<i>In vivo</i>thickness measurement of basal cell carcinoma and actinic keratosis with optical coherence tomography and 20-MHz ultrasound

Mogensen, Mette; Nürnberg, Birgit Meinecke; Forman, Julie Lyng; Thomsen, Jakob Borup; Thrane, Lars; Jemec, Gregor B. E.

doi:10.1111/j.1365-2133.2008.09003.x

Cited by 133 publications

(163 citation statements)

References 44 publications

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“…Evaluation of the penetration depth as baseline value for therapy monitoring was significantly better with 50 MHz and higher than with 20 MHz. In a current study by Mogensen et al [4] an overestimation of the histological penetration depth in comparison of OCT and ultrasound was stated. This was, however, even more distinctive in OCT than in ultrasound.…”

Section: Resultsmentioning

confidence: 51%

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Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases

König

Speicher²,

Köhler

et al. 2010

Journal of Biophotonics

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Journal of BIOPHOTONICSThe first-ever application of high-frequency ultrasound combined with multiphoton tomography (MPT) and dermoscopy in a clinical trial is reported. 47 patients with different dermatoses such as benign and malign skin cancers, connective tissue diseases, inflammatory skin diseases, and autoimmune bullous skin diseases have been investigated with (i) state-of-the-art and highly sophisticated ultrasound systems for dermatology, (ii) the femtosecond laser multiphoton tomograph and (iii) dermoscopes. Dermoscopy provides two-dimensional color images of the skin surface with a magnification up to 70Â. Depending on the ultrasonic frequencies from 7.5 MHz to 100 MHz, the signal depth varies from about 1 mm to 80 mm. Vertical ultrasound wide-field images provide fast information on depth and volume of the lesion. The 100 MHz ultrasound allows imaging with resolutions down to 16 mm (axial) and 32 mm (lateral). Multiphoton tomography provides 0.36 Â 0.36 Â 0.001 mm 3 horizontal optical sections of a particular region of interest with submicron resolution down to 200 mm tissue depth. The autofluorescence of mitochondrial coenzymes, keratin, melanin, and elastin as well as the network of collagen structures can be imaged. The combination of ultrasound and MPT opens novel synergistic possibilities in diagnostics of skin diseases with a special focus on the early detection of skin cancer as well as the evaluation of treatments.Screenshot of human skin of the forearm. For a better visualization the A-scans at different depths are encoded in a 2D B-scan where the brightness represents the amplitudes.

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Section: Resultsmentioning

confidence: 51%

“…US has been used in the diagnosis of skin tumors, connective tissue diseases, vascular disorders, and subcutaneous processes [3] as well as to measure the thickness of skin [4][5][6][7][8]. A reliable differentiation between benign melanocytic nevi [9], and melanomas is not possible with clinical ultrasound [10].…”

Section: Introductionmentioning

confidence: 99%

Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases

König

Speicher²,

Köhler

et al. 2010

Journal of Biophotonics

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“…All nonmelanoma skin cancer lesions appeared hypoechogenic in HFUS, suggesting that this method alone is not suitable from differential diagnosis. [44][45][46] However, it gives a clear picture of the size and depth of the tumor. The method should be used as a complementary method in preoperative evolution of the tumor.…”

Section: High Frequency Ultrasonographymentioning

confidence: 99%

Non-invasive diagnostic techniques in the diagnosis of squamous cell carcinoma

Warszawik-Hendzel

Olszewska

Maj

et al. 2015

J Dermatol Case Rep

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Squamous cell carcinoma is the second most common cutaneous malignancy after basal cell carcinoma. Although the gold standard of diagnosis for squamous cell carcinoma is biopsy followed by histopathology evaluation, optical non-invasive diagnostic tools have obtained increased attention. Dermoscopy has become one of the basic diagnostic methods in clinical practice. The most common dermoscopic features of squamous cell carcinoma include clustered vascular pattern, glomerular vessels and hyperkeratosis. Under reflectance confocal microscopy, squamous cell carcinoma shows an atypical honeycomb or disarranged pattern of the spinous-granular layer of the epidermis, round nucleated bright cells in the epidermis and round vessels in the dermis. High frequency ultrasound and optical coherence tomography may be helpful in predominantly in pre-surgical evaluation of tumor size. Emerging non-invasive or minimal invasive techniques with possible application in the diagnosis of squamous cell carcinoma of the skin, lip, oral mucosa, vulva or other tissues include high-definition optical coherence tomography, in vivo multiphoton tomography, direct oral microscopy, electrical impedance spectroscopy, fluorescence spectroscopy, Raman spectroscopy, elastic scattering spectroscopy, differential path-length spectroscopy, nuclear magnetic resonance spectroscopy, and angle-resolved low coherence interferometry. (J Dermatol Case Rep. 2015; 9(4): 89-97)

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“…NMSC includes keratinocyte carcinomas, the most prevalent cancer type in humans, and some more rare skin cancers. In NMSC diagnosis, OCT can potentially reduce the number of invasive skin biopsies, assist in finding an optimal location for a biopsy or measure tumor thickness [206]. However, despite many promising initial results in research, OCT has not yet been established in clinical dermatology.…”

Section: Dermatologymentioning

confidence: 99%

Optical coherence tomography—current technology and applications in clinical and biomedical research

et al. 2011

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Optical coherence tomography (OCT) is a non-invasive imaging technique providing real-time twoand three-dimensional images of scattering samples with micrometer resolution. Mapping the local reflectivity, OCT visualizes the morphology of the sample. In addition, functional properties such as birefringence, motion or the distribution of certain substances can be detected with high spatial resolution. The main field of application is bio-medical imaging and diagnostics. In ophthalmology, OCT is accepted as a clinical standard for diagnosing and monitoring treatment of a number of retinal diseases, and OCT is becoming an important instrument for clinical cardiology. New applications are emerging in various medical fields, e. g. early-stage cancer detection, surgical guidance, and early diagnosis of musculoskeletal diseases. OCT has also proven its value as a tool for developmental biology. The number of companies involved in manufacturing OCT systems has increased substantially during the last years-especially due to its success in opthalmology-and this technology can be expected to continue to spread into various fields of application.

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In vivothickness measurement of basal cell carcinoma and actinic keratosis with optical coherence tomography and 20-MHz ultrasound

Abstract: OCT appears more precise and less biased than HFUS for thickness measurement in AK and BCC lesions < 2 mm, but both OCT and especially HFUS tended to overestimate tumour thickness.

Cited by 133 publications

References 44 publications

Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases

Clinical application of multiphoton tomography in combination with high‐frequency ultrasound for evaluation of skin diseases

Non-invasive diagnostic techniques in the diagnosis of squamous cell carcinoma

Optical coherence tomography—current technology and applications in clinical and biomedical research

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