ITGA4 Gene Methylation Status in Chronic Lymphocytic Leukemia

Abstract

Background: We aimed to investigate ITGA4 gene expression pattern and to explore its methylation heterogeneity in chronic lymphocytic leukemia (CLL). Patients & methods: Eighty one CLL patients and 75 healthy subjects were enrolled and prognostic evaluation of patients was assessed. ITGA4 q-realtime PCR was performed using Applied Biosystems, TaqMan gene expression assay. ITGA4 gene-specific CpG methylation was investigated in real time using pyrosequencing technology. Results: ITGA4 was differentially expressed in CLL patients. The CpG sites-1, 2 and 3 showed significantly higher mean levels than healthy controls (p = <0.001, 0.007 and 0.009). Significant association between CpG site-1 and CLL has been detected using age-adjusted logistic regression (p < 0.001). Conclusion: Hypermethylation at ITGA4 gene CpG sites (1,2,3) is a characteristic feature in CLL.

Chitosan oligosaccharides packaged into rat adipose mesenchymal stem cells-derived extracellular vesicles facilitating cartilage injury repair and alleviating osteoarthritis

“…However, few reports have shown the roles of ITGA4 , BAD , PPP2R3A , CPT1B , ACACB , CACNA1S , CACNG1 , and MAPK12 in cartilage injury repair. ITGA4 encodes a member of the integrin α chain family, and its high level is associated with many diseases, such as chronic lymphocytic leukemia [ 55 ], gastrointestinal stromal tumors [ 56 ] and colorectal cancer [ 57 ]. BAD plays a key role in mitochondria-dependent apoptosis, and its inactivation contributes to the development of rheumatoid arthritis by conferring apoptosis resistance in synovial sub lining macrophages [ 58 ].…”

Interference With ACSL1 Gene in Bovine Adipocytes: Transcriptome Profiling of mRNA and lncRNA Related to Unsaturated Fatty Acid Synthesis

“…In this study, the ECM-receptor interaction was substantially enriched in 3 DEMs, including ITGA3, ITGA4 , and COL4A5 . These three genes were more closely related to diseases ( 64 – 66 ), but studies have found that ITGA3, ITGA4 , and COL4A1 may be involved in the composition of adipocyte extracellular matrix and the differentiation of adipocytes ( 67 ).…”

DNA Methylation of PI3K/AKT Pathway-Related Genes Predicts Outcome in Patients with Pancreatic Cancer: A Comprehensive Bioinformatics-Based Study

“…Opposingly, methylation of the ITGA4 gene that encodes another integrin alpha subunit (α4) is already described as a biomarker for different cancers [ 77 , 78 , 79 , 80 ], but not in PCA. Interestingly, we found that higher methylation levels of CpGs present in the first exon of ITGA4 were associated with lower gene expression and worse outcome.…”