<i>In vivo</i> toxicity evaluation of nanoemulsions for drug delivery

Hort, Mariana Appel; Alves, Barbara da Silva; Júnior, Osmar Vieira Ramires; Falkembach, Mariana; Araújo, Gabriela de Moraes Soares; Fernandes, Caroline Lopes Feijó; Tavella, Ronan Adler; Bidone, Juliana; Dora, Cristiana Lima; Júnior, Flávio Manoel Rodrigues da Silva

doi:10.1080/01480545.2019.1659806

Cited by 15 publications

(5 citation statements)

References 53 publications

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“…To identify micronuclei the criteria were: (1) they contained a rounded smooth perimeter suggestive of a membrane; (2) micronuclei were less than a third the diameter of the associated nucleus, but large enough to discern shape and color; (3) staining intensity was similar to that of the nucleus; (4) texture was similar to that of the nucleus; (5) they were in the same focal plane as the nucleus; and (6) an absence of overlap with, or bridge to, the nucleus. Furthermore, numerous studies of the research group have already been published with the micronucleus technique: in rodent models (da Silva Júnior et al, 2013a, 2013b; Garcia et al, 2016, 2017; Hort et al, 2019); amphibians (Josende et al, 2015); earthworms (da Silva Júnior et al, 2019); and humans (da Silva Júnior et al, 2018; da Silva Pinto et al, 2017). Finally, it is worth mentioning two other concerns addressed by our group to minimize possible errors or biases in our analyses: (1) the slides were photographed for, if necessary, a subsequent reinterpretation and (2) the entire reading of the slides was done blindly (each individual received a numeric code and the analyzer was unaware of the participant’s allocation within the groups).…”

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confidence: 99%

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

et al. 2020

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We would like to thank the journal for the opportunity to respond to the issues raised in the letter to the editor "Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate" and to clarify aspects of our methodology in relation to these concerns. We would also like to thank the letter's authors for their interest in our work and for taking the time to express their valuable points of view. First, in their letter to the editor, the authors raise concerns about the staining procedure used. In this regard, we agree that the use of stains like Giemsa in oral mucosa cells has been questioned by researchers like Bonassi et al. (2011) (the study referenced by the letter's authors); however, this study does not bring comparative results with the use of eosin methylene blue according to Leishman. In fact, the same study does not rule out the use of simple staining techniques, such as May-Grünwald. Even so, recent studies, as mentioned by Antoniazzi et al. (2018) and other studies (Vilas Boas et al., 2018), showed promising and reliable results with the Giemsa staining technique. The staining procedure used in the study by da Silva Júnior et al. (2020), a solution of eosin methylene blue according to Leishman, is an appropriate cocktail for differential visualization of both cytoplasm and cell nucleus. Korsakov et al. (2015) used the solution of eosin methylene blue according to Leishman for staining oral cells in their study that recommended the use of oral epithelium cells as an environmental indicator.

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confidence: 99%

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

et al. 2020

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“…In a report, size and function of animal organs were not influenced by NE administration, while hematological and biochemical parameters changed dose‐dependently. Also, at similar concentrations, oral administration showed lower toxic effects than the intraperitoneal route (Hort et al, 2021).…”

Section: Safetymentioning

confidence: 97%

Current challenges ahead in preparation, characterization, and pharmaceutical applications of nanoemulsions

Yousefpoor,

Esnaashari,

Baharifar

et al. 2023

WIREs Nanomed Nanobiotechnol

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Nanoemulsions (NEs) are emulsions with particle size of less than around 100 nm. Reviewing the literature, several reports are available on NEs, including preparation, characterization, and applications of them. This review aims to brief challenges that researchers or formulators may encounter when working with NEs. For instance, when selecting NE components and identifying their concentrations, stability and safety of the preparation should be evaluated. When preparing an NE, issues over scale‐up of the preparation as well as possible effects of the preparation process on the active ingredient need to be considered. When characterizing the NEs, the two major concerns are accuracy of the method and accessibility of the characterizing instrument. Also a highly efficient NE for clinical use to deliver the active ingredient to the target tissue with maximum safety profile is commonly sought. Throughout the review we also have tried to suggest approaches to overcome the challenges.This article is categorized under: Therapeutic Approaches and Drug Discovery > Emerging Technologies

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“…In a recent study, Hort et al [101] evaluated the toxicity of Miglyol and egg lecithin nanoemulsions using an in vivo model (male Wistar rats). The nanoemulsions were orally administered to rats for 21 days at lipid concentrations of 200, 400, or 800 mg/kg of body weight.…”

Section: Potential Toxicity Limitations and Regulatory Aspects Of Nanoemulsionsmentioning

confidence: 99%

Nanoemulsions as Edible Coatings: A Potential Strategy for Fresh Fruits and Vegetables Preservation

Filho

Miranda

Ferreira

et al. 2021

Foods

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Fresh fruits and vegetables are perishable commodities requiring technologies to extend their postharvest shelf life. Edible coatings have been used as a strategy to preserve fresh fruits and vegetables in addition to cold storage and/or controlled atmosphere. In recent years, nanotechnology has emerged as a new strategy for improving coating properties. Coatings based on plant-source nanoemulsions in general have a better water barrier, and better mechanical, optical, and microstructural properties in comparison with coatings based on conventional emulsions. When antimicrobial and antioxidant compounds are incorporated into the coatings, nanocoatings enable the gradual and controlled release of those compounds over the food storage period better than conventional emulsions, hence increasing their bioactivity, extending shelf life, and improving nutritional produce quality. The main goal of this review is to update the available information on the use of nanoemulsions as coatings for preserving fresh fruits and vegetables, pointing to a prospective view and future applications.

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In vivo toxicity evaluation of nanoemulsions for drug delivery

Cited by 15 publications

References 53 publications

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

Current challenges ahead in preparation, characterization, and pharmaceutical applications of nanoemulsions

Nanoemulsions as Edible Coatings: A Potential Strategy for Fresh Fruits and Vegetables Preservation

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