Lactiplantibacillus plantarum BW2013 protects mucosal integrity and modulates gut microbiota of mice with colitis

Abstract

This study explored the effects of L. plantarum BW2013 on mucosal integrity and gut microbiota of mice with dextran sulphate sodium (DSS) induced colitis. The results show that the clinical symptoms in DSS modelled ulcerative colitis (UC) were improved by L. plantarum BW2013 via decreasing disease activity index (DAI) scores and suppressing inflammatory cell infiltration. Furthermore, L. plantarum BW2013 decreased the levels of diamine oxidase (DAO) activity, myeloperoxidase (MPO) and D-lactic acid (DLA). The mRNA expression of ZO-1, occludin and claudin-1 was upregulated by L. plantarum BW2013, which also increased IL-10 and reduced TNF-α, IL-1β and IL-6 in the colon. 16S rDNA sequencing showed that L. plantarum BW2013 enhanced α-diversity. L.plantarum BW2013 upregulated significantly the abundance of unidentfied_Lachnospiraceae, Lactococcus, Rikenella, Lactobacillus and Odoribacter, which had an inhibitory effect on inflammation and a protective effect on the integrity of the mucosa. These results demonstrate that L. plantarum BW2013 alleviates DSS modelled UC by protecting mucosal integrity and ameliorating the composition of gut microbiota.

Sciadonic acid ameliorates cyclophosphamide‐induced immunosuppression by modulating the immune response and altering the gut microbiota

“…Moreover, Odoribacter and Lachnospiraceae_NK4A136_group reportedly promote the production of butyric acid, which plays an important role in maintaining the internal pH of the gut and protecting intestinal health 45,46 . Rikenella , a beneficial bacterium, has an inhibitory effect on inflammation 47 . Clinical studies have found a higher abundance of Ruminococcus in the gut of patients with inflammatory bowel diseases compared to healthy populations 48 .…”

“…45,46 Rikenella, a beneficial bacterium, has an inhibitory effect on inflammation. 47 Clinical studies have found a higher abundance of Ruminococcus in the gut of patients with inflammatory bowel diseases compared to healthy populations. 48 Furthermore, Desulfovibrio is a pathogenic bacterium that can cause bacteremia.…”

Clostridium butyricum isolated from giant panda can attenuate dextran sodium sulfate-induced colitis in mice

“…However, disadvantages of this treatment include high recurrence rates and low remission rates (Chen et al, 2022). Previous studies showed that probiotic intervention can effectively regulate intestinal inflammation and reduce the damage (Niu et al, 2023;Sharma et al, 2023). Therefore, probiotics have become an alternative for preventing and treating several diseases, such as enteritis.…”

“…Therefore, probiotics have become an alternative for preventing and treating several diseases, such as enteritis. In addition, the DSS-induced mouse enteritis model is easy to operate and has been applied to many probiotics in evaluating the effects on colitis such as Lactobacillus plantarum, Bifidobacterium and so on (Niu et al, 2023;Wang et al, 2023). Consequently, a DSS-induced mouse enteritis model was used to study the effect of C. butyricum isolated from giant panda feces.…”

Preventive therapeutic effect of Lactobacillus‐fermented black wolfberry juice on sodium dextran sulfate‐induced ulcerative colitis in mice

“…(Di Cagno et al., 2019). LP BW2013 alleviated dextran sodium sulfate (DSS)‐modeled UC by protecting mucosal integrity and ameliorating the composition of gut microbiota (Niu et al., 2023). LB treatment attenuated the clinical signs of intestinal inflammation, reducing the intestinal and tumor levels of IL‐6, TNF‐α, IL‐17, IL‐23, and IL‐1 beta (Calheiros Silveira et al., 2020).…”