Lactobacillus bulgaricus and Lactobacillus plantarum improve diabetic wound healing through modulating inflammatory factors

Abstract

Probiotics are nonpathogenic bacterial strains that exert beneficial effects on the host. Previous studies have shown that topical use of some strains of probiotic bacteria have good effects on the healing of cutaneous wounds. In the current study, the wound healing potentials of bacterial probiotics on diabetic cutaneous wounds were evaluated. The effects of probiotics on migration, the viability of fibroblasts, and macrophage proliferation were measured through using wound healing assay, methylthiazol tetrazolium assay, and bromodeoxyuridine, respectively. In this regard, in vivo diabetic wound healing experiments in Wistar rats following treatment with nontoxic concentrations of Lactobacillus bulgaricus and Lactobacillus plantarum were conducted. The histopathological and gene expression analyses were performed following removal of wound sites 3, 7, and 14 days postwounding. Results showed that treatment with probiotics accelerated the healing process of diabetic wounds and modulated the inflammatory cells in wound sites during a 14-day period postwounding. The altered mRNA levels of inflammatory cytokines were observed in wound sites following treatment with probiotics. The findings of the current study reveal that L. bulgaricus and L. plantarum could improve the healing of diabetic wounds via regulation of inflammation.

Insight Into the Beneficial Role of Lactiplantibacillus plantarum Supernatant Against Bacterial Infections, Oxidative Stress, and Wound Healing in A549 Cells and BALB/c Mice

“…Lactobacillus bulgaricus and Lpb. plantarum accelerated wound healing by decreasing IL1 β and TNF α , and upregulating IL-10 expression in diabetic Wistar rats ( Mohtashami et al, 2020 ). The probiotic strains have been consistently reported to modulate the pro-inflammatory cytokine, IL-6, and upregulate the level of anti-inflammatory cytokine, IL-10 ( Karamese et al, 2016 ; Holowacz et al, 2018 ; Johnson et al, 2020 ).…”

Gestational Diabetes, Colorectal Cancer, Bariatric Surgery, and Weight Loss among Diabetes Mellitus Patients: A Mini Review of the Interplay of Multispecies Probiotics

“…bulgaricus . In addition, Mohtashami et al [ 68 ] evaluated the wound-healing potentials of probiotics on diabetic cutaneous wounds after surgery in rats ( n = 27). Lactobacillus delbrueckii subsp.…”

“…Lactobacillus delbrueckii subsp. bulgaricus and Lactiplantibacillus plantarum probiotics were administered topically [ 68 ]. Probiotics have demonstrated some benefits at the skin level through oral and topical administration [ 68 ].…”

Avoidance of milk and dairy products after oral surgery—is such a recommendation still valid? A cross-sectional study among German and international oral and maxillofacial surgeons and dental practitioners with review of the literature

“…Enhanced wound healing, increased collagen production Khodaii, 2019 [20] Animal model study L. reuteri Enhanced collagen production, accelerated wound healing Han, 2019 [21] Animal model study L. reuteri Promoted wound healing through activation of GMSCs Han, 2020 [26] Animal model study L. reuteri Inhibited inflammation and enhanced wound healing through promoting MSCs Mohtashami, 2020 [23] Animal model study L. bulgaricus, L. plantarum Enhanced wound healing Ong, 2020 [24] Animal model study L. plantarum Suppressed Staphylococcus aureus, and thereby improved wound healing Moreira, 2021 [25] Animal model study L. johnsonii, L. paracasei, and L. rhamnosus Promoted skin wound closure and decreased scar formation and inflammation healing to milk and dairy products [6]. Compared to the international collective, there was a striking difference regarding the sources of information given by OMFS and specialist dentists for oral surgery (Table 8).…”