<i>In vivo</i>validation of quantitative frequency domain fluorescence tomography

Lin, Yuting; Ghijsen, Michael; Nalcioğlu, Orhan; Gülşen, Gültekin

doi:10.1117/1.jbo.17.12.126021

Cited by 7 publications

(5 citation statements)

References 37 publications

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“…FD measurements have been made in phantoms using gain-modulated intensified CCDs (Godavarty et al 2003, 2005, Joshi et al 2006, Ge et al 2008, Kim et al 2010 as well as modulated PMTs (Eppstein et al 2002, Lee andSevick-Muraca 2001). In animals, there have been two reports of fluorescence tomography from FD measurements using a gain-modulated ICCD (Darne et al 2012) and PMTs (Lin et al 2011(Lin et al , 2012.…”

Section: Frequency-domain Time-dependent Measurements For Tomographymentioning

confidence: 99%

“…Highlights of time-dependent and -independent fluorescence small animal tomography Despite time-dependent methods offering the most complicated instrumentation, key demonstration of their successful adaptation into conventional scanners have been shown to be feasible. In a study performed by Lin et al (2012), 100 MHz FD measurements based upon a network analyzer and single PMTs coupled to the animal surface (figure 12(a)) were used to reconstruct images of a tube containing 52 μmol of indocyanine green embedded in the abdomen of a rat. While it is unclear whether homodyne or heterodyne measurements were conducted, lifetime and absorption cross section of ICG was reconstructed (i) without a priori information, (ii) using fluorescence measurements normalized with measurements made at the incident excitation wavelengths, and (iii) using the endogenous optical property maps obtained from independent measurements made with DOT.…”

Section: Examples Of Implementation and Future Directionsmentioning

confidence: 99%

See 1 more Smart Citation

Small animal fluorescence and bioluminescence tomography: a review of approaches, algorithms and technology update

Darne¹,

Lu²,

2013

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Emerging fluorescence and bioluminescence tomography approaches have several common, yet several distinct features from established emission tomographies of PET and SPECT. Although both nuclear and optical imaging modalities involve counting of photons, nuclear imaging techniques collect the emitted high energy (100-511 keV) photons after radioactive decay of radionuclides while optical techniques count low-energy (1.5-4.1 eV) photons that are scattered and absorbed by tissues requiring models of light transport for quantitative image reconstruction. Fluorescence imaging has been recently translated into clinic demonstrating high sensitivity, modest tissue penetration depth, and fast, millisecond image acquisition times. As a consequence, the promise of quantitative optical tomography as a complement of small animal PET and SPECT remains high. In this review, we summarize the different instrumentation, methodological approaches and schema for inverse image reconstructions for optical tomography, including luminescence and fluorescence modalities, and comment on limitations and key technological advances needed for further discovery research and translation.

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Section: Frequency-domain Time-dependent Measurements For Tomographymentioning

confidence: 99%

Section: Examples Of Implementation and Future Directionsmentioning

confidence: 99%

Small animal fluorescence and bioluminescence tomography: a review of approaches, algorithms and technology update

Darne¹,

Lu²,

2013

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“…It also assumes that the imaged animal remains stationary as the quality of the corrected measurements using this method highly depends on the ability to immobilize the animal during this period between the two data acquisitions (Zhang et al 2018). Moreover, keeping the same experimental settings for both measurements is an additional challenge as gain settings are set only when the fluorescent agent is injected (Lin et al 2012a). In addition, when using targeted fluorescent probes that require a long circulation period, generally over 10 h, to accumulate in tumours, this method is useless due to the difficulty of replicating the exact same experimental settings to acquire before and after injection data (Ardeshirpour et al 2014(Ardeshirpour et al , 2018.…”

Section: Introductionmentioning

confidence: 99%

A thermo-sensitive fluorescent agent based method for excitation light leakage rejection for fluorescence molecular tomography

et al. 2019

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Fluorescence molecular tomography (FMT) is widely used in preclinical oncology research. FMT is the only imaging technique able to provide 3D distribution of fluorescent probes within thick highly scattering media. However, its integration into clinical medicine has been hampered by its low spatial resolution caused by the undetermined and ill-posed nature of its reconstruction algorithm. Another major factor degrading the quality of FMT images is the large backscattered excitation light component leaking through the rejection filters and coinciding with the weak fluorescent signal arising from a low tissue fluorescence concentration. In this paper, we present a new method based on the use of a novel thermo-sensitive fluorescence probe. In fact, the excitation light leakage is accurately estimated from a set of measurements performed at different temperatures and then is corrected for in the tomographic data. The obtained results show a considerable improvement in both spatial resolution and quantitative accuracy of FMT images due to the proper correction of fluorescent signals.

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“…Many studies have demonstrated that integrating structural a priori information derived from a high resolution anatomical imaging modality such as computed tomography (CT) and MRI can improve the resolution and quantitative accuracy of the recovered fluorescent source in bioluminescent (BLT) and fluorescent tomography (FT) [29–33]. In fact, functional a priori information derived from DOT has also been shown to improve image performance by estimating optical scattering and absorption coefficients [34,35]. In addition to improving the reconstruction by providing a priori information, integration with anatomical imaging modalities or non-traditional functional imaging modalities such as positron emission tomography (PET) and single-photon emission computed tomography (SPECT) can also provide a much needed cross-validation of the optical information [36–41].…”

Section: Introductionmentioning

confidence: 99%

Differentiation of tumor vasculature heterogeneity levels in small animals based on total hemoglobin concentration using magnetic resonance-guided diffuse optical tomography in vivo

et al. 2016

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Insight into the vasculature of the tumor in small animals has the potential to impact many areas of cancer research. The heterogeneity of the vasculature of a tumor is directly related to tumor stage and disease progression. In this small scale animal study, we investigated the feasibility of differentiating tumors with different levels of vasculature heterogeneity in vivo using a previously developed hybrid magnetic resonance imaging (MRI) and diffuse optical tomography (DOT) system for small animal imaging. Cross-sectional total hemoglobin concentration maps of 10 Fisher rats bearing R3230 breast tumors are reconstructed using multi-wavelength DOT measurements both with and without magnetic resonance (MR) structural a priori information. Simultaneously acquired MR structural images are used to guide and constrain the DOT reconstruction, while dynamic contrast-enhanced MR functional images are used as the gold standard to classify the vasculature of the tumor into two types: high versus low heterogeneity. These preliminary results show that the stand-alone DOT is unable to differentiate tumors with low and high vascular heterogeneity without structural a priori information provided by a high resolution imaging modality. The mean total hemoglobin concentrations comparing the vasculature of the tumors with low and high heterogeneity are significant (p-value 0.02) only when MR structural a priori information is utilized.

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In vivovalidation of quantitative frequency domain fluorescence tomography

Cited by 7 publications

References 37 publications

Small animal fluorescence and bioluminescence tomography: a review of approaches, algorithms and technology update

Small animal fluorescence and bioluminescence tomography: a review of approaches, algorithms and technology update

A thermo-sensitive fluorescent agent based method for excitation light leakage rejection for fluorescence molecular tomography

Differentiation of tumor vasculature heterogeneity levels in small animals based on total hemoglobin concentration using magnetic resonance-guided diffuse optical tomography in vivo

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