Lactobacillus alleviated obesity induced by high‐fat diet in mice

Abstract

Obesity is closely related to dyslipidaemia, diabetes and other metabolic syndromes. Long-term consumption of a high-fat diet (HFD) is an important risk factor that can lead to obesity. In the current research, three Lactobacillus strains, namely, Loigolactobacillus coryniformis subsp. torquens T3 (T3), Lacticasebacillus paracasei subsp. paracasei M5 (M5), and Lacticaseibacillus paracasei subsp. paracasei X12 (X12), were tested to determine their inhibitory effects on HFD-induced obesity. The results showed that M5, T3, and X12 significantly decreased the body weight gain, Lee's index and adipose index. T3 showed significant effects on reducing serum TG levels to 0.92 mmol/ml and increasing HDL-C levels to 2.18 mmol/ml. The M5 treatment significantly reduced the serum TG level and leptin content to 1.11 mmol/ml and 3.7 ng/ml, respectively, and it increased the HDL-C level and adiponectin content to 2.35 mmol/ml and 7 ng/ml, respectively. M5 and T3 dramatically ameliorated hepatic steatosis in HFD-treated mice by reducing the liver index, lipid droplet number in the liver and TC levels in the liver. Gene expression of PPAR-γ and TNF-α was notably downregulated and FAS was upregulated by T3 and M5 treatment. Additionally, administration of M5 and T3 modified the diversity of the gut microbiota with increased OTU number, ACE index, and Chao1, and decreased the Shannon index and the Bacteroidetes /Firmicutes ratio. Overall, our results indicate that Lactobacillus may be used to prevent obesity and gut dysbiosis.

Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats

“…SCFAs increased the relative abundance of Chlamydia and Lactobacillus and decreased the relative abundance of Bacteroides, Romboutsia, Lactococcus and Alloprevotella. Consistent with previous studies, the increase in Chlamydia is associated with obesity-related metabolic syndrome, and the increase in Lactobacillus can reverse obesity induced by HFD and regulate the microbiological SCFAs hormone pathway to regulate metabolism (43,44). The increase in Bacteroides abundance is related to the level of estradiol (18).…”

Molecular networking-based lipid profiling and multi-omics approaches reveal new contributions of functional vanilloids to gut microbiota and lipometabolism changes

“…2 A). According to previous studies reporting a decrease in the Chao1 index-related diversity associated with HFD intake ( Campbell et al, 2019 , Song et al, 2021 ), our results showed that, when compared with the ND group, HFD intake modified the microbial diversity and, among the tested compounds, 6-gingerol changed the abundance of certain bacteria over two weeks, suggesting a disrupted gut environment.

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Gut Microbiota Restores Central Neuropeptide Deficits in Germ-Free Mice

“…Prior studies have also showed that specific gut microbiota modulation and presence of beneficial species into the gut, as measured through an increased Bacteroidetes/Firmicutes ratio, improves leptin response [ 42 ]. More specifically, species that are common in probiotics, such as Bifidobacterium and Lactobacillus, had beneficial effects on leptin sensitivity and were found to be decreased overall in leptin-deficient mice [ 43 , 44 ]. Therefore, it appears that the effects of gut microbiota on leptin responses is dependent on specific species as well as gut enterotypes, which can be altered by depletion or addition of probiotics.…”