<i>Interleukin 10</i> gene promoter polymorphisms are associated with chronic periodontitis

Scarel‐Caminaga, Raquel Mantuaneli; Trevilatto, Paula Cristina; Souza, Ana Paula de; Brito, Rui Barbosa de; Camargo, L. E. A.; Line, Sérgio Roberto Peres

doi:10.1111/j.1600-051x.2004.00500.x

Cited by 111 publications

(139 citation statements)

References 23 publications

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“…Regarding the IL-10 polymorphism, in contrast to other studies (15,(22)(23)(24), an association between allele A and either CP or AP was not observed in our study. A recent meta-analysis suggests that the IL-10 -597 An allele can provide an increased CP risk compared with the C allele.…”

Section: Genotypes and Alleles Distribution Of Polymorphisms In The Ncontrasting

confidence: 55%

“…However, the presence of an association between IL-10 gene polymorphisms and clinical manifestations of chronic and aggressive periodontitis is controversial. Some of the studies failed to find any evidence of association (4,19,20) and others revealed a possible association between this polymorphism and periodontitis (17,(21)(22)(23)(24). Considering the multifactorial nature of a periodontal disease, little is known among the complex interaction of the polymorphisms in the promoter regions of IL-6 (-174), IL-10 (-597) and NF-κB1 (-94ins/del) genes and clinical parameters of periodontitis.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Toker¹,

Görgün²,

Korkmaz³

2017

Cent Eur J Public Health

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SUMMARYObjective: Pro-inflammatory cytokines, interleukin-6 (IL-6), demonstrated to be suppressed by interleukin-10 (IL-10) are known to be regulated by the transcription factor nuclear factor-κB(NF-κB). The aim of this study was to ascertain the association between genetic polymorphism of these genes (IL-6(-174), IL-10(-597) and NF-κB1-94ins/del)) and chronic/aggressive periodontitis.Methods: Forty-five patients with chronic periodontitis (CP), 58 patients with aggressive periodontitis (AP) and 38 periodontally healthy subjects were included in this study. Genomic DNA was isolated from whole blood samples. The NF-κB, IL-6, and IL-10 polymorphisms were determined by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method.Results: Among subjects for the ins/ins genotypes of NF-κB1 gene, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis group than in healthy controls (p = 0.023). A statistically significant difference in genotyping frequencies between AP group and healthy controls was observed for the IL-6 gene. The AA genotype of IL-10 was overrepresented in CP and AP groups compared to healthy controls (OR = 9.93, 95% CI: 2.11-46.7, OR = 5.7, 95% CI: 1.22-26.89, respectively).Conclusions: Within the limits of this study, it can be concluded that the IL-10 (-597) AA genotype is associated with susceptibility to chronic/ aggressive periodontitis and IL-6 (-174) GG genotypes and G allele seems to be associated with aggressive periodontitis.Clinical relevance: The results of the current study indicate that IL-6 and IL-10 genotypes seem to be associated with aggressive periodontitis. Also, the AA genotypes of IL-10 presented a higher frequency in chronic periodontitis subjects with carrying NF-κB1 ins/ins genotypes.

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Section: Genotypes and Alleles Distribution Of Polymorphisms In The Ncontrasting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Toker¹,

Görgün²,

Korkmaz³

2017

Cent Eur J Public Health

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“…SNPs in the IL-10 gene have been associated with altered synthesis of IL-10 in response to inflammatory stimuli that could be detrimental to host tissues and could be linked to periodontal disease susceptibility. 41,42 IL-4 Polymorphism:-IL-4 is a potent down regulator of macrophage function inhibiting the secretion of PGE2 and cytokines by macrophages. Furthermore IL-4 can also down-regulate the CD14 receptor and is also found to induce apoptosis in monocytes.…”

Section: Il-10 Polymorphism:-mentioning

confidence: 99%

Genetic Background in Initiation, Progression and Treatment of Periodontal Diseases – A Review.

RagaBindu¹,

Vidyasagar²,

P³

et al. 2017

IJAR

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“…Indeed, recent studies have shown that single nucleotide polymorphisms (SNPs) in genes such as interleukin (IL)-2, IL-6, IL-10, matrix metalloprotease 1, and tumor necrosis factor receptor (TNFR) 2 are associated with periodontal disease [6][7][8][9][10] . We also previously showed that SNP-frequencies of the IL-13 gene are associated with periodontitis in the Japanese population 11) , but not for those of the IL-1A, IL-1B, toll-like receptor (TLR) 4, TLR2, and histatin genes 12,13) .…”

Section: Introductionmentioning

confidence: 99%

A Novel Single Nucleotide Polymorphism of the Interleukin-8 Promoter: Its Transcriptional Regulation and Analysis of the Mutation in Periodontal Disease in the Japanese Population

Imamura

Fujigaki

Higaki

et al. 2012

J. Hard Tissue Biology.

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Abstract：Chronic periodontitis is a major dental disease associated with continuous inflammation and the mediation of cytokines and chemokines such as Interleukin (IL)-8. Periodontitis processes may also be associated with quantitative changes in IL-8 gene expression. We examined the transcriptional activation and mediation of a novel single nucleotide polymorphism (SNP) at position -845 (from the transcription initiation site) of the IL8 promoter. Moreover, we investigated whether the frequencies of this SNP were associated with susceptibility to chronic periodontitis among Japanese. The fragments of cloned IL8 promoter (-866 to +30) containing the novel SNP (-845T and -845C) were ligated to the luciferase gene. A luciferase assay was then conducted using these reporter plasmids in SCCTM cells. The presence and molecular weight of the -845T-specific binding proteins were analyzed by gel mobility shift and UV-crosslinking competition assays, respectively. SNP analysis was performed with DNA from 56 control subjects and 52 patients by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and disease associations were analyzed by a Chi-square test. These results indicate that transcriptional activity of the IL8 promoter containing -845T was approximately 9-fold higher than when the promoter contained -845C. -845T-specific binding proteins (approximately 55 and 60 kDa) were present in SCCTM cells. Genotype frequencies (T/T and T/C) between the control and patient groups were not significantly different (P = 0.586). These results suggest that transcription of the IL8 gene, particularly mediation of -845T/C, appears to be strictly regulated. This SNP was not associated with chronic periodontitis in the Japanese population.

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Interleukin 10 gene promoter polymorphisms are associated with chronic periodontitis

Abstract: Specific haplotypes and SNPs in IL10 gene are associated with susceptibility to CP in Brazilian patients.

Cited by 111 publications

References 23 publications

Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Analysis of IL-6, IL-10 and NF-κB Gene Polymorphisms in Aggressive and Chronic Periodontitis

Genetic Background in Initiation, Progression and Treatment of Periodontal Diseases – A Review.

A Novel Single Nucleotide Polymorphism of the Interleukin-8 Promoter: Its Transcriptional Regulation and Analysis of the Mutation in Periodontal Disease in the Japanese Population

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