflammatory and metabolic diseases. [20] Additionally, Cortex Cinnamomi and Flos Caryophylli are often applied to alleviate painful chronic diseases. [21,22] Correspondingly, the main acupuncture points used in combination include the Bladder meridian (BL), Ren meridian, and Du meridian according to clinical syndrome differentiation.…”