<i>Lactobacillus crispatus</i> accelerates re‐epithelialization in vaginal epithelial cell line <scp>MS</scp>74

Takada, Kazuko; Komine‐Aizawa, Shihoko; Kuramochi, Tomoya; Ito, Shun; Trinh, Quang Duy; Pham, Ngan Thi Kim; Sasano, Mari; Hayakawa, Satoshi

doi:10.1111/aji.13027

Cited by 35 publications

(22 citation statements)

References 61 publications

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“…The results reported here are consistent with how L. casei and B. breve affect gastrointestinal cell proliferation but differ from the reported antimigration effect of G. vaginalis on HeLa cells (51) and promigration effect of L. crispatus on MS374 cells (52). The cancerous cervical origin of both HeLa and MS374 cell lines likely compromises these cells as models to study migration or proliferation.…”

Section: Discussionsupporting

confidence: 75%

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The Cervicovaginal Microbiota-Host Interaction Modulates Chlamydia trachomatis Infection

Edwards

Smith

McComb

et al. 2019

mBio

128

144

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The mechanism(s) by which Lactobacillus-dominated cervicovaginal microbiota provide a barrier to Chlamydia trachomatis infection remain(s) unknown. Here we evaluate the impact of different Lactobacillus spp. identified via culture-independent metataxonomic analysis of C. trachomatis-infected women on C. trachomatis infection in a three-dimensional (3D) cervical epithelium model. Lactobacillus spp. that specifically produce d(−) lactic acid were associated with long-term protection against C. trachomatis infection, consistent with reduced protection associated with Lactobacillus iners, which does not produce this isoform, and with decreased epithelial cell proliferation, consistent with the observed prolonged protective effect. Transcriptomic analysis revealed that epigenetic modifications involving histone deacetylase-controlled pathways are integral to the cross talk between host and microbiota. These results highlight a fundamental mechanism whereby the cervicovaginal microbiota modulates host functions to protect against C. trachomatis infection. IMPORTANCE The vaginal microbiota is believed to protect women against Chlamydia trachomatis, the etiologic agent of the most prevalent sexually transmitted infection (STI) in developed countries. The mechanism underlying this protection has remained elusive. Here, we reveal the comprehensive strategy by which the cervicovaginal microbiota modulates host functions to protect against chlamydial infection, thereby providing a novel conceptual mechanistic understanding. Major implications of this work are that (i) the impact of the vaginal microbiota on the epithelium should be considered in future studies of chlamydial infection and other STIs and (ii) a fundamental understanding of the cervicovaginal microbiota’s role in protection against STIs may enable the development of novel microbiome-based therapeutic strategies to protect women from infection and improve vaginal and cervical health.

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Section: Discussionsupporting

confidence: 75%

“…Conversely, migration of MS374 cells was increased upon exposure to L. crispatus culture supernatants relative to control cell culture medium (52). In another study, lactate and acetate produced by Lactobacillus casei and Bifidobacterium breve inhibited gut epithelial cell proliferation (53).…”

Section: Discussionmentioning

confidence: 99%

The Cervicovaginal Microbiota-Host Interaction Modulates Chlamydia trachomatis Infection

Edwards

Smith

McComb

et al. 2019

mBio

128

144

View full text Add to dashboard Cite

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“…L. crispatus and L. gasseri are commensal bacteria found in both human GI tract (Walter, 2008) and lower female urogenital tract. Their implication on women's health have been explored in the recent years (Stapleton et al, 2011;Matsuda et al, 2018;Phukan et al, 2018;Takada et al, 2018). Preliminary clinical trials have shown that intro-vaginal probiotic treatment with L. crispatus (intravaginal suppository probiotic Lactin-V; Osel) could significantly lower the reoccurrence rate of urinary tract infections in premenopausal women (Stapleton et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Preliminary clinical trials have shown that intro-vaginal probiotic treatment with L. crispatus (intravaginal suppository probiotic Lactin-V; Osel) could significantly lower the reoccurrence rate of urinary tract infections in premenopausal women (Stapleton et al, 2011). L. crispatus JCM 7696 has also been shown to accelerate wound healing in vaginal epithelial cell line (Takada et al, 2018). L. gasseri American Type Culture Collection (ATCC) 9857 has been found to significantly inhibit the adhesion of Trichomonas vaginalis to human vaginal ectocervical cells (Phukan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts

et al. 2020

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Lactobacillus crispatus and Lactobacillus gasseri are two of the main Lactobacillus species found in the healthy vaginal microbiome and have also previously been identified and isolated from the human gastrointestinal (GI) tract. These two ecological niches are fundamentally different, notably with regards to the epithelial cell type, nutrient availability, environmental conditions, pH, and microbiome composition. Given the dramatic differences between these two environments, we characterized strains within the same Lactobacillus species isolated from either the vaginal or intestinal tract to assess whether they are phenotypically and genetically different. We compared the genomes of the Lactobacillus strains selected in this study for genetic features of interest, and performed a series of comparative phenotypic assays including small intestinal juice and acid resistance, carbohydrate fermentation profiles, lactic acid production, and host interaction with intestinal Caco-2 and vaginal VK2 cell lines. We also developed a simulated vaginal fluid (SVF) to study bacterial growth in a proxy vaginal environment and conducted differential transcriptomic analysis between SVF and standard laboratory MRS medium. Overall, our results show that although strain-specific variation is observed, some phenotypic differences seem associated with the isolation source. We encourage future probiotic formulation to include isolation source and take into consideration genetic and phenotypic features for use at various body sites.

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“…However, it was reported recently that L . crispatus promotes repair of injured vaginal epithelial cells by promoting the secretion of vascular endothelial growth factor A (VEGF), which may help to reduce subsequent acquisition of urogenital tract infections [44]. L .…”

Section: Discussionmentioning

confidence: 99%

Diversity of endocervical microbiota associated with genital Chlamydia trachomatis infection and infertility among women visiting obstetrics and gynecology clinics in Malaysia

et al. 2019

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The cervical microbiota constitutes an important protective barrier against the invasion of pathogenic microorganisms. A disruption of microbiota within the cervical milieu has been suggested to be a driving factor of sexually transmitted infections. These include Chlamydia trachomatis which frequently causes serious reproductive sequelae such as infertility in women. In this study, we profiled the cervical microbial composition of a population of 70 reproductive-age Malaysian women; among which 40 (57.1%) were diagnosed with genital C. trachomatis infection, and 30 (42.8%) without C. trachomatis infection. Our findings showed a distinct compositional difference between the cervical microbiota of C. trachomatis-infected subjects and subjects without C. trachomatis infection. Specifically, significant elevations of mostly strict and facultative anaerobes such as Streptococcus, Megasphaera, Prevotella, and Veillonella in the cervical microbiota of C. trachomatis-positive women were detected. The results from the current study highlights an interaction of C. trachomatis with the environmental microbiome in the endocervical region.

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Lactobacillus crispatus accelerates re‐epithelialization in vaginal epithelial cell line MS74

Cited by 35 publications

References 61 publications

The Cervicovaginal Microbiota-Host Interaction Modulates Chlamydia trachomatis Infection

The Cervicovaginal Microbiota-Host Interaction Modulates Chlamydia trachomatis Infection

Comparative Analysis of Lactobacillus gasseri and Lactobacillus crispatus Isolated From Human Urogenital and Gastrointestinal Tracts

Diversity of endocervical microbiota associated with genital Chlamydia trachomatis infection and infertility among women visiting obstetrics and gynecology clinics in Malaysia

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