<i>In Vivo</i>Transplantation of Autogenous Marrow-Derived Cells Following Rapid Intraoperative Magnetic Separation Based on Hyaluronan to Augment Bone Regeneration

Caralla, Tonya; Joshi, Powrnima; Fleury, Sean; Luangphakdy, Viviane; Shinohara, K.; Pan, Hui; Boehm, Cynthia; Vasanji, Amit; Hefferan, Theresa E.; Walker, Esteban; Yaszemski, Michael J.; Hascall, Vincent C.; Zborowski, Maciej; Muschler, George F.

doi:10.1089/ten.tea.2011.0622

Cited by 32 publications

(42 citation statements)

References 27 publications

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“…This premise is supported by the findings of Takigami et al (2007) that were discussed above. It is also supported by the recent findings of Caralla et al (2013), who used the CFMD model to evaluate the efficacy of transplantation of marrow-derived cells that had been processed using magnetic separation to concentrate and select osteogenic connective tissue progenitors using a novel marker (hyaluronan). They found that transplantation of this enriched population resulted in increased new bone formation and increased vascularity when compared to unprocessed marrow.…”

Section: Discussionmentioning

confidence: 68%

“…As such, the surface of the graft site will contain both a source of oxygen via diffusion and a source of both angiogenic and osteogenic cells. This environment enables the assessment of the relative osteoconductive effect of scaffold materials that are placed into the defect (Caralla et al, 2013;. This environment also enables the assessment of the biological effect of an implant containing bioactive molecules such as rhBMP-2, because the implant site contains a population of local progenitors which serves as a target population on which the bioactive agent may act (Takigami et al, 2007 ).…”

Section: Discussionmentioning

confidence: 99%

“…rhBMP-2/collagen/TCP evaluation in a canine defect the CFMD model, where bone formation at four weeks following implantation was found to be greatest and most mature at the periphery and extended to various degrees deeper into the implantation site (Caralla et al, 2013;Takigami et al, 2007). The explanation for this finding is uncertain.…”

Section: Discussionmentioning

confidence: 99%

“…BMA has also been used for decades as a source of transplantable osteogenic connective tissue progenitors (CTP-Os) . Several uncontrolled clinical series support the clinical value of aspirated marrow (Caralla et al, 2013;Muschler et al, 1993;. Connolly and coworkers reported successful clinical treatment of 20 non-unions, and went on to show that concentrating marrow-derived cells using intraoperative processing might be used to improve outcome (Connolly et al, 1989;Connolly et al, 1991;Tiedeman et al, 1991).…”

Section: Evaluation Of Rhbmp-2/collagen/tcp-ha Bone Graft With and Wimentioning

confidence: 99%

“…This study was designed to characterize the performance of a bone graft comprising rhBMP-2/ACS in which the sponge is wrapped around tri-calcium phosphate-hydroxyapatite (TCP-HA) granules (rhBMP-2/ ACS/TCP-HA) and to test the hypothesis that addition of BMA will improve the performance of this construct in the established Canine Femoral Multi Defect (CFMD) Model (Caralla et al, 2013;Takigami et al, 2007).…”

Section: Evaluation Of Rhbmp-2/collagen/tcp-ha Bone Graft With and Wimentioning

confidence: 99%

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Evaluation of rhBMP-2/collagen/TCP-HA bone graft with and without bone marrow cells in the canine femoral multi defect model

Luangphakdy

Shinohara

Pan

et al. 2015

eCM

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Recombinant human bone morphogenetic protein-2, when applied to an absorbable type 1 bovine collagen sponge (rhBMP-2/ACS) is an effective therapy in many bone grafting settings. Bone marrow aspirate (BMA) has also been used as a source of transplantable osteogenic connective tissue progenitors. This study was designed to characterize the performance of a scaffold comprising rhBMP-2/ACS in which the sponge wraps around tricalcium phosphate hydroxyapatite granules (rhBMP-2/ ACS/TCP-HA) and to test the hypothesis that addition of BMA will improve the performance of this construct in the Canine Femoral Multi Defect Model. In each subject, two sites were grafted with rhBMP-2/ACS/TCP-HA scaffold loaded with BMA clot and two other sites with rhBMP-2/ACS/TCP-HA scaffold loaded with wound blood (WB). After correction for unresorbed TCP-HA granules, sites grafted with rhBMP-2/ACS/TCP-HA+BMA and rhBMP-2/ACS/TCP-HA+WB were similar, with mean percent bone volumes of 10.9 % ± 1.2 and 11.2 % ± 1.2, respectively. No differences were seen in quantitative histomorphometry. While bone formation using both constructs was robust, this study did not support the hypothesis that the addition of unprocessed bone marrow aspirate clot improved bone regeneration in a site engrafted with rhBMP-2/ACS/TCP-HA+BMA. In contrast to prior studies using this model, new bone formation was greater at the center of the defect where TCP-HA was distributed. This finding suggests a potential synergy between rhBMP-2 and the centrally placed ceramic and cellular components of the graft construct. Further optimization may also require more uniform distribution of TCP-HA, alternative cell delivery strategies, and a more rigorous large animal segmental defect model.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Evaluation Of Rhbmp-2/collagen/tcp-ha Bone Graft With and Wimentioning

confidence: 99%

Section: Evaluation Of Rhbmp-2/collagen/tcp-ha Bone Graft With and Wimentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of rhBMP-2/collagen/TCP-HA bone graft with and without bone marrow cells in the canine femoral multi defect model

Luangphakdy

Shinohara

Pan

et al. 2015

eCM

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Magnetophoresis: Fundamentals and Applications

Zborowski

Chalmers

2015

Wiley Encyclopedia of Electrical and Electronics Engineering

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The term “magnetophoresis” has been proposed to describe the behavior of a magnetic particle moving through a viscous medium under the influence of an external magnetic field. Its origin can be traced to a similar term used to describe particle motion in a viscous medium under the influence of an electric field, “electrophoresis.” The number of publications on magnetophoresis has rapidly increased during the last 10 years primarily due to a burgeoning interest in its applications to biomedical research and clinical diagnostics and therapy. The appealing feature of magnetophoresis is that under well‐controlled conditions it provides a means for gentle cell separation in its natural milieu that could be both highly specific and highly sensitive at a relatively low cost with respect to the separation equipment. The increasing interest in single‐cell biology in health and disease and the rapidly improving sensitivity of molecular biology methods in applications to single‐cell genomic analysis put a special emphasis on high quality, high volume, and relatively inexpensive cell separation methods, such as those provided by cell magnetophoresis in microfluidic channels. The growth in magnetophoresis research and applications is aided by a synergy between materials science, nanoparticle synthesis and characterization, micro‐ and nanofluidic engineering, experimental and computational fluid dynamics research, and physics of high magnetic fields. This leads to emerging applications, such as detection of paramagnetic impurities in diamagnetic samples and biomagnetic cell separations. These are areas of magnetophoresis applications that will likely continue to rapidly grow.

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Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

Kaizawa

Franklin

Leyden

et al. 2019

Journal Orthopaedic Research

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Rotator cuff (RTC) repair outcomes are unsatisfactory due to the poor healing capacity of the tendon bone interface (TBI). In our preceding study, tendon hydrogel (tHG), which is a type I collagen rich gel derived from human tendons, improved biomechanical properties of the TBI in a rat chronic RTC injury model. Here we investigated whether adipose‐derived stem cell (ASC)‐seeded tHG injection at the repair site would further improve RTC healing. Rats underwent bilateral supraspinatus tendon detachment. Eight weeks later injured supraspinatus tendons were repaired with one of four treatments. In the control group, standard transosseous suture repair was performed. In the ASC, tHG, tHGASC groups, ASC in media, tHG, and ASC‐seeded tHG were injected at repair site after transosseous suture repair, respectively. Eight weeks after repair, the TBI was evaluated biomechanically, histologically, and via micro CT. Implanted ASCs were detected in ASC and tHGASC groups 7 weeks after implantation. ACS implantation improved bone morphometry at the supraspinatus insertion on the humerus. Injection of tHG improved biomechanical properties of the repaired TBI. RTC healing in tHGASC group was significantly better than control but statistically equivalent to the tHG group based on biomechanical properties, fibrocartilage area at the TBI, and bone morphometry at the supraspinatus insertion. In a rat RTC chronic injury model, no biomechanical advantage was gained with ASC augmentation of tHG. Clinical Significance: Tendon hydrogel augmentation with adipose derived stem cells does not significantly improve TBI healing over tHG alone in a chronic rotator cuff injury model. © 2019 Orthopaedic Research Society. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

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In VivoTransplantation of Autogenous Marrow-Derived Cells Following Rapid Intraoperative Magnetic Separation Based on Hyaluronan to Augment Bone Regeneration

Cited by 32 publications

References 27 publications

Evaluation of rhBMP-2/collagen/TCP-HA bone graft with and without bone marrow cells in the canine femoral multi defect model

Evaluation of rhBMP-2/collagen/TCP-HA bone graft with and without bone marrow cells in the canine femoral multi defect model

Magnetophoresis: Fundamentals and Applications

Augmentation of chronic rotator cuff healing using adipose‐derived stem cell‐seeded human tendon‐derived hydrogel

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