Juniperus communis essential oil limit the biofilm formation of Mycobacterium avium and Mycobacterium intracellulare on polystyrene in a temperature-dependent manner

Peruč, Dolores; Tićac, Brigita; Broznić, Dalibor; Maglica, Željka; Šarolić, Mladenka; Gobin, Ivana

doi:10.1080/09603123.2020.1741519

Cited by 9 publications

(13 citation statements)

References 52 publications

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“…Our study into the effects of temperature on the mycobacterial biofilm formation revealed a temperature of 25 • C to be the most favorable for biofilm formation. At this temperature, J. communis EO showed the weakest anti-adhesion and antibiofilm activity against NTM on polystyrene [33].…”

Section: Discussionmentioning

confidence: 94%

“…Due to the extreme resistance of OPPP biofilms including those of NTM, new approaches or new substances are needed to fight biofilm formation and destruction. Our previous studies have demonstrated the great potential of EOs as inhibitors of mycobacterial adhesion or biofilm formation on polystyrene as well as inhibitors of adhesion to living cells (amoebae and HeLa cells) [27,32,33,43]. The EOs of J. communis and H. italicum from coastal regions of Croatia have been shown to be particularly effective.…”

Section: Discussionmentioning

confidence: 99%

“…To determine the potential interaction effect of J. communis and H. italicum EOs on NTM, the checkerboard synergy method was used, as described previously [33][34][35]. Briefly, stock solutions and serial two-fold dilutions of each EO were prepared in 7H9S.…”

Section: Checkerboard Synergy Methodsmentioning

confidence: 99%

“…American Type Culture Collection (ATCC) strains were used in these experiments: Mycobacterium avium ssp. avium (serotype 2) ATCC 25291, Mycobacterium intracellulare ATCC 13950, and Mycobacterium gordonae ATCC 14470, and were cultured as described previously [27,32,33]. Briefly, bacterial strains were subcultured twice in Middlebrook 7H9 broth (7H9S, Difco, Detroit, Michigan, USA) containing 10% albumin-dextrose-catalase enrichment (ADC, Biolife Italiana, Milano, Italy) and 0.05% Tween 80 (Biolife Italiana, Milano, Italy) at 30 • C (M. gordonae) or 37 • C (M. avium and M. intracellulare) for at least 14 days to obtain 10 8 CFU mL −1 .…”

Section: Strains and Growth Mediamentioning

confidence: 99%

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Biofilm Degradation of Nontuberculous Mycobacteria Formed on Stainless Steel Following Treatment with Immortelle (Helichrysum italicum) and Common Juniper (Juniperus communis) Essential Oils

et al. 2021

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Nontuberculous mycobacteria, like other opportunistic premise plumbing pathogens, produce resistant biofilms on various surfaces in the plumbing system including pipes, tanks, and fittings. Since standard methods of water disinfection are ineffective in eradicating biofilms, research into new agents is necessary. Essential oils (EOs) have great potential as anti-biofilm agents. Therefore, the purpose of this research was to investigate the potential anti-biofilm effect of common juniper (Juniperus communis) and immortelle (Helichrysum italicum) EOs. Minimum inhibitory concentrations (MIC), minimum bactericidal concentrations (MBC), and minimum effective concentrations of EOs on Mycobacterium avium, M. intracellulare, and M. gordonae were tested. Additionally, biofilms on the surface of a stainless steel disc were treated with single or mixed concentration of EOs, in order to investigate their degeneration via the bacterial count and confocal laser scanning microscopy (CLSM). H. italicum EO showed the strongest biofilm degradation ability against all Mycobacteria strains that were tested. The strongest effect in the biofilm degradation after the single or mixed applications of EOs was observed against M. gordonae, followed by M. avium. The most resistant was the M. intracellulare biofilm. Synergistic combinations of J. communis and H. italicum EOs therefore seem to be an effective substance in biofilm degradation for use in small water systems such as baths or hot tubs.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Checkerboard Synergy Methodsmentioning

confidence: 99%

Section: Strains and Growth Mediamentioning

confidence: 99%

See 2 more Smart Citations

Biofilm Degradation of Nontuberculous Mycobacteria Formed on Stainless Steel Following Treatment with Immortelle (Helichrysum italicum) and Common Juniper (Juniperus communis) Essential Oils

et al. 2021

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“…", Zagreb, Croatia. The same batch had already been used in our earlier studies, which show their chemical composition (11,18,19).…”

Section: Essential Oilsmentioning

confidence: 99%

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Peruč

Tićac

Broznić

et al. 2020

Archives of Industrial Hygiene and Toxicology

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Acanthamoeba is an opportunistic protozoon, widespread in the aquatic environment, where it can be in endosymbiosis with over 30 pathogenic bacteria, including nontuberculous mycobacteria (NTM). Protozoa play a crucial role in mycobacterial pathogenesis and serve as a reservoir of infection. Since the first step in bacteria making contact with amoebae is adhesion, we were interested in investigating whether essential oils (EOs) can affect it. To that end we investigated the effects of juniper (Juniperus communis) and immortelle (Helichrysum italicum) EOs against Mycobacterium avium, M. intracellulare, and M. gordonae in tap water and against their adhesion to Acanthamoeba castellanii by combining them in synergistic EO concentrations. M. avium and M. intracellulare adhered to A. castellanii to a greater extent than M. gordonae. The adhesion of all NTMs was prevented by the subinhibitory concentrations of EOs. When comparing the effect of synergistic combinations of EOs and the effect of a single concentration from a combination, a higher percentage of adhesion inhibition in all synergistic combinations observed, except against M. gordonae. Neither oil was cytotoxic to A. castellanii. Our findings suggest that the EOs or their components weaken the contact of environmental NTMs and free-living amoebae and indirectly diminish their pathogenic potential, which could be of value in developing strategies for maintenance of water supply systems.

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Impact of Geographical Distribution on Chemical Composition and Biological Potential of Juniperus communis L. Essential Oil: A Case Study From Western Himalayas

Vashisath,

Kaundal,

Kumar

et al. 2024

Chemistry & Biodiversity

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The current study was focused to assess the impact of geographical distribution (1325‐3200 m, asl) on essential oil yield, chemical composition, and biological activities of Juniperus communis leaves. Soil mineral content was also analyzed to understand their impact on chemical composition. EO yield variation were observed (0.22‐0.48%), with highest in JCTH (0.48%) sample. Further, refractive index, specific gravity, optical rotation of EOs revealed notable differences among the samples. Six trace elements in soils were found, with iron being the most abundant at all locations. Total phenolic and flavonoid content indicated significant variations, highest TPC in JCTH (6.100±0.649 mgGAE/g) sample while TFC in JCM (1.126±0.004 mgRE/g) sample. GC/GCMS based metabolite profiling had shown overall thirty metabolites, including terpenes and monoterpene‐hydrocarbons. Free radical scavenging assays showed strong antioxidant activity of JCCI and JCTH samples (IC50‐ABTS=4.222±0.166μg/mL and IC50‐DPPH=3.211±0.307μg/mL, respectively). Moreover, EOs significantly inhibit α‐glucosidase, & α‐amylase enzymes, gram positive and negative bacterial strains. These finding suggested its promising biological potential. Furthermore, identified metabolites based statistical analysis (PCA & HCA) highlighted similarities and discriminations of geographical impacted samples. This research will be helpful to understand the metabolite composition and their accumulation under different environmental conditions.

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Juniperus communis essential oil limit the biofilm formation of Mycobacterium avium and Mycobacterium intracellulare on polystyrene in a temperature-dependent manner

Cited by 9 publications

References 52 publications

Biofilm Degradation of Nontuberculous Mycobacteria Formed on Stainless Steel Following Treatment with Immortelle (Helichrysum italicum) and Common Juniper (Juniperus communis) Essential Oils

Biofilm Degradation of Nontuberculous Mycobacteria Formed on Stainless Steel Following Treatment with Immortelle (Helichrysum italicum) and Common Juniper (Juniperus communis) Essential Oils

Juniper and immortelle essential oils synergistically inhibit adhesion of nontuberculous mycobacteria to Acanthamoeba castellanii

Impact of Geographical Distribution on Chemical Composition and Biological Potential of Juniperus communis L. Essential Oil: A Case Study From Western Himalayas

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