<i>Lactobacillus acidophilus</i> Alleviated <i>Salmonella</i>‐Induced Goblet Cells Loss and Colitis by Notch Pathway

Wu, Haiqin; Ye, Lulu; Xiao, Lu; Xie, Shuang; Yang, Qian; Yu, Qinghua

doi:10.1002/mnfr.201800552

Cited by 44 publications

(41 citation statements)

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“…Additionally, it has been reported that probiotic bacteria can increase the production of short-chain fatty acids, which have been associated with proliferation of intestinal epithelial cells [ 41 ]. The outcomes of the present research are in line with previous studies showing the positive effects of Lactobacillus supplementation on mucosal architecture in pathogen-exposed birds [ 42 , 43 ], although these studies have also made emphasis on goblet cell hyperplasia and mucin production, which contribute as well for maintaining the integrity of the intestinal barrier. Arguably, our data revealed the importance of L. fermentum for preserving the intestinal ecosystem and, most importantly, for preventing epithelial damage induced by C. jejuni .…”

Section: Discussionsupporting

confidence: 89%

The Probiotic Lactobacillus fermentum Biocenol CCM 7514 Moderates Campylobacter jejuni-Induced Body Weight Impairment by Improving Gut Morphometry and Regulating Cecal Cytokine Abundance in Broiler Chickens

Šefcová

Larrea-Álvarez

et al. 2021

Animals

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This research was conducted to investigate if the administration of the probiotic Lactobacillus fermentum could influence body weight, intestinal morphometry and the cecal cytokine response in Campylobacter jejuni-infected chickens. Seventy-two 1-day old COBB 500 male chicks were allocated randomly into four experimental groups. (I) Control group (C), in which chicks were left untreated. (II) LB group, treated with L. fermentum. (III) Cj group, infected with C. jejuni and (IV) coexposure group in which both bacteria were administered. Body weight was registered and then all birds were slaughtered; samples from the small intestine and caecum were collected at 4- and 7-days post infection. The experiment lasted eleven days. Villi height and crypt depth ratios of the duodenum, jejunum and ileum were evaluated using appropriate software, while reverse transcription quantitative PCR (RT-qPCR) was utilized for assessing transcript levels of key cecal inflammatory cytokines (IL-1β, IL-18, IL-17, IL-15, IL13 and IL-4). Campylobacter-infected birds showed lower body weight values than those supplemented with the probiotic; these birds, in turn, proved to be heavier than those reared under control conditions. L. fermentum administration improved morphometrical parameters of the duodenum, jejunum and ileum; in general, villi were larger and crypts deeper than those identified in control conditions. Moreover, the negative effects elicited by C. jejuni were not observed in chickens exposed to the probiotic. Significant differences were also determined with regards to transcript abundance of all evaluated cytokines in the caecum. C. jejuni induced a downregulation of the studied interleukins; however, such a response was heightened by administration of L. fermentum, with an increase rate of transcription that promoted a more effective response to a C. jejuni infection. The effects of experimental treatments proved to vary between sampling points. Conclusively, these results demonstrate that L. fermentum lessens the negative effects elicited by C. jejuni on body weight by alleviating the impact on intestinal morphometry and cecal cytokine response, which ultimately improve chicken growth performance.

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Section: Discussionsupporting

confidence: 89%

The Probiotic Lactobacillus fermentum Biocenol CCM 7514 Moderates Campylobacter jejuni-Induced Body Weight Impairment by Improving Gut Morphometry and Regulating Cecal Cytokine Abundance in Broiler Chickens

Šefcová

Larrea-Álvarez

et al. 2021

Animals

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“…Previous studies have shown that oral administration of Lactobacillus acidophilus obviously alleviated S . Typhimurium-induced goblet cells loss in mice [12]. A select mixture of Bacillus licheniformis and Bacillus subtilis ameliorated E. coli -induced decreases in the number of ileal GCs via increasing Atoh1 mRNA expression in newly weaned pigs [10].…”

Section: Introductionmentioning

confidence: 99%

Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis

Liu

Xia

et al. 2019

Microorganisms

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Salmonella is important as both a cause of clinical disease in swine and as a source of food-borne transmission of disease to humans. Lactobacillus and Bacillus are often used as antibiotic substitutes to prevent Salmonella infection. In this study, we evaluated the effects of a select mixture of Lactobacillus johnsonii L531, Bacillus licheniformis BL1721 and Bacillus subtilis BS1715 (LBB-mix) in prevention of Salmonella enterica serovar Infantis infection in a pig model. LBB-mix was orally administered to newly weaned piglets for seven days before S. Infantis challenge. LBB-mix pretreatment ameliorated S. Infantis-induced fever, leukocytosis, growth performance loss, and ileal inflammation. Pre-administration of LBB-mix reduced the number of Salmonella in the feces but increased the number of goblet cells in the ileum. S. Infantis infection resulted in an increase in cell death in the ileum, this increase was attenuated by LBB-mix consumption. Claudin 1 and cleaved caspase-1 expression was decreased in the ileum of pigs challenged with S. Infantis, but not in pigs pretreated with LBB-mix. In conclusion, our data indicate that a select LBB-mix has positive effects on controlling S. Infantis infection via alleviating inflammation and maintaining the intestinal mucosal barrier integrity in pigs.

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“…During infection, parasites seem to promote GC differentiation and mucin production, likely via type 2 cytokines such as IL-4, IL-9, and IL-13 (11)(12)(13)(14)(15)(16)(17)(18). In contrast, under bacterial infection, such as infection with Citrobacter, Salmonella, and Listeria, significant reductions in GC number and mucin production were reported (19)(20)(21). It is not clear whether these are the cause of fullblown infection or the consequence of infection-induced pathological injury.…”

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confidence: 99%

Type 3 Innate Lymphoid Cells Direct Goblet Cell Differentiation via the LT–LTβR Pathway duringListeriaInfection

Pian

Chai

Ren

et al. 2020

The Journal of Immunology

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M.Z. and Q.C. designed the study and wrote the manuscript. Q.C. and Y.P. performed the experiments and analyzed the data. B.R. analyzed the RNA-seq data. Y.W. and M.L. performed the experiments. J.Q. provided reagents and discussed the data.The sequences presented in this article have been submitted to the Gene Expression Omnibus (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE150071) under accession number GSE150071.

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Lactobacillus acidophilus Alleviated Salmonella‐Induced Goblet Cells Loss and Colitis by Notch Pathway

Abstract: The study demonstrates that colitis improvement is controlled by Lactobacillus ATCC 4356 by regulation of the Notch pathway; this finding will be useful for prevention against animal S. typhimurium infection.

Cited by 44 publications

References 50 publications

The Probiotic Lactobacillus fermentum Biocenol CCM 7514 Moderates Campylobacter jejuni-Induced Body Weight Impairment by Improving Gut Morphometry and Regulating Cecal Cytokine Abundance in Broiler Chickens

The Probiotic Lactobacillus fermentum Biocenol CCM 7514 Moderates Campylobacter jejuni-Induced Body Weight Impairment by Improving Gut Morphometry and Regulating Cecal Cytokine Abundance in Broiler Chickens

Oral Administration of a Select Mixture of Lactobacillus and Bacillus Alleviates Inflammation and Maintains Mucosal Barrier Integrity in the Ileum of Pigs Challenged with Salmonella Infantis

Type 3 Innate Lymphoid Cells Direct Goblet Cell Differentiation via the LT–LTβR Pathway duringListeriaInfection

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