<i>In vivo</i>
            tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel

Rossi, Claudia; Flaibani, Marina; Blaauw, Bert; Pozzobon, Michela; Figallo, Elisa; Reggiani, Carlo; Vitiello, Libero; Elvassore, Nicola; Coppi, Paolo De

doi:10.1096/fj.10-174755

Cited by 163 publications

(158 citation statements)

References 33 publications

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“…Single myofibers were isolated from BM-and AFS-transplanted animals and from C57BL/6 GFP þ mice, as previously described [12,21]. Briefly, extensor digitorum longus (EDL) and Soleus muscle were digested for 2 hours at 37 C in 0.2% (wt/vol) type I collagenase (Sigma-Aldrich), reconstituted in DMEM (high glucose, with L-glutamine, supplemented with 1% penicillin-streptomycin; Invitrogen).…”

Section: Secondary Transplantsmentioning

confidence: 99%

“…4A). Single myofibers were isolated from BM-(n ¼ 6) and AFS- (n ¼ 6) transplanted animals and from WT C57BL/6 GFP þ mice (n ¼ 3), as previously described [12,21]. Five hundred freshly isolated SCs, disengaged from single myofibers, were injected locally into TA muscles of naive, 3-month-old HSACre, Smn F7/F7 mice.…”

Section: Afs Cells Engraft In the Muscle Stem Cell Nichementioning

confidence: 99%

“…They can also generate myotubes in vitro [1], but very little is known about their potential to functionally engraft into regenerating skeletal muscle [9]. So far, freshly isolated satellite cells (SCs), which are postnatally responsible of both de novo muscle formation and repair, showed the best myogenic potential, even if they are limited in number and do not engraft when injected systemically [10][11][12]. Although alternative cell sources have been explored, replacement of stem cells in damaged or diseased skeletal muscles remains an unsolved problem.…”

Section: Introductionmentioning

confidence: 99%

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Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

et al. 2012

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Mutations in the survival of motor neuron gene (SMN1) are responsible for spinal muscular atrophy, a fatal neuromuscular disorder. Mice carrying a homozygous deletion of Smn exon 7 directed to skeletal muscle (HSA-Cre, Smn F7/F7 mice) present clinical features of human muscular dystrophies for which new therapeutic approaches are highly warranted. Herein we demonstrate that tail vein transplantation of mouse amniotic fluid stem (AFS) cells enhances the muscle strength and improves the survival rate of the affected animals. Second, after cardiotoxin injury of the Tibialis Anterior, only AFS-transplanted mice efficiently regenerate. Most importantly, secondary transplants of satellite cells (SCs) derived from treated mice show that AFS cells integrate into the muscle stem cell compartment and have long-term muscle regeneration capacity indistinguishable from that of wild-type-derived SC. This is the first study demonstrating the functional and stable integration of AFS cells into the skeletal muscle, highlighting their value as cell source for the treatment of muscular dystrophies.

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Section: Secondary Transplantsmentioning

confidence: 99%

Section: Afs Cells Engraft In the Muscle Stem Cell Nichementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

et al. 2012

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“…3,4 While several important studies. [5][6][7] have focused on developing methods to improve survival, neovascularization, and functional integration of engineered muscle tissues in vivo, the force generating capacity of skeletal muscle constructs still remains significantly inferior to that of the native muscle. 8,9 Thus, methods to enhance the contractile function of engineered muscle constructs are expected to promote the design of efficient muscle tissue engineering therapies and better 3D tissue models for basic studies of muscle biology and function.…”

Section: Introductionmentioning

confidence: 99%

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

Bian

Juhas

Pfeiler

et al. 2012

Tissue Engineering Part A

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The field of skeletal muscle tissue engineering is currently hampered by the lack of methods to form large muscle constructs composed of dense, aligned, and mature myofibers and limited understanding of structure-function relationships in developing muscle tissues. In our previous studies, engineered muscle sheets with elliptical pores (''muscle networks'') were fabricated by casting cells and fibrin gel inside elastomeric tissue molds with staggered hexagonal posts. In these networks, alignment of cells around the elliptical pores followed the local distribution of tissue strains that were generated by cell-mediated compaction of fibrin gel against the hexagonal posts. The goal of this study was to assess how systematic variations in pore elongation affect the morphology and contractile function of muscle networks. We found that in muscle networks with more elongated pores the force production of individual myofibers was not altered, but the myofiber alignment and efficiency of myofiber formation were significantly increased yielding an increase in the total contractile force despite a decrease in the total tissue volume. Beyond a certain pore length, increase in generated contractile force was mainly contributed by more efficient myofiber formation rather than enhanced myofiber alignment. Collectively, these studies show that changes in local tissue geometry can exert both direct structural and indirect myogenic effects on the functional output of engineered muscle. Different hydrogel formulations and pore geometries will be explored in the future to further augment contractile function of engineered muscle networks and promote their use for basic structure-function studies in vitro and, eventually, for efficient muscle repair in vivo.

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“…While debates have been generated around which myogenic progenitors (satellite cells, muscle stem cells, or myoblasts) should be used, fewer studies have focused on exploring which matrix could offer a better platform for regeneration (10,11).…”

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confidence: 99%

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

Fishman

Lowdell

Urbani³

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Decellularized (acellular) scaffolds, composed of natural extracellular matrix, form the basis of an emerging generation of tissueengineered organ and tissue replacements capable of transforming healthcare. Prime requirements for allogeneic, or xenogeneic, decellularized scaffolds are biocompatibility and absence of rejection. The humoral immune response to decellularized scaffolds has been well documented, but there is a lack of data on the cellmediated immune response toward them in vitro and in vivo. Skeletal muscle scaffolds were decellularized, characterized in vitro, and xenotransplanted. The cellular immune response toward scaffolds was evaluated by immunohistochemistry and quantified stereologically. T-cell proliferation and cytokines, as assessed by flow cytometry using carboxy-fluorescein diacetate succinimidyl ester dye and cytometric bead array, formed an in vitro surrogate marker and correlate of the in vivo host immune response toward the scaffold. Decellularized scaffolds were free of major histocompatibility complex class I and II antigens and were found to exert anti-inflammatory and immunosuppressive effects, as evidenced by delayed biodegradation time in vivo; reduced sensitized T-cell proliferative activity in vitro; reduced IL-2, IFN-γ, and raised IL-10 levels in cell-culture supernatants; polarization of the macrophage response in vivo toward an M2 phenotype; and improved survival of donor-derived xenogeneic cells at 2 and 4 wk in vivo. Decellularized scaffolds polarize host responses away from a classical TH1-proinflammatory profile and appear to down-regulate T-cell xeno responses and TH1 effector function by inducing a state of peripheral T-cell hyporesponsiveness. These results have substantial implications for the future clinical application of tissue-engineered therapies.

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In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel

Cited by 163 publications

References 33 publications

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

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In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel

Cited by 163 publications

References 33 publications

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn F7/F7 Mouse Model

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn F7/F7 Mouse Model

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

Immunomodulatory effect of a decellularized skeletal muscle scaffold in a discordant xenotransplantation model

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Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model

Amniotic Fluid Stem Cells Restore the Muscle Cell Niche in a HSA‐Cre , Smn ^F7/F7 Mouse Model