<i>Lactobacillus fermentum</i>RC‐14 Inhibits<i>Staphylococcus aureus</i>Infection of Surgical Implants in Rats

Gan, Bing Siang; Kim, Juan; Reid, Gregor; Cadieux, Peter A.; Howard, Jeffrey C.

doi:10.1086/340126

Cited by 144 publications

(95 citation statements)

References 15 publications

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“…Our results raise intriguing questions about the usefulness of probiotics, including LGG, in the context of infection. Indeed, Gan et al (45) reported that Lactobacillus fermentum RC-14 and its secreted biosurfactant inhibit S. aureus infection and bacterial adherence to surgical implants.…”

Section: Discussionmentioning

confidence: 99%

Probiotic Lactobacillus rhamnosus Inhibits the Formation of Neutrophil Extracellular Traps

Vong

Lorentz

Assa

et al. 2014

The Journal of Immunology

107

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Alterations in the gut microbiota, referred to as dysbiosis, are strongly associated with chronic inflammatory bowel diseases. Neutrophils are one of the earliest cells to respond to an inflammatory stimulus and, in the inflamed intestine, constitute a significant proportion of cells that are recruited. Whereas probiotics have been shown to exert beneficial effects on gut homeostasis, their effect on neutrophil extracellular trap (NET) formation remains undefined. Therefore, the aim of this study was to determine the effects of the probiotic Lactobacillus rhamnosus GG (LGG) on the dynamics of NET formation. Bone marrow‐derived neutrophils (BMDN) were isolated from C57BL6 mice and co‐incubated with LGG (1 h, 37°C, 5% CO2) prior to stimulation with phorbol 12‐myristate 13‐acetate (PMA, 100 nM), which is an inducer of NET formation. Measurements of extracellular DNA (Sytox Green, 5 nM) and immunofluorescence microscopy staining for DNA, elastase and histones demonstrated that LGG mediated a concentration‐dependent reduction in NET‐osis. Given the proximity of recruited neutrophils to the lumen of the inflamed gut and the resident intestinal microbiota, it is important to define how neutrophil‐microbe interactions affect the local microenvironment. This work was funded by CIHR (operating grant MOP‐89894).

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Section: Discussionmentioning

confidence: 99%

Probiotic Lactobacillus rhamnosus Inhibits the Formation of Neutrophil Extracellular Traps

Vong

Lorentz

Assa

et al. 2014

The Journal of Immunology

107

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“…In recent years, other versions of microbial antagonisms, which do not directly kill pathogens, have also been investigated. One interesting example is that Lactobacillus fermentum RC-14, a probiotic bacterial isolate, inhibited acute Staphylococcus aureus infection (19). The probiotic bacteria did not appear to affect pathogen growth: rather, the pathogen secretes cell surface extracellular matrix-binding proteins and biosurfactant that somehow prevented pathogen adherence to surgical implants and inhibited S. aureus infection.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal Bacillus thuringiensis Silences Erwinia carotovora Virulence by a New Form of Microbial Antagonism, Signal Interference

Dong

Zhang

et al. 2004

Appl Environ Microbiol

228

112

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It is commonly known that bacteria may produce antibiotics to interfere with the normal biological functions of their competitors in order to gain competitive advantages. Here we report that Bacillus thuringiensis suppressed the quorum-sensing-dependent virulence of plant pathogen Erwinia carotovora through a new form of microbial antagonism, signal interference. E. carotovora produces and responds to acyl-homoserine lactone (AHL) quorum-sensing signals to regulate antibiotic production and expression of virulence genes, whereas B. thuringiensis strains possess AHL-lactonase, which is a potent AHL-degrading enzyme. B. thuringiensis did not seem to interfere with the normal growth of E. carotovora; rather, it abolished the accumulation of AHL signal when they were cocultured. In planta, B. thuringiensis significantly decreased the incidence of E. carotovora infection and symptom development of potato soft rot caused by the pathogen. The biocontrol efficiency is correlated with the ability of bacterial strains to produce AHL-lactonase. While all the seven AHL-lactonaseproducing B. thuringiensis strains provided significant protection against E. carotovora infection, Bacillus fusiformis and Escherichia coli strains that do not process AHL-degradation enzyme showed little effect in biocontrol. Mutation of aiiA, the gene encoding AHL-lactonase in B. thuringiensis, resulted in a substantial decrease in biocontrol efficacy. These results suggest that signal interference mechanisms existing in natural ecosystems could be explored as a new version of antagonism for prevention of bacterial infections.

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“…Nevertheless, the application of viable lactic acid bacteria to an infected wound would represent a paradigm shift in current surgical practice. In a series of animal studies, L. fermentum RC-14 and proteins produced by this organisms were shown to prevent severe Staphyoloccus aureus surgical implant infection (40). Although this does not prove human efficacy, the concept illustrates a different approach to wound infection management.…”

Section: Probiotics For Surgical Infectionsmentioning

confidence: 99%

Potential Uses of Probiotics in Clinical Practice

Reid

Jaß

Sebulsky³

et al. 2003

Clin Microbiol Rev

Self Cite

747

450

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Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host. There is now mounting evidence that selected probiotic strains can provide health benefits to their human hosts. Numerous clinical trials show that certain strains can improve the outcome of intestinal infections by reducing the duration of diarrhea. Further investigations have shown benefits in reducing the recurrence of urogenital infections in women, while promising studies in cancer and allergies require research into the mechanisms of activity for particular strains and better-designed trials. At present, only a small percentage of physicians either know of probiotics or understand their potential applicability to patient care. Thus, probiotics are not yet part of the clinical arsenal for prevention and treatment of disease or maintenance of health. The establishment of accepted standards and guidelines, proposed by the Food and Agriculture Organization of the United Nations and the World Health Organization, represents a key step in ensuring that reliable products with suitable, informative health claims become available. Based upon the evidence to date, future advances with single- and multiple-strain therapies are on the horizon for the management of a number of debilitating and even fatal conditions

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Lactobacillus fermentumRC‐14 InhibitsStaphylococcus aureusInfection of Surgical Implants in Rats

Cited by 144 publications

References 15 publications

Probiotic Lactobacillus rhamnosus Inhibits the Formation of Neutrophil Extracellular Traps

Probiotic Lactobacillus rhamnosus Inhibits the Formation of Neutrophil Extracellular Traps

Insecticidal Bacillus thuringiensis Silences Erwinia carotovora Virulence by a New Form of Microbial Antagonism, Signal Interference

Potential Uses of Probiotics in Clinical Practice

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