<i>Kodamaea (Pichia) ohmeri</i>fungemia in a pediatric patient admitted in a public hospital

Barros, Jadson Duque De; Nascimento, Suerda Maria Nogueira Do; Araújo, Fernanda Janaína Silva De; Braz, Regina de Fátima dos Santos; Andrade, Vânia Sousa; Theelen, Bart; Boekhout, Teun; Illnait-Zaragozí, María Teresa; Gouveia, Maria Narriman Guimarães; Fernandes, Maria C.; Monteiro, Maria Goretti Lins; Oliveira, Maria Tereza Barreto de

doi:10.3109/13693780902980467

Cited by 26 publications

(12 citation statements)

References 26 publications

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“…Since then, this species has been considered a true clinical pathogen, especially in patients with underlying immunosuppression in the case reports mostly conducted in Asia (e.g., Korea, Turkey, and India) [34]. There are also reports from North America and Brazil in this regard [35, 36].…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and antifungal susceptibility pattern of Candida species isolated from HIV infected Patients with candisiasis

Paul¹,

Kannan²

2019

CMM

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Background and Purpose: Opportunistic fungal infections have been on a growingtrend since the last two decades. Among the opportunistic fungal agents, Candidaspecies, Cryptococcus neoformans, and Aspergillus fumigatus account for most of thelife-threatening infections in immunocompromised individuals. Regarding this, thepresent study aimed to investigate the molecular identification and antifungalsusceptibility pattern of Candida species isolated from HIV-infected patients.Materials and Methods: This study was conducted on 80 clinical samples collectedfrom HIV-infected patients with suspected candidiasis referring to Tagore MedicalCollege and Hospital, Rathinamangalam and Government Hospital of ThoracicMedicine, in Chennai, India, for 18 months (i.e., May 2016-December 2017). Phenotypicand molecular identification was accomplished using internal transcribed spacer region 1(ITS1) and ITS4 primers. The antifungal susceptibility pattern of the isolates against fourantifungal agents was also determined by both disk diffusion and broth dilution methods.Results: In the present study, the prevalence of candidiasis was obtained as 75% (n=60).Candida tropicalis was the predominant identified species. All the emerging species(i.e., Kodamaea ohmeri, Hanseniaspora opuntiae, and C. orthopsilosis) were identifiedthrough molecular identification since the phenotypic identification was inconclusive. Interms of the susceptibility pattern, 63.3% and 18.3% of the isolates were resistant tofluconazole and voriconazole, respectively. Candida albicans was also found to beresistant to amphotericin B.Conclusion: Molecular assay led to the identification of K. ohmeri, H. opuntiae, and C.orthopsilosis, which were multidrug-resistant. This study highlighted the need for theprompt and timely identification of clinical yeast isolates given the emergence of manyrare species and their capability of causing life-threatening infections and outbreaks. Inthe laboratories where molecular diagnostic methods are not available, alternativeservices of reference laboratories can be utilized as cost-effective measures. With regardto the growing prevalence of antifungal drug resistance, antifungal susceptibility testingshould be made mandatory for effective patient management.

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Section: Discussionmentioning

confidence: 99%

Molecular identification and antifungal susceptibility pattern of Candida species isolated from HIV infected Patients with candisiasis

Paul¹,

Kannan²

2019

CMM

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“…There are a few from North America [5], some from Brazil [6] and none from Colombia. The largest series reported in the same site included 38 patients from a tertiary care hospital in North India, with 78.9% of these cases corresponding to neonates in intensive care units.…”

Section: Discussionmentioning

confidence: 99%

Fungemia due to Kodamaea ohmeri in a young infant and review of the literature

Vivas

Beltrán

Múnera

et al. 2016

Medical Mycology Case Reports

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“…The genus Kodamaea currently comprises five species: K. anthrophila , K. kakaduensis , K. laetipori , K. nitidulidarum , and K. ohmeri [1] .When the first case of the fungus was isolated from a pleural effusion, it was initially treated as a contaminant [2] . K. ohmeri is reported to cause higher mortality (50%) in paediatric populations compared with patients with C. tropicalis fungaemia (24%) or non-fungaemia (18.2%) [4] . MALDI-TOF MS is a new proteomic approach that allows for rapid and accurate identification of rare fungi, as well as bacteria [5] .…”

Section: Discussionmentioning

confidence: 99%

Catheter-related bloodstream infection caused by Kodamaea ohmeri in China

Hou

2019

Infection Prevention in Practice

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Summary Kodamaea ohmeri is a rare yeast pathogen that has recently emerged as an important cause of fungaemia in immunocompromised patients. In most cases, identification to the species level requires the adoption of new tools, including matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) or DNA sequencing. As K. ohmeri is a teleomorph of Candida guilliermondii var. membranaefaciens and its susceptibility to fluconazole is variable, a rapid and accurate identification of this yeast is important. Echinocandins may be the first choice for empiric therapy for this pathogen. We report the case of a 25-year-old male patient from North China who developed a catheter-related bloodstream infection caused by K. ohmeri. He was treated with caspofungin in hospital. He improved after removal of the central venous catheter and use of caspofungin as therapy. The K. ohmeri strain was identified using a MALDI-TOF mass spectrometer and a Vitek 2YST card. Definitive identification was obtained by a sequencing test of the internal transcribed spacer regions of the rDNA. Our patient findings, the first reported in mainland China, highlighted the diagnostic challenges associated with catheter-related bloodstream infection caused by fungi.

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Kodamaea (Pichia) ohmerifungemia in a pediatric patient admitted in a public hospital

Cited by 26 publications

References 26 publications

Molecular identification and antifungal susceptibility pattern of Candida species isolated from HIV infected Patients with candisiasis

Molecular identification and antifungal susceptibility pattern of Candida species isolated from HIV infected Patients with candisiasis

Fungemia due to Kodamaea ohmeri in a young infant and review of the literature

Catheter-related bloodstream infection caused by Kodamaea ohmeri in China

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