Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds

Nakano, Akiyo; Shimazu, Mitsuaki; Toh, Y.; Nakajima, Fumihiko; Nagayama, Masahiro; Hisamatsu, Shin; Kato, Yukio; Takagi, Misako; Takami, Hideto; Akita, Hiroaki; Matsumoto, M.; Masaoka, Toshio; Murakami, Masaru

doi:10.1111/j.1740-0929.2009.00633.x

Cited by 28 publications

(31 citation statements)

References 20 publications

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“…We found one group of reads (the most dominant read type in the antral and glandular samples from horse 2) to be a sister group of L. jensenii and L. fornicalis, which may represent a taxonomically uncharacterized species. In addition to this group, we found reads that clustered with the type strains of L. hayakitensis and L. equigenerosi, two distinct Lactobacillus species that were reported to be the predominant lactobacilli in the intestinal microbiota of healthy thoroughbred horses (41). The observation that these species are also abundant in the gastric mucosae of healthy horses suggests that these species may also form a predominant component of the local microbiota of the gastric mucosa.…”

Section: Discussionmentioning

confidence: 76%

Equine Stomachs Harbor an Abundant and Diverse Mucosal Microbiota

Perkins

Bakker

Burton

et al. 2012

Appl Environ Microbiol

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ABSTRACTLittle is known about the gastric mucosal microbiota in healthy horses, and its role in gastric disease has not been critically examined. The present study used a combination of 16S rRNA bacterial tag-encoded pyrosequencing (bTEFAP) and fluorescencein situhybridization (FISH) to characterize the composition and spatial distribution of selected gastric mucosal microbiota of healthy horses. Biopsy specimens of the squamous, glandular, antral, and any ulcerated mucosa were obtained from 6 healthy horses by gastroscopy and from 3 horses immediately postmortem. Pyrosequencing was performed on biopsy specimens from 6 of the horses and yielded 53,920 reads in total, with 631 to 4,345 reads in each region per horse. The microbiome segregated into two distinct clusters comprised of horses that were stabled, fed hay, and sampled at postmortem (cluster 1) and horses that were pastured on grass, fed hay, and biopsied gastroscopically after a 12-h fast (cluster 2). The types of bacteria obtained from different anatomic regions clustered by horse rather than region. The dominant bacteria in cluster 1 wereFirmicutes(>83% reads/sample), mainlyStreptococcusspp.,Lactobacillusspp. and,Sarcinaspp. Cluster 2 was more diverse, with predominantlyProteobacteria,Bacteroidetes, andFirmicutes, consisting ofActinobacillusspp.Moraxellaspp.,Prevotellaspp., andPorphyromonasspp.Helicobactersp. sequences were not identified in any of 53,920 reads. FISH (n= 9) revealed bacteria throughout the stomach in close apposition to the mucosa, with significantly moreStreptococcusspp. present in the glandular region of the stomach. The equine stomach harbors an abundant and diverse mucosal microbiota that varies by individual.

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Section: Discussionmentioning

confidence: 76%

Equine Stomachs Harbor an Abundant and Diverse Mucosal Microbiota

Perkins

Bakker

Burton

et al. 2012

Appl Environ Microbiol

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“…Of these species, L. equigenerosi, L. equi, and L. hayakitensis have not been isolated from other niches and are considered host specific (11,23). Several Clostridium spp.…”

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confidence: 99%

Lactobacillus equigenerosi Strain Le1 Invades Equine Epithelial Cells

Botha

Botes

Loos

et al. 2012

Appl Environ Microbiol

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ABSTRACTLactobacillus equigenerosistrain Le1, a natural inhabitant of the equine gastrointestinal tract, survived pH 3.0 and incubation in the presence of 1.5% (wt/vol) bile salts for at least 2 h. Strain Le1 showed 8% cell surface hydrophobicity, 60% auto-aggregation, and 47% coaggregation withClostridium difficileC6. Only 1% of the cells adhered to viable buccal epithelial cells and invaded the cells within 20 min after contact. Preincubation of strain Le1 in a buffer containing pronase prevented adhesion to viable epithelial cells. Preincubation in a pepsin buffer delayed invasion from 20 min to 1 h. Strain Le1 did not adhere to nonviable epithelial cells. Administration ofL. equigenerosiLe1 (1 × 109CFU per 50 kg body weight) to healthy horses did not increase white blood cell numbers. Differential white blood cell counts and aspartate aminotransferase levels remained constant. Glucose, lactate, cholesterol, and urea levels remained constant during administration withL. equigenerosiLe1 but decreased during the week after administration.

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“…Of the five strains, four have been identified as strains of Lactobacillus mucosae, L. johnsonii, L. equigenerosi and L. buchneri (Morita et al, 2009). Although the fifth isolate, DI70…”

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confidence: 99%

“…S1 in IJSEM Online). L. delbrueckii, L. johnsonii, L. crispatus and Lactobacillus amylovorus, strains of which have been isolated from the contents of the equine gastrointestinal tract by Bailey et al (2003), Al Jassim et al (2005) and Morita et al (2009), belong to the L. delbrueckii phylogenetic group. Although we could not isolate other strains of the same species as DI70…”

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confidence: 99%

Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse

Shimazu

Shiono

Toh

et al. 2010

International Journal of Systematic and Evolutionary Microbiology

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We previously isolated five strains of putative lactobacilli from the faeces of a thoroughbred horse (a 4-year-old male). Of the five strains, four were identified as members of existing Lactobacillus species; however, sequence analysis of the 16S rRNA gene revealed that the fifth isolate, DI70T ,showed approximately 97 % identity (1325/1366 bp) with the type strain of Lactobacillus delbrueckii. Therefore, we considered the possibility that DI70 T represents a novel species of the genus Lactobacillus. Cells of strain DI70 T were Gram-stain-positive, catalase-negative, nonspore-forming, non-motile rods. In phylogenetic trees constructed on the basis of 16S rRNA gene sequences, strain DI70 T formed a subcluster in the L. delbrueckii phylogenetic group and was closely related to L. delbrueckii, Lactobacillus crispatus and Lactobacillus jensenii. However, analysis of DNA-DNA relatedness showed that DI70 T was genetically distinct from its phylogenetic relatives. The isolate also exhibited distinct biochemical and physiological characteristics when compared with its phylogenetic relatives. It required anaerobic conditions for growth on agar medium. The results indicate that isolate DI70

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Lactobacillus hayakitensis, L. equigenerosi and L. equi, predominant lactobacilli in the intestinal flora of healthy thoroughbreds

Cited by 28 publications

References 20 publications

Equine Stomachs Harbor an Abundant and Diverse Mucosal Microbiota

Equine Stomachs Harbor an Abundant and Diverse Mucosal Microbiota

Lactobacillus equigenerosi Strain Le1 Invades Equine Epithelial Cells

Lactobacillus equicursoris sp. nov., isolated from the faeces of a thoroughbred racehorse

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